Results 81 to 90 of about 2,258,474 (352)

Interaction of the 89K murine cytomegalovirus immediate-early protein with core histones [PDF]

, 1988
The conditions that permit the interaction of immediate-early proteins of murine cytornegalovirus (MCMV) with DNA were studied. Chromatography of extracts from infected cells on MCMV DNA cellulose and calf thymus DNA cellulose showed that pp89, the ...
Alberts, Bookout, Ebeling, Einck, Everett, Faber, Freeman, Green, Günther M. Keil, Hay, Hermiston, Isenberg, Kedes, Keil, Keil, Keil, Keil, Konrad Munch, Koszinowski, Koszinowski, Koszinowski, Kristie, Kristie, Martin Messerle, O'Hare, Ohlenbusch, Purifoy, Reddehase, Rigby, Stein, Tevethia, Ulrich H. Koszinowski, Weissbach   +32 more
core   +1 more source

Methylated DNA-Binding Proteins from Arabidopsis [PDF]

Plant Physiology, 2003
Abstract The 5-methylcytosines (m5C) play a critical role in epigenetic control, often being recognized by proteins containing a methyl-CpG-binding domain (MBD). Database screening has identified at least 12 putative methyl-CpG-binding proteins from Arabidopsis; we have isolated corresponding cDNAs for seven of them. Despite variation in
Mikako, Ito, Akiko, Koike, Nozomu, Koizumi, Hiroshi, Sano   +3 more
openaire   +2 more sources

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Interpretable protein-DNA interactions captured by structure-sequence optimization

eLife
Sequence-specific DNA recognition underlies essential processes in gene regulation, yet methods for simultaneous predictions of genomic DNA recognition sites and their binding affinity remain lacking. Here, we present the Interpretable protein-DNA Energy
Yafan Zhang, Irene Silvernail, Zhuyang Lin, Xingcheng Lin   +3 more
doaj   +1 more source

Targeting the OB-Folds of Replication Protein A with Small Molecules

Journal of Nucleic Acids, 2010
Replication protein A (RPA) is the main eukaryotic single-strand (ss) DNA-binding protein involved in DNA replication and repair. We have identified and developed two classes of small molecule inhibitors (SMIs) that show in vitro inhibition of the RPA ...
Victor J. Anciano Granadillo, Jennifer N. Earley, Sarah C. Shuck, Millie M. Georgiadis, Richard W. Fitch, John J. Turchi   +5 more
doaj   +1 more source

Determination of Adeno-associated Virus Rep DNA Binding Using Fluorescence Anisotropy

Bio-Protocol, 2017
Quantitative measurement of proteins binding to DNA is a requisite to fully characterize the structural determinants of complex formation necessary to understand the DNA transactions that regulate cellular processes.
Francisco Zarate-Perez, Vishaka Santosh, Martino Bardelli, Leticia Agundez, R. Michael Linden, Els Henckaerts, Carlos Escalante   +6 more
doaj   +1 more source

Prediction of mammalian tissue-specific CLOCK–BMAL1 binding to E-box DNA motifs

Scientific Reports, 2023
The Brain and Muscle ARNTL-Like 1 protein (BMAL1) forms a heterodimer with either Circadian Locomotor Output Cycles Kaput (CLOCK) or Neuronal PAS domain protein 2 (NPAS2) to act as a master regulator of the mammalian circadian clock gene network.
Daniel Marri, David Filipovic, Omar Kana, Shelley Tischkau, Sudin Bhattacharya   +4 more
doaj   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Using electrostatic potentials to predict DNA-binding sites on DNA-binding proteins [PDF]

Nucleic Acids Research, 2003
A method to detect DNA-binding sites on the surface of a protein structure is important for functional annotation. This work describes the analysis of residue patches on the surface of DNA-binding proteins and the development of a method of predicting DNA-binding sites using a single feature of these surface patches. Surface patches and the DNA-binding
Jones, S, Shanahan, H P, Berman, H M, Thornton, J M   +3 more
openaire   +8 more sources

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source