Results 21 to 30 of about 219,109 (274)

First-passage problems in DNA replication: effects of template tension on stepping and exonuclease activities of a DNA polymerase motor [PDF]

Journal of Physics: Condensed Matter, vol.25, 374105 (2013), 2013
A DNA polymerase (DNAP) replicates a template DNA strand. It also exploits the template as the track for its own motor-like mechanical movement. In the polymerase mode it elongates the nascent DNA by one nucleotide in each step. But, whenever it commits an error by misincorporating an incorrect nucleotide, it can switch to an exonuclease mode.
arxiv   +1 more source

Stochastic resetting in backtrack recovery by RNA polymerases [PDF]

Phys. Rev. E 93, 062411 (2016), 2016
Transcription is a key process in gene expression, in which RNA polymerases produce a complementary RNA copy from a DNA template. RNA polymerization is frequently interrupted by backtracking, a process in which polymerases perform a random walk along the DNA template.
arxiv   +1 more source

T4 DNA polymerase improves the efficiency of multiple site-directed mutagenesis

Biotechnology & Biotechnological Equipment, 2016
Site-directed mutagenesis (SDM) is a useful tool to study the functions of regulatory sequences of DNA and RNA, and the structures and functions of proteins. Numerous methods have been developed for either single or multiple SDM (MSDM).
Wei Guo, Shuhui Sun, Binghua Xie, Xiao-Jing Zhu, Zhong-Min Dai   +4 more
doaj   +1 more source

The Pseudorabies Virus DNA Polymerase Accessory Subunit UL42 Directs Nuclear Transport of the Holoenzyme

Frontiers in Microbiology, 2016
Pseudorabies virus (PRV) DNA replication occurs in the nuclei of infected cells and requires the viral DNA polymerase. The PRV DNA polymerase comprises a catalytic subunit, UL30, and an accessory subunit, UL42, that confers processivity to the enzyme ...
Yi-Ping eWang, Wen-Juan eDu, Li-Ping eHuang, Yan-Wu eWei, Hong-Li eWu, Li eFeng, Chang-Ming eLiu   +6 more
doaj   +1 more source

A DCL3 dicing code within Pol IV-RDR2 transcripts diversifies the siRNA pool guiding RNA-directed DNA methylation

eLife, 2022
In plants, selfish genetic elements, including retrotransposons and DNA viruses, are transcriptionally silenced by RNA-directed DNA methylation. Guiding the process are short interfering RNAs (siRNAs) cut by DICER-LIKE 3 (DCL3) from double-stranded ...
Andrew Loffer, Jasleen Singh, Akihito Fukudome, Vibhor Mishra, Feng Wang, Craig S Pikaard   +5 more
doaj   +1 more source

Inhibitors of HIV-1 Reverse Transcriptase—Associated Ribonuclease H Activity

Biology, 2012
HIV-1 enzyme reverse transcriptase (RT) is a major target for antiviral drug development, with over half of current FDA-approved therapeutics against HIV infection targeting the DNA polymerase activity of this enzyme. HIV-1 RT is a multifunctional enzyme
Michael A. Parniak, Stefan G. Sarafianos, Rieko Ishima, Krystal LaBarge, Tatiana Ilina   +4 more
doaj   +1 more source

The Inhibitory Action of Kohamaic Acid A Derivatives on Mammalian DNA Polymerase β

Molecules, 2008
We previously isolated a novel natural product, designated kohamaic acid A (KA-A, compound 1), as an inhibitor of the first cleavage of fertilized sea urchin eggs, and found that this compound could selectively inhibit the activities of mammalian DNA ...
Yoshiyuki Mizushina, Daisuke Manita, Toshifumi Takeuchi, Yuko Kumamoto-Yonezawa, Yuki Matsui, Fumio Sugawara, Masaharu Takemura, Hirosato Takikawa, Hiromi Yoshida, Mitsuru Sasaki   +9 more
doaj   +1 more source

Engagement of Components of DNA-Break Repair Complex and NFκB in Hsp70A1A Transcription Upregulation by Heat Shock. [PDF]

PLoS ONE, 2017
An involvement of components of DNA-break repair (DBR) complex including DNA-dependent protein kinase (DNA-PK) and poly-ADP-ribose polymerase 1 (PARP-1) in transcription regulation in response to distinct cellular signalling has been revealed by ...
Joyita Hazra, Pooja Mukherjee, Asif Ali, Soumita Poddar, Mahadeb Pal   +4 more
doaj   +1 more source

Error correction during DNA replication [PDF]

Physical Review E, VOL.86, 011913 (2012), 2011
DNA polymerase (DNAP) is a dual-purpose enzyme that plays two opposite roles in two different situations during DNA replication. It plays its normal role as a {\it polymerase} catalyzing the elongation of a new DNA molecule by adding a monomer. However, it can switch to the role of an {\it exonuclease} and shorten the same DNA by cleavage of the last ...
arxiv   +1 more source

What influences DNA replication rate in budding yeast? [PDF]

PLoS ONE, 2010
BACKGROUND: DNA replication begins at specific locations called replication origins, where helicase and polymerase act in concert to unwind and process the single DNA filaments. The sites of active DNA synthesis are called replication forks.
Thomas W Spiesser, Christian Diener, Matteo Barberis, Edda Klipp   +3 more
doaj   +1 more source