Results 51 to 60 of about 1,952,840 (306)

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

open access: yesFEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav   +2 more
wiley   +1 more source

What and How the Accuracy of the Equilibrium Dissociation Constant of Affinity Complexes Depends on Fundamentally

open access: yes, 2023
 Figures in main text and supporting information (Origin and png files) for the manuscript of " What and How the Accuracy of the Equilibrium Dissociation Constant of Affinity Complexes Depends on Fundamentally"
TONG YE WANG (12959627)   +5 more
core   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

open access: yesFEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge   +6 more
wiley   +1 more source

The micro-dissociation rate constant of the LacI from the DNA.

open access: yes, 2016
The micro-dissociation rate constant of the LacI from the DNA.
Sen Hou (382797)   +8 more
core   +1 more source

L‐aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in Synechocystis sp. PCC6803

open access: yesFEBS Letters, EarlyView.
Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil   +3 more
wiley   +1 more source

İnvestigation of Changable of the Bicarbonate Dissociation Constant

open access: yesEurasian Journal of Medicine, 2019
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Yaşar Nuri Şahin   +2 more
doaj  

The Mutagenic Plasticity of the Cholera Toxin B-Subunit Surface Residues: Stability and Affinity

open access: yesToxins
Mastering selective molecule trafficking across human cell membranes poses a formidable challenge in healthcare biotechnology while offering the prospect of breakthroughs in drug delivery, gene therapy, and diagnostic imaging. The cholera toxin B-subunit
Cheuk W. Au   +5 more
doaj   +1 more source

How to measure and evaluate binding affinities

open access: yeseLife, 2020
Quantitative measurements of biomolecule associations are central to biological understanding and are needed to build and test predictive and mechanistic models.
Inga Jarmoskaite   +3 more
doaj   +1 more source

A modified RP-HPLC method for the determination of the pKa values of synthesized β-hydroxy-β-arylalkanoic acids [PDF]

open access: yesJournal of the Serbian Chemical Society, 2018
The pKa values of twelve β-hydroxy-β-arylalkanoic acids and ibuprofen were determined using a modified RP-HPLC method. The stationary phase was octadecyl modified (C-18) silica gel, and the mobile phases were mixtures of methanol and one of ten different
Savić Jelena S.   +4 more
doaj   +1 more source

Thermodynamics, stoichiometry, and dissociation constant of albumin with GML, lauric acid or glycerol.

open access: yes, 2016
Thermodynamics, stoichiometry, and dissociation constant of albumin with GML, lauric acid or glycerol.
Michael S. Zhang (3233793)   +1 more
core   +1 more source

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