Results 171 to 180 of about 54,943 (215)
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Mechanisms of insertions at a DNA double-strand break
Molecular Cell, 2023Insertions and deletions (indels) are common sources of structural variation, and insertions originating from spontaneous DNA lesions are frequent in cancer. We developed a highly sensitive assay called insertion and deletion sequencing (Indel-seq) to monitor rearrangements in human cells at the TRIM37 acceptor locus that reports indels stemming from ...
Jaewon, Min +10 more
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DNA double‐strand breaks in mutagenesis
Environmental and Molecular Mutagenesis, 1993The DNA-double-strand break (dsb) appears to be the major lesion involved in chromosomal aberration formation. It is also very likely that the DNA dsb is the dominant lesion involved in radiation-induced mutagenesis. The dsb has been implicated as the primary lesion responsible for the induction of large deletions and, to a lesser extent, large ...
J W, Phillips, W F, Morgan
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DNA Double Strand Breaks and Chromosomal Aberrations
Cytogenetic and Genome Research, 2010DNA double strand breaks (DSBs) are ultimate lesions for the formation of chromosomal aberrations (CAs). The formation of CAs is dependent on many factors; some of these are discussed in this review. FISH methodologies have uncovered CA types which cannot be seen with the classical staining methods, and thereby widened our understanding of the origin ...
G. Obe, DURANTE, MARCO
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Ubiquitylation in DNA double-strand break repair
DNA Repair, 2021Genome integrity is constantly challenged by various DNA lesions with DNA double-strand breaks (DSBs) as the most cytotoxic lesions. In order to faithfully repair DSBs, DNA damage response (DDR) signaling networks have evolved, which organize many multi-protein complexes to deal with the encountered DNA damage.
Mengfan, Tang, Siting, Li, Junjie, Chen
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DNA Double-Strand Break Repair Assay
Cold Spring Harbor Protocols, 2017DNA double-strand breaks (DSBs), arising during normal DNA metabolism or following exposure to mutagenic agents such as ionizing radiation can lead to chromosomal rearrangements and genome instability, and are potentially lethal if unrepaired. Therefore, understanding the mechanisms of DSB repair and misrepair, and identifying the factors involved in ...
Chen-Chun, Pai +2 more
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DNA Double Strand Break Induction in Yeast
Radiation Protection Dosimetry, 2002The induction of DNA double strand breaks (DSBs) by accelerated heavy ions was systematically measured in diploid yeast cells. Particles were provided by the accelerators at GSI, Darmstadt, and HMI, Berlin. DNA was separated using pulsed field gel electrophoresis and the intensity of the largest bands used to determine the loss of molecular weight ...
J, Kiefer, R, Egenolf, S E, Ikpeme
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Coping with DNA double strand breaks
DNA Repair, 2010The repair of DNA double strand breaks (dsb) is important for maintaining the physical and genetic integrity of the genome. Moreover, in humans it is associated with the prevention of diseases such as immune deficiencies and cancer. This review briefly explores the fundamental strategies for repairing dsb, examines how cells maximize the fidelity of ...
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Overview of DNA damage and double-strand breaks
2022DNA is under a variety of assaults. As a result, different damages accumulate on DNA. These include base changes, single-strand breaks and double-strand breaks. In this volume and also briefly in the following volume, we discuss DNA damage and double-strand breaks. In particular, we focus on double-strand breaks.
Fuyuhiko, Tamanoi, Kenichi, Yoshikawa
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Processing of DNA Double-Strand Breaks in Yeast
2018Single-stranded DNA (ssDNA) intermediates are essential for homology-dependent repair of DNA double-strand breaks (DSBs) and for the DNA damage response. Here we describe methods routinely used to identify ssDNA intermediates formed by end processing of site-specific DSBs in Saccharomyces cerevisiae.
Robert, Gnügge +2 more
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Induction and Repair of DNA Double-Strand Breaks
Radiation Research, 1993Induction and repair of DNA double-strand breaks (DSBs) was measured using a pulsed-field gel electrophoresis system. A cell line of methotrexate-resistant EMT-6 cells that contain numerous double-minutes (DMs) 3 million base pairs in size was employed.
B, Nevaldine +5 more
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