Directed Evolution of Protein-Based Neurotransmitter Sensors for MRI [PDF]
, 2013 The production of contrast agents sensitive to neuronal signaling events is a rate-limiting step in the development of molecular-level functional magnetic resonance imaging (molecular fMRI) approaches for studying the brain.
A Jasanoff +11 more
core +1 more source
Modification of pancreatic lipase properties by directed molecular evolution [PDF]
Protein Engineering Design and Selection, 2010 Cystic fibrosis is associated with pancreatic insufficiency and acidic intraluminal conditions that limit the action of pancreatic enzyme replacement therapy, especially that of lipase. Directed evolution combined with rational design was used in the aim of improving the performances of the human pancreatic lipase at acidic pH.
Colin, D. +4 more
openaire +3 more sources
How special is the biochemical function of native proteins? [version 1; referees: 2 approved]
F1000Research, 2016 Native proteins perform an amazing variety of biochemical functions, including enzymatic catalysis, and can engage in protein-protein and protein-DNA interactions that are essential for life.
Jeffrey Skolnick, Mu Gao, Hongyi Zhou
doaj +1 more source
Protein engineering of Pseudomonas fluorescens peroxidase Dyp1B for oxidation of phenolic and polymeric lignin substrates [PDF]
, 2019 Directed evolution was applied to dye-decolourizing peroxidase Dyp1B from Pseudomonas fluorescens Pf-5, in order to enhance the activity for oxidation of phenolic and lignin substrates.
Ashley, Ben +6 more
core +2 more sources
Structure-Guided Directed Evolution of Highly Selective P450-Based Magnetic Resonance Imaging Sensors for Dopamine and Serotonin [PDF]
, 2012 New tools that allow dynamic visualization of molecular neural events are important for studying the basis of brain activity and disease. Sensors that permit ligand-sensitive magnetic resonance imaging (MRI) are useful reagents due to the noninvasive ...
Arnold, Frances H. +8 more
core +2 more sources
Evolution of a Landscape Phage Library in a Mouse Xenograft Model of Human Breast Cancer
Viruses, 2019 Peptide-displayed phage libraries are billion-clone collections of diverse chimeric bacteriophage particles, decorated by genetically fused peptides built from a random combination of natural amino acids.
James W. Gillespie +4 more
doaj +1 more source
CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors
Genome Biology, 2019 Increasing genetic diversity via directed evolution holds great promise to accelerate trait development and crop improvement. We developed a CRISPR/Cas-based directed evolution platform in plants to evolve the rice (Oryza sativa) SF3B1 spliceosomal ...
Haroon Butt +6 more
doaj +1 more source
Directed enzyme evolution: climbing fitness peaks one amino acid at a time [PDF]
, 2009 Directed evolution can generate a remarkable range of new enzyme properties. Alternate substrate specificities and reaction selectivities are readily accessible in enzymes from families that are naturally functionally diverse.
Arnold, Frances H., Tracewell, Cara A.
core +2 more sources
Exploiting Models of Molecular Evolution to Efficiently Direct Protein Engineering [PDF]
Journal of Molecular Evolution, 2010 Directed evolution and protein engineering approaches used to generate novel or enhanced biomolecular function often use the evolutionary sequence diversity of protein homologs to rationally guide library design. To fully capture this sequence diversity, however, libraries containing millions of variants are often necessary. Screening libraries of this
Megan F, Cole, Eric A, Gaucher
openaire +2 more sources
The molecular evolution of feathers with direct evidence from fossils [PDF]
Proceedings of the National Academy of Sciences, 2019 Significance During the dinosaur–bird transition, feathers of bird ancestors must have been molecularly modified to become biomechanically suitable for flight. We report molecular moieties in fossil feathers that shed light on that transition. Pennaceous feathers attached to the right forelimb of the Jurassic dinosaur
Yanhong Pan +15 more
openaire +2 more sources