DNA Microarray Detection of 18 Important Human Blood Protozoan Species. [PDF]
Accurate detection of blood protozoa from clinical samples is important for diagnosis, treatment and control of related diseases. In this preliminary study, a novel DNA microarray system was assessed for the detection of Plasmodium, Leishmania ...
Mu-Xin Chen +9 more
doaj +5 more sources
Development of a method to extract protozoan DNA from black soil
Objectives: Microorganisms in environmental samples are identified by sequential screening, isolation, and culture steps, followed by the verification of physiological characteristics and morphological classification.
Kanako Yamanouchi +3 more
doaj +4 more sources
Protozoan ALKBH8 oxygenases display both DNA repair and tRNA modification activities.
The ALKBH family of Fe(II) and 2-oxoglutarate dependent oxygenases comprises enzymes that display sequence homology to AlkB from E. coli, a DNA repair enzyme that uses an oxidative mechanism to dealkylate methyl and etheno adducts on the nucleobases ...
Daria Zdżalik +10 more
doaj +7 more sources
Albendazole induces oxidative stress and DNA damage in the parasitic protozoan Giardia duodenalis [PDF]
The control of Giardia duodenalis infections is carried out mainly by drugs, among these albendazole (ABZ) is commonly used. Although the cytotoxic effect of ABZ usually involves binding to β-tubulin, it has been suggested that oxidative stress may also ...
Rodrigo eMartínez-Espinoza +3 more
doaj +4 more sources
DNA Vaccines against Protozoan Parasites: Advances and Challenges [PDF]
Over the past 15 years, DNA vaccines have gone from a scientific curiosity to one of the most dynamic research field and may offer new alternatives for the control of parasitic diseases such as leishmaniasis and Chagas disease. We review here some of the advances and challenges for the development of DNA vaccines against these diseases.
Eric Dumonteil
openaire +3 more sources
DNA Extraction from Protozoan Oocysts/Cysts in Feces for Diagnostic PCR
PCR detection of intestinal protozoa is often restrained by a poor DNA recovery or by inhibitors present in feces. The need for an extraction protocol that can overcome these obstacles is therefore clear. QIAamp® DNA Stool Mini Kit (Qiagen) was evaluated for its ability to recover DNA from oocysts/cysts directly from feces. Twenty-five Giardia-positive,
Yousry A. Hawash
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Sensing DNA methylation in the protozoan parasite Entamoeba histolytica [PDF]
Summary In the protozoan parasite Entamoeba histolytica , 5‐methylcytosine (m5C) was found predominantly in repetitive elements. Its formation is catalysed by Ehmeth, a DNA methyltransferase that belongs to the Dnmt2 subfamily.
Tal, Lavi +5 more
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Hsp90 and associates shaping parasite biology [PDF]
Hsp90 is considered to be the master regulator of chaperone activity within the cellular context. In addition to aiding client maturation and maintaining protein homeostasis, Hsp90 serves various non-canonical functions in model eukaryotes: ranging from ...
Abhilasha Gahlawat +2 more
doaj +2 more sources
Sequence specificity of DNA adenine methylase in the protozoan Tetrahymena thermophila [PDF]
The sequence specificity of the Tetrahymena DNA-adenine methylase was determined by nearest-neighbor analyses of in vivo and in vitro methylated DNA. In vivo all four common bases were found to the 5' side of N6-methyladenine, but only thymidine was 3'. Homologous DNA already methylated in vivo and heterologous Micrococcus luteus DNA were methylated in
S, Bromberg, K, Pratt, S, Hattman
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NMR structural analysis of DNA recognition by a novel Myb1 DNA-binding domain in the protozoan parasite Trichomonas vaginalis [PDF]
The transcription regulator, tvMyb1, is the first Myb family protein identified in Trichomonas vaginalis. Using an electrophoretic mobility shift assay, we defined the amino-acid sequence from Lys(35) to Ser(141) (tvMyb1(35-141)) as the minimal DNA-binding domain, encompassing two Myb-like DNA-binding motifs (designated as R2 and R3 motifs) and an ...
Lou, Yuan-Chao +6 more
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