Results 41 to 50 of about 2,121,701 (315)

Binding dynamics of a monomeric SSB protein to DNA : a single-molecule multi-process approach

, 2015
People Programme of the European Union’s Seventh Framework Programme [REA 334496 to B.E.B.]; Leonardo da Vinci European Union Programme (to M.F.G.); Wellcome Trust [099149/Z/12/Z, 091825/Z/10/Z]. Funding for open access charge: Wellcome Trust; University
J. Carlos Penedo, Maria Figueira-gonzalez, White, Malcolm F, Ackermann, Katrin, Katrin Ackermann, Penedo, Carlos, Peregrina, Jose Ramon, Bode, Bela Ernest, Michael J. Morten, Figueira-Gonzalez, Maria, Malcolm F. White, Bela E. Bode, Morten, Michael John, Jose R. Peregrina   +13 more
core   +1 more source

Characterization of the DNA binding activity of structural protein VP1 from chicken anaemia virus

BMC Veterinary Research, 2018
Background Chicken anaemia virus (CAV) is commonly found in poultry. VP1 is the sole structural protein of CAV, which is the major component responsible for capsid assembly. The CAV virion consists of the VP1 protein and a viral genome. However, there is
Guan-Hua Lai, Ming-Kuem Lin, Yi-Yang Lien, Jai-Hong Cheng, Fang-Chun Sun, Meng-Shiunn Lee, Hsi-Jien Chen, Meng-Shiou Lee   +7 more
doaj   +1 more source

A Protein in the Yeast Saccharomyces cerevisiae Presents DNA Binding Homology to the p53 Checkpoint Protein and Tumor Suppressor

Biomolecules, 2020
Saccharomyces cerevisiae does not contain a p53 homolog. Utilizing this yeast as an in vivo test tube model, our aim was to investigate if a yeast protein would show p53 DNA binding homology. Electrophoretic mobility shift analyses revealed the formation
Kanwal Farooqi, Marjan Ghazvini, Leah D. Pride, Louis Mazzella, David White, Ajay Pramanik, Jill Bargonetti, Carol Wood Moore   +7 more
doaj   +1 more source

Displacement of the canonical single-stranded DNA-binding protein in the Thermoproteales

, 2011
Single-stranded DNA binding proteins (SSBs) based on the OB-fold are considered ubiquitous in nature and play a central role in many DNA transactions including replication, recombination and repair.
Kroonin, Eugene V., Naismith, Jim, McMahon, Stephen, White, Malcolm F, Sonia Paytubi, Catherine H. Botting, Graham, Shirley, Botting, Catherine Helen, James H. Naismith, Malcolm F. White, Paytubi, Sonia, Shirley Graham, Liu, Huanting, Kira S. Makarova, Makarova, Kira S., Stephen A. McMahon, Huanting Liu, Eugene V. Koonin   +17 more
core   +1 more source

DNA-binding activity and subunit interaction of the mariner transposase [PDF]

, 2001
Mos1 is a member of the mariner/Tc1 family of transposable elements originally identified in Drosophila mauritiana. It has 28 bp terminal inverted repeats and like other elements of this type it transposes by a cut and paste mechanism, inserts at TA ...
Finnegan, D J, Dawson, A, Zhang, Lei
core   +1 more source

DNA Binding in High Salt: Analysing the Salt Dependence of Replication Protein A3 from the Halophile Haloferax volcanii [PDF]

Halophilic archaea maintain intracellular salt concentrations close to saturation to survive in high-salt environments and their cellular processes have adapted to function under these conditions.
Patoli, B, Bunting, KA, Winter, JA
core   +1 more source

Characterisation of Bombyx mori odorant-binding proteins reveals that a general odorant-binding protein discriminates between sex pheromone components [PDF]

, 2009
In many insect species, odorant-binding proteins (OBPs) are thought to be responsible for the transport of pheromones and other semiochemicals across the sensillum lymph to the olfactory receptors (ORs) within the antennal sensilla.
Field, L.M., Pickett, J. A., Dufour, S. G. L., Zhou, J.J., Keep, N. H., Dufour, Samuel, Field, Linda M., He, X., Hooper, A.M., Hooper, A. M., Pickett, John A., Pickett, J.A., He, Xiaoli, Dufour, S., Field, L. M., Keep, Nicholas H., Robertson, G., Robertson, Giles, Zhou, J-J., Hooper, Antony M., Zhou, Jing-Jiang   +20 more
core   +1 more source

DNA-binding residues and binding mode prediction with binding-mechanism concerned models

BMC Genomics, 2009
Background Protein-DNA interactions are essential for fundamental biological activities including DNA transcription, replication, packaging, repair and rearrangement.
Oyang Yen-Jen, Liu Yu-Cheng, Huang Chun-Chin, Huang Yu-Feng, Huang Chien-Kang   +4 more
doaj   +1 more source

Single-molecule dynamics and genome-wide transcriptomics reveal that NF-kB (p65)-DNA binding times can be decoupled from transcriptional activation.

PLoS Genetics, 2019
Transcription factors (TFs) regulate gene expression in both prokaryotes and eukaryotes by recognizing and binding to specific DNA promoter sequences. In higher eukaryotes, it remains unclear how the duration of TF binding to DNA relates to downstream ...
Andrea Callegari, Christian Sieben, Alexander Benke, David M Suter, Beat Fierz, Davide Mazza, Suliana Manley   +6 more
doaj   +1 more source

Liquid chemiluminescent DNA pull-down assay to measure nuclear receptor-DNA binding in solution

BioTechniques, 2008
Electrophoretic mobility shift assays (EMSAs) are commonly used to investigate protein-DNA binding in vitro. However, EMSA can generate considerable amounts of undesirable waste, particularly when toxic compounds are examined.
Toshiharu Iwasaki, Wataru Miyazaki, Nana Rokutanda, Noriyuki Koibuchi   +3 more
doaj   +1 more source