Results 21 to 30 of about 2,121,701 (315)
Biolayer interferometry for DNA-protein interactions.
PLoS ONE, 2022 Biolayer interferometry (BLI) is a widely utilized technique for determining macromolecular interaction dynamics in real time. Using changes in the interference pattern of white light reflected off a biosensor tip, BLI can determine binding parameters ...
John K Barrows, Michael W Van Dyke
doaj +1 more source
A linear model for transcription factor binding affinity prediction in protein binding microarrays. [PDF]
PLoS ONE, 2011 Protein binding microarrays (PBM) are a high throughput technology used to characterize protein-DNA binding. The arrays measure a protein's affinity toward thousands of double-stranded DNA sequences at once, producing a comprehensive binding specificity ...
Matti Annala +3 more
doaj +1 more source
A Protein-DNA Binding Site Prediction Method Based on Multi-View Feature Fusion of Adjacent Residue
IEEE Access, 2023 The interaction between proteins and DNA occurs widely during the replication and transcription of DNA and other life activities. Therefore, the identification of protein- and DNA-binding sites is important for the study of protein function and drug ...
Ji Yang, Shuning Zhang
doaj +1 more source
Rapid detection and purification of sequence specific DNA binding proteins using magnetic separation [PDF]
Journal of the Serbian Chemical Society, 2006 In this paper, a method for the rapid identification and purification of sequence specific DNA binding proteins based on magnetic separation is presented.
TIJANA SAVIC +6 more
doaj +3 more sources
Protein–DNA binding in high-resolution [PDF]
Critical Reviews in Biochemistry and Molecular Biology, 2015 Recent advances in experimental and computational methodologies are enabling ultra-high resolution genome-wide profiles of protein-DNA binding events. For example, the ChIP-exo protocol precisely characterizes protein-DNA cross-linking patterns by combining chromatin immunoprecipitation (ChIP) with 5' → 3' exonuclease digestion.
Shaun, Mahony, B Franklin, Pugh
openaire +2 more sources
MHJ_0461 is a multifunctional leucine aminopeptidase on the surface of Mycoplasma hyopneumoniae [PDF]
Open Biology, 2015 Aminopeptidases are part of the arsenal of virulence factors produced by bacterial pathogens that inactivate host immune peptides. Mycoplasma hyopneumoniae is a genome-reduced pathogen of swine that lacks the genetic repertoire to synthesize amino acids ...
Veronica M. Jarocki +7 more
doaj +1 more source
DNA-binding function of c-Myb R2R3 around thermal denaturation temperature
Biophysics and Physicobiology, 2021 The minimum DNA-binding domain of the transcriptional factor c-Myb R2R3 remarkably fluctuates in the solution. In the present study, we evaluated the protein fluctuation of R2R3 C130I mutant, R2R3*, on its DNA-binding and folding thermodynamics.
Maki Kawasaki, Masayuki Oda
doaj +1 more source
DNA-binding activity of papillomavirus proteins [PDF]
Journal of Virology, 1987 We demonstrate DNA binding by papillomavirus (PV) open reading frame (ORF) proteins that correspond to the early transforming and trans-activating (E6 and E2) and late structural regions (L2 and L1) from bovine PV type 1 and human PV types 6b and 16.
R G, Mallon +2 more
openaire +2 more sources
Calling cards for DNA-binding proteins [PDF]
Genome Research, 2007 Identifying genomic targets of transcription factors is fundamental for understanding transcriptional regulatory networks. Current technology enables identification of all targets of a single transcription factor, but there is no realistic way to achieve the converse: identification of all proteins that bind to a promoter of interest. We have developed
Wang, Haoyi +2 more
openaire +3 more sources
PLoS ONE, 2014 As more and more protein sequences are uncovered from increasingly inexpensive sequencing techniques, an urgent task is to find their functions. This work presents a highly reliable computational technique for predicting DNA-binding function at the level
Huiying Zhao +3 more
doaj +1 more source