Results 1 to 10 of about 31,432 (120)
Introduction of plasmid DNA into the trypanosomatid protozoan Crithidia fasciculata. [PDF]
Proceedings of the National Academy of Sciences, 1986 Crithidia fasciculata cells were treated with a plasmid (pDK96) containing pBR322 sequences, a Leishmania tarentolae maxicircle autonomously replicating sequence, and the bacterial gene for aminoglycoside 3' phosphotransferase I inserted between the yeast alcohol dehydrogenase 1 promotor and terminator sequences.
Larry Simpson, Dallas E. Hughes
openaire +3 more sources
Sequence specificity of DNA adenine methylase in the protozoan Tetrahymena thermophila [PDF]
Journal of Bacteriology, 1982 The sequence specificity of the Tetrahymena DNA-adenine methylase was determined by nearest-neighbor analyses of in vivo and in vitro methylated DNA. In vivo all four common bases were found to the 5' side of N6-methyladenine, but only thymidine was 3'. Homologous DNA already methylated in vivo and heterologous Micrococcus luteus DNA were methylated in
S Bromberg, K Pratt, S Hattman
openaire +3 more sources
Sensing DNA methylation in the protozoan parasite Entamoeba histolytica [PDF]
Molecular Microbiology, 2006 SummaryIn the protozoan parasite Entamoeba histolytica, 5‐methylcytosine (m5C) was found predominantly in repetitive elements. Its formation is catalysed by Ehmeth, a DNA methyltransferase that belongs to the Dnmt2 subfamily. Here we describe a 32 kDa nuclear protein that binds in vitro with higher affinity to the methylated form of a DNA encoding a ...
Ohad Fisher +5 more
openaire +3 more sources
Molecular Ecology, EarlyView., 2023 Abstract Malaria is a mosquito‐borne disease caused by protozoans of the genus Plasmodium that affects both humans and wildlife. The fitness consequences of infections by avian malaria are well known in birds, however, little information exists on its impact on mosquitoes.
Marta Garrigós +4 more
wiley +1 more source
An unusually compact ribosomal DNA repeat in the protozoanGiardia lamblia [PDF]
Nucleic Acids Research, 1987 The ribosomal RNA (rRNA) genes of the protozoan parasite Giardia lamblia have been analyzed with respect to size, composition and copy number. They are found to be remarkable in several respects. First, the rRNAs themselves are the smallest yet reported for any eukaryotic organism.
Ching C. Wang +3 more
openaire +3 more sources
DNA Vaccines against Protozoan Parasites: Advances and Challenges [PDF]
Journal of Biomedicine and Biotechnology, 2007 Over the past 15 years, DNA vaccines have gone from a scientific curiosity to one of the most dynamic research field and may offer new alternatives for the control of parasitic diseases such as leishmaniasis and Chagas disease. We review here some of the advances and challenges for the development of DNA vaccines against these diseases.
openaire +3 more sources
Telomeres of polytene chromosomes in a ciliated protozoan terminate in duplex DNA loops [PDF]
Proceedings of the National Academy of Sciences, 1999 The end of a telomeric DNA sequence isolated from a polytene chromosome of a hypotrichous ciliate folds back and hybridizes with downstream telomeric sequence to form a t loop that is stable in the absence of protein and DNA cross-linking. The single-stranded, telomeric DNA sequence at the end of a macronuclear molecule does not form a t loop but ...
D. M. Prescott, K.G. Murti
openaire +3 more sources
Development of a method to extract protozoan DNA from black soil
Parasite Epidemiology and Control, 2019 Microorganisms in environmental samples are identified by sequential screening, isolation, and culture steps, followed by the verification of physiological characteristics and morphological classification. Isolation and purification of Amoebae from soil samples is extremely complex, laborious, and time-consuming and require considerable expertise for ...
Kanako Yamanouchi +3 more
openaire +4 more sources
Albendazole induces oxidative stress and DNA damage in the parasitic protozoan Giardia duodenalis [PDF]
Frontiers in Microbiology, 2015 The control of Giardia duodenalis infections is carried out mainly by drugs, among these albendazole (ABZ) is commonly used. Although the cytotoxic effect of ABZ usually involves binding to β-tubulin, it has been suggested that oxidative stress may also play a role in its parasiticidal mechanism.
Rodrigo Martínez-Espinosa +3 more
openaire +4 more sources
Telomeric localization of the modified DNA base J in the genome of the protozoan parasite Leishmania [PDF]
Nucleic Acids Research, 2007 Base J or beta-d-glucosylhydroxymethyluracil is a DNA modification replacing a fraction of thymine in the nuclear DNA of kinetoplastid parasites and of Euglena. J is located in the telomeric sequences of Trypanosoma brucei and in other simple repeat DNA sequences.
Paul-André Genest +4 more
openaire +3 more sources