Results 101 to 110 of about 31,432 (120)
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Dna-Protein Interactions at Telomeres in Ciliated Protozoans
Advances in Molecular and Cell Biology, 1988Publisher Summary This chapter discusses the DNA–protein complexes found at telomeres in the macronuclei of ciliated protozoans, with special attention given to studies in Oxytricha (a hypotrichous ciliate) and Tetrahymena (a holotrichous ciliate). The structure of telomeres has been largely elucidated by studies on macronuclear DNA from ciliated
Virginia A. Zakian +1 more
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Stable DNA transfection of the primitive protozoan pathogen Giardia lamblia
Molecular and Biochemical Parasitology, 1998We have developed a stable DNA transfection vector pRANneo for genetic manipulation of the primitive protozoan Giardia lamblia. pRANneo was constructed by replacing the protein coding region of a Giardia ran gene with a bacterial neomycin phosphotransferase gene (neo). This plasmid was electroporated into G. lamblia, and the transfectants were selected
Jung-Hsiang Tai +3 more
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DNA microarray-based detection and identification of waterborne protozoan pathogens
Journal of Microbiological Methods, 2010A DNA microarray with 21 oligonucleotide probes was developed to detect most of the common waterborne protozoan pathogens. The DNA microarray accurately identified 3 test protozoa strains based on the small subunit (SSU) rRNA gene sequence. The detection limit was approximately 1x10(3) target genes, or 50 Cryptosporidium parvum oocysts, per assay. As a
Peter Seto +2 more
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Stimulation of B lymphocytes, macrophages, and dendritic cells by protozoan DNA
Microbes and Infection, 2002This review summarizes recent work from our laboratory demonstrating the activation of B lymphocytes, macrophages and dendritic cells by DNA from three different protozoan parasites of cattle and humans that is qualitatively similar to the now well-described effects of CpG-containing bacterial DNA. This novel mechanism of protozoan parasite recognition
Ricardo S. Corral, Wendy C. Brown
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DNA methylation in vegetative and conjugating cells of a protozoan ciliate: Blepharisma japonicum [PDF]
Developmental Genetics, 1986 AbstractWe report here the presence of N6‐methyladenine (MeAde) in the macronuclear DNA (maDNA) of Blepharisma japonicum vegetative cells. We have further investigated the relationship between DNA methylation and cell union in cells activated for conjugation. Such activation was induced by treating cells of mating type I with complementary gamone 2. We
SALVINI, Mariangela +3 more
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Use of Hybridoma Antibodies and Recombinant DNA Technology in Protozoan Vaccine Development
Avian Diseases, 1986The use of hybridoma antibodies developed against the sporozoite stage of avian coccidia, coupled with genetic-engineering techniques, has made it possible to begin bird-immunization studies utilizing an Escherichia coli-elicited coccidial protein. The coccidia are currently controlled in the poultry industry by use of anticoccidial compounds, but it ...
P.C. Augustine, H.D. Danforth
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Fast and reliable extraction of protozoan parasite DNA from fecal specimens2
Molecular Diagnosis, 1999Polymerase chain reaction (PCR) detection of intestinal protozoa in fecal specimens is hampered by poor recovery of DNA and by the presence of PCR inhibitors. In this study we describe a novel method for DNA extraction from such specimens containing spores and oocysts of Enterocytozoon bieneusi and Cryptosporidium parvum, respectively.Extraction was ...
A Dasilva +6 more
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Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1988
1. A method for isolating DNA from Pneumocystis carinii is described. 2. The DNA content per nucleus is 0.22-0.34 pg. 3. This finding is consistent with other parasitic protozoa DNA content per nuclei.
M S Gradus, Michael S. Gilmore, M Lerner
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1. A method for isolating DNA from Pneumocystis carinii is described. 2. The DNA content per nucleus is 0.22-0.34 pg. 3. This finding is consistent with other parasitic protozoa DNA content per nuclei.
M S Gradus, Michael S. Gilmore, M Lerner
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DNA-dependent RNA Polymerase from a Protozoan, Tetrahymena pyriformis
The Journal of Biochemistry, 1975Three peaks of DNA-dependent RNA polymerase (EC 2.7.7.6) activity were resolved when the enzyme was prepared from the isolated macronuclei of Tetrahymena pyriformis GL(amicronucleate strain) and chromatographed on DEAE-Sephadex A25. They were eluted at around 0.05, 0.15, and 0.2 M of ammonium sulfate, and termed TIa, TIb, and TII, respectively.
Toru Higashinakagawa +2 more
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Talanta, 1997
An electrochemical hybridization biosensor was developed for the detection of short DNA fragments specific to the deadly waterborne pathogen Cryptosporidium. The sensor relies on the immobilization of a 38-mer oligonucleotide unique to the Cryptosporidium DNA onto the carbon-paste transducer, and employs a highly sensitive chronopotentiometric ...
Mark N. Flair +4 more
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An electrochemical hybridization biosensor was developed for the detection of short DNA fragments specific to the deadly waterborne pathogen Cryptosporidium. The sensor relies on the immobilization of a 38-mer oligonucleotide unique to the Cryptosporidium DNA onto the carbon-paste transducer, and employs a highly sensitive chronopotentiometric ...
Mark N. Flair +4 more
openaire +3 more sources