Results 71 to 80 of about 2,121,701 (315)

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Identification of essential and non-essential single-stranded DNA-binding proteins in a model archaeal organism

, 2011
This work is supported by the The Scottish Universities Life Sciences Alliance (SULSA)Single-stranded DNA-binding proteins (SSBs) play vital roles in all aspects of DNA metabolism in all three domains of life and are characterized by the presence of one ...
Skowyra, Agnieszka, Stuart A. MacNeill, Agnieszka Skowyra, MacNeill, Stuart   +3 more
core   +1 more source

Dynamic distribution of SeqA protein across the chromosome of Escherichia coli K-12 [PDF]

, 2010
The bacterial SeqA protein binds to hemi-methylated GATC sequences that arise in newly synthesized DNA upon passage of the replication machinery. In Escherichia coli K-12, the single replication origin oriC is a well-characterized target for SeqA, which ...
Stephen J. W. Busby, Nigel P. Dyer, Sascha Ott, Sánchez-Romero, María Antonia, Dyer, NP, Millard, AD, Sanchez-Romero, MA, María Antonia Sánchez-Romero, Busby, Stephen; id_orcid, Ott, S, Grainger, David, Dyer, Nigel, Millard, Andrew D., David C. Grainger, Ott, Sascha, Andrew D. Millard, Busby, S. (Stephen), Grainger, David C.   +17 more
core   +1 more source

The Characteristics of New SSB Proteins from Metagenomic Libraries and Their Use in Biotech Applications

Proceedings, 2020
Single-stranded DNA binding proteins (SSBs) bind to single-stranded DNA in a sequence-independent manner to prevent formation of secondary structures and protect DNA from nuclease degradation.
Olesia Werbowy, Aleksandra Stefańska-Kaźmierczak, Agata Jurczak-Kurek, Karolina Kwiatkowska-Semrau, Anna-Karina Kaczorowska, Magdalena Płotka, Olafur H. Fridjonsson, Gudmundur O. Hreggvidsson, Slawomir Dąbrowski, Tadeusz Kaczorowski   +9 more
doaj   +1 more source

Electrical Probes of DNA-Binding Proteins [PDF]

, 2017
A DNA electrochemistry platform has been developed to probe proteins bound to DNA electrically. Here gold electrodes are modified with thiol-modified DNA, and DNA charge transport chemistry is used to probe DNA binding and enzymatic reaction both with redox-silent and redox-active proteins.
Barton, Jacqueline K., Bartels, Phillip L., Deng, Yingxin, O'Brien, Elizabeth   +3 more
openaire   +3 more sources

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Phosphorylation of Adenovirus DNA-binding Protein

Journal of General Virology, 1989
Evidence is presented here which indicates that the adenovirus DNA-binding protein (DBP) is phosphorylated at a tyrosine residue early in infection. This was suggested by the discovery that a proportion of the label in 32P-labelled DBP was resistant to alkali, and was substantiated by acid hydrolysis of DBP immunoprecipitates and by immunoblotting with
W C, Russell, A, Webster, I R, Leith, G D, Kemp   +3 more
openaire   +2 more sources

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Single molecule force spectroscopy on ligand-DNA complexes: from molecular binding mechanisms to biosensor applications

, 2004
Ros R, Eckel R, Bartels FW, et al. Single molecule force spectroscopy on ligand-DNA complexes: from molecular binding mechanisms to biosensor applications. Journal of Biotechnology.
Baumgarth, Birgit, Wilking, Sven David, Ros, Robert, Bartels, Frank Wilco, Sischka, Andy, Eckel, Rainer, Pühler, Alfred ; https://orcid.org/, Sewald, Norbert ; https://orcid.org/, Becker, Anke, Anselmetti, Dario ; https://orcid.org/   +9 more
core   +1 more source

Grafting Miniature DNA Binding Proteins [PDF]

Chemistry & Biology, 2004
Miniature proteins serve as leads for biological and medicinal applications by positioning all amino acids necessary for biomolecular recognition on a compact protein structure. Protein grafting was recently used to create miniature helical proteins with high DNA binding affinity and specificity.
openaire   +2 more sources