Results 271 to 280 of about 2,121,701 (315)

Cytoplasmic DNA-binding proteins

Experimental Cell Research, 1973
Abstract Cytoplasmic DNA-binding proteins were isolated from Chinese hamster liver, kidney and tissue culture cells by DNA-polyacrylamide chromatography. With homologous Chinese hamster DNA, and with calf thymus DNA, 1.4% of the proteins were bound to the column. With single-stranded DNA and with heterologous Micrococcus lysodeikticus DNA there was
S T, Vaughan, D E, Comings
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DNA-binding proteins of mammalian mitochondria

Mitochondrion, 2005
Acid-soluble proteins were isolated from liver and spleen mitochondria and their ability to form complexes with DNA was investigated. According to electrophoresis data, acid-soluble proteins include about 20 polypeptides ranging in the molecular mass from 10 to 120 kDa.
Michael P, Kutsyi, Natalia A, Gouliaeva, Elena A, Kuznetsova, Azhub I, Gaziev   +3 more
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DNA-Binding proteins in human serum

Biochemical and Biophysical Research Communications, 1975
Summary Human serum DNA-binding proteins were isolated by affinity chromatography on DNA-cellulose. These proteins represent a significant fraction (1.5%) of the serum proteins. The isolated proteins were quantitatively assayed by a nitrocellulose membrane filter technique using human DNA.
S P, Brehm, S O, Hoch, J A, Hoch
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DNA Binding by Proteins

Science, 1988
Study of proteins that recognize specific DNA sequences has yielded much information, but the field is still in its infancy. Already two major structural motifs have been discovered, the helix-turn-helix and zinc finger, and numerous examples of DNA-binding proteins containing either of them are known. The restriction enzyme Eco RI uses yet a different
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Affinity purification of DNA-binding proteins

Journal of Biochemical and Biophysical Methods, 2001
The focus of this review is on DNA affinity chromatography, which is the most powerful tool for purification of DNA binding proteins. The use of nonspecific-, sequence specific- and single stranded-DNA affinity columns in purification of various DNA binding proteins is discussed.
H, Gadgil, S A, Oak, H W, Jarrett
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Computational Design of DNA-Binding Proteins

2016
Predicting the outcome of engineered and naturally occurring sequence perturbations to protein-DNA interfaces requires accurate computational modeling technologies. It has been well established that computational design to accommodate small numbers of DNA target site substitutions is possible. This chapter details the basic method of design used in the
Summer, Thyme, Yifan, Song
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Ultrasensitive protein–DNA binding assays

Current Opinion in Biotechnology, 2003
Protein-DNA binding assays have been used in a variety of fields from fundamental studies regarding the binding process itself, to serving as probes for the detection, quantification and separation of target analytes. These assays have been used for the study of protein-DNA complex stoichiometry, the detection of DNA damage, and real-time separation of
Victor, Pavski, X Chris, Le
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DNA binding of a nonstructural reovirus protein

Canadian Journal of Biochemistry, 1981
The specific early inhibition of DNA synthesis in reovirus-infected cells suggests that the cell nucleus is a target for virus-induced damage. We have now examined the affinity of reovirus proteins for DNA, postulating that such affinity could provide a mechanism for the inhibition.
I H, Shelton, G J, Kasupski, C, Oblin, R, Hand   +3 more
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DNA Stability and DNA Binding Proteins

1996
Publisher Summary This chapter reviews the initial studies that focus on characterizing the factors that contribute to the stability of the genomes of hyperthermophiles, both extrinsic factors, such as the intracellular ionic environment and so-called histone like DNA binding proteins, and intrinsic factors, such as DNA base composition and topology.
R A, Grayling, K, Sandman, J N, Reeve
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Structure and function of DNA-binding proteins

Current Opinion in Genetics & Development, 1995
The past year has seen the determination of over three dozen new structures of DNA-binding proteins. Highlights of new structural motifs include the DNA-binding domain of p53 tumor suppressor and the amino-terminal fragment of Escherichia coli DNA topoisomerase I.
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