Results 131 to 140 of about 108,714 (328)

Irradiation and Alterations in Hippocampal DNA Methylation

Epigenomes
The response of the brain to radiation is important for cancer patients receiving whole or partial brain irradiation or total body irradiation, those exposed to irradiation as part of a nuclear accident or a nuclear war or terrorism event, and for ...
Soren Impey, Jacob Raber
doaj   +1 more source

Effect of Sequence on the Conformation of DNA Holliday Junctions

, 2003
Structures of the DNA sequences d(CCGGCGCCGG) and d(CCAGTACbr5UGG) are presented here as four-way Holliday junctions in their compact stacked-X forms, with antiparallel alignment of the DNA strands.
Hays, Franklin A., Ho, P. Shing, Vargason, Jeff   +2 more
core   +1 more source

Phase Separation of Nucleic Acids: Mechanisms, Properties, and Applications

Angewandte Chemie International Edition, EarlyView.
Recent discoveries have shown that single‐stranded long‐chain nucleic acids can undergo temperature‐induced phase separation, enabling formation of micrometer‐sized condensates. This Minireview discusses the current mechanistic understanding of this phenomenon, highlights strategies for controlling the physical and chemical properties of these ...
Weixiang Chen, Johann Fritzen, Andreas Walther   +2 more
wiley   +1 more source

Enhanced C‐To‐T and A‐To‐G Base Editing in Mitochondrial DNA with Engineered DdCBE and TALED

Advanced Science
Mitochondrial base editing with DddA‐derived cytosine base editor (DdCBE) is limited in the accessible target sequences and modest activity. Here, the optimized DdCBE tools is presented with improved editing activity and expanded C‐to‐T targeting scope ...
Yinghui Wei, Ming Jin, Shuhong Huang, Fangyao Yao, Ningxin Ren, Kun Xu, Shangpu Li, Pengfei Gao, Yingsi Zhou, Yulin Chen, Hui Yang, Wen Li, Chunlong Xu, Meiling Zhang, Xiaolong Wang   +14 more
doaj   +1 more source

The Role of miRNAs in Chicken Immune Regulation and Prospects for Disease‐Resistant Breeding

Animal Research and One Health, EarlyView.
A schematic workflow illustrating the screening of disease‐resistant miRNAs and the generation of miRNA‐based disease‐resistant chickens via PGC‐mediated germline genome editing. ABSTRACT MicroRNAs (miRNAs) are emerging as pivotal regulators of the immune system, playing a decisive role in shaping disease resistance in chicken.
Qiangzhou Wang, Weiran Wu, Qingqing Cai, Jiaxing Wang, Qi Gao, Muhammad Iftikhar, Yulin Bi, Hao Bai, Lihong Gu, Guobin Chang, Shihao Chen   +10 more
wiley   +1 more source

Chloroplast DNA methylation in the kelp Saccharina latissima is determined by origin and possibly influenced by cultivation

Evolutionary Applications
DNA cytosine methylation is an important epigenetic mechanism in genomic DNA. In most land plants, it is absent in the chloroplast DNA. We detected methylation in the chloroplast DNA of the kelp Saccharina latissima, a non‐model macroalgal species of ...
Lydia Scheschonk, Anne M. L. Nilsen, Kai Bischof, Alexander Jueterbock   +3 more
doaj   +1 more source

New Insights into 5hmC DNA Modification: Generation, Distribution and Function

Frontiers in Genetics, 2017
Dynamic DNA modifications, such as methylation/demethylation on cytosine, are major epigenetic mechanisms to modulate gene expression in both eukaryotes and prokaryotes.
Dong-Qiao Shi, Iftikhar Ali, Jun Tang, Wei-Cai Yang   +3 more
doaj   +1 more source

Genome-Wide Identification and Comparative Analysis of Cytosine-5 DNA Methyltransferase and Demethylase Families in Wild and Cultivated Peanut

Frontiers in Plant Science, 2016
DNA methylation plays important roles in genome protection, regulation of gene expression and is associated with plants development. Plant DNA methylation pattern was mediated by cytosine-5 DNA methyltransferase and demethylase.
Pengfei Wang, Chao Gao, X. Bian, Shuzhen Zhao, Chuanzhi Zhao, H. Xia, Hui Song, L. Hou, S. Wan, Xingjun Wang   +9 more
semanticscholar   +1 more source

Improved Messenger RNA Stability and Biocompatibility Through Self‐Gelatinizable Nucleic Acids

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Recent advances in the chemical synthesis and modification of messenger RNA (mRNA) have generated growing interest in mRNA‐based therapeutics. However, the inherent instability of mRNA in vivo and during storage remains a major challenge, requiring the development of safe and effective delivery systems.
Takumi Tanifuji, Kosuke Kusamori, Chihiro Tanaka, Hideki Sakai, Shoko Itakura, Makiya Nishikawa   +5 more
wiley   +1 more source

High Yield Branched Puromycin Linker Design Enables Efficient cDNA Display and Chemical Modification of Peptides

Biotechnology and Bioengineering, EarlyView.
A novel puromycin linker for cDNA display was synthesized via SPAAC reaction. Its functionality was confirmed by recovering intact DNA after selection against EpCAM. The system also enabled the verification of bicyclic peptides during display, demonstrating its utility for constructing and screening structurally complex peptide libraries.
Simon Schneider, Melanie Boll, Matthias Eder, Ann‐Christin Eder   +3 more
wiley   +1 more source