How to measure and evaluate binding affinities
eLife, 2020 Quantitative measurements of biomolecule associations are central to biological understanding and are needed to build and test predictive and mechanistic models.
Inga Jarmoskaite +3 more
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The dimer-monomer equilibrium of SARS-CoV-2 main protease is affected by small molecule inhibitors
Scientific Reports, 2021 The maturation of coronavirus SARS-CoV-2, which is the etiological agent at the origin of the COVID-19 pandemic, requires a main protease Mpro to cleave the virus-encoded polyproteins. Despite a wealth of experimental information already available, there
Lucia Silvestrini +13 more
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Kinetic and Structural Analysis of the Mg2+ -binding Site of the Guanine Nucleotide-binding Protein p21 H-ras. [PDF]
, 1993 The coordination and binding of the Mg2+ ion in the nucleotide−binding site of p21 have been investigated using site−directed mutagenesis, kinetic methods, and phosphorous NMR.
Borasio, Gian Domenico +6 more
core
İnvestigation of Changable of the Bicarbonate Dissociation Constant
Eurasian Journal of Medicine, 2019 -
Yaşar Nuri Şahin +2 more
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The Mutagenic Plasticity of the Cholera Toxin B-Subunit Surface Residues: Stability and Affinity
ToxinsMastering selective molecule trafficking across human cell membranes poses a formidable challenge in healthcare biotechnology while offering the prospect of breakthroughs in drug delivery, gene therapy, and diagnostic imaging. The cholera toxin B-subunit
Cheuk W. Au +5 more
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A modified RP-HPLC method for the determination of the pKa values of synthesized β-hydroxy-β-arylalkanoic acids [PDF]
Journal of the Serbian Chemical Society, 2018 The pKa values of twelve β-hydroxy-β-arylalkanoic acids and ibuprofen were determined using a modified RP-HPLC method. The stationary phase was octadecyl modified (C-18) silica gel, and the mobile phases were mixtures of methanol and one of ten different
Savić Jelena S. +4 more
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, 2006 We calculate exactly both the microcanonical and canonical thermodynamic functions (TDFs) for a one-dimensional model system with piecewise constant Lennard-Jones type pair interactions.
A. Münster +13 more
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The influence of counter-ion adsorption on the ψ0/pH characteristics of insulator surfaces [PDF]
, 1983 The site-binding theory of Yates, Levine, and Healy is extended to include the possibility that counter-ion binding of anions and cations occurs at different distances from the insulator surface.
Bergveld, P. +2 more
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Interaction of GB1 with Metal Ions Studied by NMR Spectroscopy
Chinese Journal of Magnetic Resonance, 2018 B1 domain of staphylococcal protein G (GB1) is a widely used model protein for developing in vivo and in vitro protein structural determination methods based on paramagnetic nuclear magnetic resonance (NMR) such as pseudocontact chemical shift (PCS) and ...
CHENG Kai +3 more
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Perhitungan Energi Disosiasi Gugus Fungsi OH- dan PO4 3- Hidroksiapatit dengan Pemodelan Spektroskopi Inframerah Berbasis Particle Swarm Optimization (PSO) [PDF]
, 2013 ABSTRAK Hydroxyapatite Ca10(PO4)6(OH)2 is a calcium phosphate compound having biocompatibility properties. FTIR (Fourier Transform Infrared Spectroscopy) was used to determine the characteristics of the hydroxyapatite group.
Dahlan, Kiagus +2 more
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