Results 41 to 50 of about 2,258,474 (352)

Liquid chemiluminescent DNA pull-down assay to measure nuclear receptor-DNA binding in solution

BioTechniques, 2008
Electrophoretic mobility shift assays (EMSAs) are commonly used to investigate protein-DNA binding in vitro. However, EMSA can generate considerable amounts of undesirable waste, particularly when toxic compounds are examined.
Toshiharu Iwasaki, Wataru Miyazaki, Nana Rokutanda, Noriyuki Koibuchi   +3 more
doaj   +1 more source

Survey of variation in human transcription factors reveals prevalent DNA binding changes [PDF]

, 2016
Published in final edited form as: Science. 2016 Mar 25; 351(6280): 1450–1454. Published online 2016 Mar 24. doi: 10.1126/science.aad2257Sequencing of exomes and genomes has revealed abundant genetic variation affecting the coding sequences of human ...
Barrera, Luis A., Bulyk, Martha L., Cotsapas, Chris, Daly, Mark J., Gisselbrecht, Stephen S., Gordan, Raluxa, Hao, Tong, Hill, David E., Inukai, Sachi, Kellis, Manolis, Kock, Kian Hong, Kurland, Jesse V., Mariani, Luca, Rogers, Julia M., Rossin, Elizabeth J., Sahni, Nidhi, Shokri, Leila, Siggers, Trevor, Vedenko, Anastasia, Vidal, Marc, Woodard, Jaie, Yi, Song   +21 more
core   +1 more source

Dynamics of \u3cem\u3eE. Coli\u3c/em\u3e Single Stranded DNA Binding (SSB) Protein-DNA Complexes [PDF]

, 2018
Single stranded DNA binding proteins (SSB) are essential to the cell as they stabilize transiently open single stranded DNA (ssDNA) intermediates, recruit appropriate DNA metabolism proteins, and coordinate fundamental processes such as replication ...
Antony, Edwin, Lohman, Timothy M.
core   +1 more source

Two time constants for the binding of proteins to DNA from micromechanical data [PDF]

, 2000
Recent experimental advances allow the direct measurement of the force/extension behavior for DNA in the presence of strongly binding proteins. Such experiments reveal information about the cooperative mechanism of protein binding.
Turner, Matthew S.
core   +2 more sources

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

DeepMC-iNABP: Deep learning for multiclass identification and classification of nucleic acid-binding proteins

Computational and Structural Biotechnology Journal, 2022
Nucleic acid-binding proteins (NABPs), including DNA-binding proteins (DBPs) and RNA-binding proteins (RBPs), play vital roles in gene expression. Accurate identification of these proteins is crucial.
Feifei Cui, Shuang Li, Zilong Zhang, Miaomiao Sui, Chen Cao, Abd El-Latif Hesham, Quan Zou   +6 more
doaj   +1 more source

Reciprocal control of viral infection and phosphoinositide dynamics

FEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

Computational study of associations between histone modification and protein-DNA binding in yeast genome by integrating diverse information

BMC Genomics, 2011
Background In parallel with the quick development of high-throughput technologies, in vivo (vitro) experiments for genome-wide identification of protein-DNA interactions have been developed.
Wang Junbai
doaj   +1 more source

Knockdown of Ubiquitin-Specific Protease 53 Enhances the Radiosensitivity of Human Cervical Squamous Cell Carcinoma by Regulating DNA Damage-Binding Protein 2

Technology in Cancer Research & Treatment, 2020
Background: Cervical cancer ranks fourth in incidence and mortality among women. Ubiquitin-specific protein 53 binds to damage-specific DNA binding protein 2 and affects the biological properties of colon cancer.
Qifen Zhou MM, Xiongbo Yao MM, Chunlin Wu AP, Shaohua Chen MM, Dage Fan MM   +4 more
doaj   +1 more source

Non-additivity in protein-DNA binding [PDF]

Bioinformatics, 2005
Localizing protein binding sites within genomic DNA is of considerable importance, but remains difficult for protein families, such as transcription factors, which have loosely defined target sequences. It is generally assumed that protein affinity for DNA involves additive contributions from successive nucleotide pairs within the target sequence. This
O'Flanagan, Ra, Paillard, G., Lavery, R., Sengupta, Am   +3 more
openaire   +3 more sources