Results 31 to 40 of about 2,121,701 (315)

Liquid Luminescent DNA-precipitation Assay

open access: yesBio-Protocol, 2016
Working on transcription factors requires studying interactions between protein and DNA. After identification of putative binding-sequences and motifs, Electrophoretic Mobility Shift Assay (EMSA) experiment is classically used to determine specific ...
Frédérique Végran   +3 more
doaj   +1 more source

Sequence specificity of single-stranded DNA-binding proteins:a novel DNA microarray approach [PDF]

open access: yes, 2007
We have developed a novel DNA microarray-based approach for identification of the sequence-specificity of single-stranded nucleic-acid-binding proteins (SNABPs).
Estibeiro, Peter   +17 more
core   +1 more source

Non-additivity in protein-DNA binding [PDF]

open access: yesBioinformatics, 2005
Localizing protein binding sites within genomic DNA is of considerable importance, but remains difficult for protein families, such as transcription factors, which have loosely defined target sequences. It is generally assumed that protein affinity for DNA involves additive contributions from successive nucleotide pairs within the target sequence. This
Ruadhan A. O'Flanagan   +3 more
openaire   +3 more sources

Two time constants for the binding of proteins to DNA from micromechanical data [PDF]

open access: yes, 2000
Recent experimental advances allow the direct measurement of the force/extension behavior for DNA in the presence of strongly binding proteins. Such experiments reveal information about the cooperative mechanism of protein binding.
Turner, Matthew S., Matthew S. Turner
core   +1 more source

Comparative footprinting of DNA-binding proteins [PDF]

open access: yesBioinformatics, 2006
Abstract Motivation: Comparative modelling is a computational method used to tackle a variety of problems in molecular biology and biotechnology. Traditionally it has been applied to model the structure of proteins on their own or bound to small ligands, although more recently it has also been used to model protein-protein interfaces ...
Bruno Contreras-Moreira   +1 more
openaire   +3 more sources

Helicase binding to DnaI exposes a cryptic DNA-binding site during helicase loading in Bacillus subtilis [PDF]

open access: yes, 1981
The Bacillus subtilis DnaI, DnaB and DnaD proteins load the replicative ring helicase DnaC onto DNA during priming of DNA replication. Here we show that DnaI consists of a C-terminal domain (Cd) with ATPase and DNA-binding activities and an N-terminal ...
Shi, Wei   +999 more
core   +1 more source

Luminescent detection of DNA-binding proteins [PDF]

open access: yesNucleic Acids Research, 2011
Transcription factors play a central role in cell development, differentiation and growth in biological systems due to their ability to regulate gene expression by binding to specific DNA sequences within the nucleus. The dysregulation of transcription factor signaling has been implicated in the pathogenesis of a number of cancers, developmental ...
Chan, DSH   +5 more
openaire   +4 more sources

ProDFace: A web-tool for the dissection of protein-DNA interfaces

open access: yesFrontiers in Molecular Biosciences, 2022
Protein-DNA interactions play a crucial role in gene expression and regulation. Identifying the DNA binding surface of proteins has long been a challenge–in comparison to protein-protein interactions, limited progress has been made in the development of ...
Arumay Pal   +2 more
doaj   +1 more source

An immunochemistry-based screen for chemical inhibitors of DNA-protein interactions and its application to human CGGBP1

open access: yesBMC Cancer, 2020
Background Inhibition of DNA-binding of proteins by small-molecule chemicals holds immense potential in manipulating the activities of DNA-binding proteins.
Manthan Patel   +3 more
doaj   +1 more source

Predicting target DNA sequences of DNA-binding proteins based on unbound structures. [PDF]

open access: yesPLoS ONE, 2012
DNA-binding proteins such as transcription factors use DNA-binding domains (DBDs) to bind to specific sequences in the genome to initiate many important biological functions.
Chien-Yu Chen   +5 more
doaj   +1 more source

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