Results 41 to 50 of about 3,475,040 (327)

LOVTRAP, An Optogenetic System for Photo-induced Protein Dissociation

Nature Methods, 2016
LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (Kd).

semanticscholar   +1 more source

Charge regulation: a generalized boundary condition?

, 2016
The three most commonly-used boundary conditions for charged colloidal systems are constant charge (insulator), constant potential (conducting electrode) and charge regulation (ionizable groups at the surface).
Andelman, David, Markovich, Tomer, Podgornik, Rudi   +2 more
core   +1 more source

Astrophysical fluid simulations of thermally ideal gases with non-constant adiabatic index: numerical implementation [PDF]

, 2015
An Equation of State (\textit{EoS}) closes the set of fluid equations. Although an ideal EoS with a constant \textit{adiabatic index} $\Gamma$ is the preferred choice due to its simplistic implementation, many astrophysical fluid simulations may benefit ...
Bodo, G., Massaglia, S., Mignone, A., Vaidya, B.   +3 more
core   +2 more sources

Generation of Red-Shifted Cameleons for Imaging Ca2+ Dynamics of the Endoplasmic Reticulum

Sensors, 2015
Cameleons are sophisticated genetically encoded fluorescent probes that allow quantifying cellular Ca2+ signals. The probes are based on Förster resonance energy transfer (FRET) between terminally located fluorescent proteins (FPs), which move together ...
Markus Waldeck-Weiermair, Helmut Bischof, Sandra Blass, Andras T. Deak, Christiane Klec, Thomas Graier, Clara Roller, Rene Rost, Emrah Eroglu, Benjamin Gottschalk, Nicole A. Hofmann, Wolfgang F. Graier, Roland Malli   +12 more
doaj   +1 more source

Force Modulating Dynamic Disorder: Physical Theory of Catch-slip bond Transitions in Receptor-Ligand Forced Dissociation Experiments

, 2006
Recently experiments showed that some adhesive receptor-ligand complexes increase their lifetimes when they are stretched by mechanical force, while the force increase beyond some thresholds their lifetimes decrease.
A. Messiah, E. B. Finger, Fei Liu, G. I. Bell, H. Risken, M. Dembo, Zhong-can Ou-Yang   +6 more
core   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

On the Electrochemical Detection of Alpha-Fetoprotein Using Aptamers: DNA Isothermal Amplification Strategies to Improve the Performance of Weak Aptamers

Biosensors, 2020
Affinity characterization is essential to develop reliable aptamers for tumor biomarker detection. For alpha-fetoprotein (AFP), a biomarker of hepatocellular carcinoma (HCC), two DNA aptamers were described with very different affinity.
Ramón Lorenzo-Gómez, Daniel González-Robles, Rebeca Miranda-Castro, Noemí de-los-Santos-Álvarez, María Jesús Lobo-Castañón   +4 more
doaj   +1 more source

Potentiometric investigation of acid-base equilibria of two new pyrimidine derivatives in various methanol-water media [PDF]

Journal of the Serbian Chemical Society, 2006
The acid-base properties of 1-amino-5-benzoyl-4-phenyl-1H-pyrimidine-2-one (L1) and 1-amino-5-benzoyl-4-phenyl-1H-pyrimidine-2-thione (L2) were investigated potentiometrically at an ionic strength of 0.10M(LiCl) in 19.8, 33.6 and 55.9 % (v/v) methanol ...
Kiliç Hasan, Birol Er
doaj   +1 more source

Stability of a Spherical Accretion Shock with Nuclear Dissociation

, 2009
We examine the stability of a standing shock wave within a spherical accretion flow onto a gravitating star, in the context of core-collapse supernova explosions.
Blondin, Blondin, Burrows, Burrows, Calder, Christopher Thompson, Davidson, Epstein, Fernández, Foglizzo, Foglizzo, Fryxell, Iwakami, Landau, Leveque, Marek, Ohnishi, Press, Rodrigo Fernández, Thompson, Yamasaki   +20 more
core   +1 more source

Structural insights into lacto‐N‐biose I recognition by a family 32 carbohydrate‐binding module from Bifidobacterium bifidum

FEBS Letters, EarlyView.
Bifidobacterium bifidum establishes symbiosis with infants by metabolizing lacto‐N‐biose I (LNB) from human milk oligosaccharides (HMOs). The extracellular multidomain enzyme LnbB drives this process, releasing LNB via its catalytic glycoside hydrolase family 20 (GH20) lacto‐N‐biosidase domain.
Xinzhe Zhang, Naoki Sunagawa, Toma Kashima, Kiyohiko Igarashi, Akimasa Miyanaga, Shinya Fushinobu   +5 more
wiley   +1 more source