Results 1 to 10 of about 885,489 (172)
Chloroplast and mitochondrial DNA editing in plants. [PDF]
Nat Plants, 2021 Abstract Plant organelles, including mitochondria and chloroplasts, contain their own genomes, which encode hundreds of genes essential for respiration and photosynthesis, respectively. Gene editing in plant organelles, an unmet need for plant genetics and biotechnology, has been hampered by the lack of appropriate tools for targeting DNA in ...
Kang BC +9 more
europepmc +5 more sources
Computer simulations explain mutation-induced effects on the DNA editing by adenine base editors. [PDF]
Sci Adv, 2020 Adenine base editors, which were developed by engineering a transfer RNA adenosine deaminase enzyme (TadA) into a DNA editing enzyme (TadA*), enable precise modification of A:T to G⋮C base pairs. Here, we use molecular dynamics simulations to uncover the
Rallapalli KL, Komor AC, Paesani F.
europepmc +3 more sources
From the discovery of DNA to current tools for DNA editing. [PDF]
J Exp Med, 2021 In 1944, the Journal of Experimental Medicine published the groundbreaking discovery that DNA is the molecule holding genetic information (1944. J. Exp. Med.https://doi.org/10.1084/jem.79.2.137). This seminal finding was the genesis of molecular biology and the beginning of an incredible journey to understand, read, and manipulate the genetic code.
Maguin P, Marraffini LA.
europepmc +3 more sources
DNA editing in DNA/RNA hybrids by adenosine deaminases that act on RNA. [PDF]
Nucleic Acids Res, 2017 Adenosine deaminases that act on RNA (ADARs) carry out adenosine (A) to inosine (I) editing reactions with a known requirement for duplex RNA. Here, we show that ADARs also react with DNA/RNA hybrid duplexes.
Zheng Y, Lorenzo C, Beal PA.
europepmc +3 more sources
APOBEC3s: DNA-editing human cytidine deaminases. [PDF]
Protein Sci, 2019 AbstractNucleic acid editing enzymes are essential components of the human immune system that lethally mutate viral pathogens and somatically mutate immunoglobulins. Among these enzymes are cytidine deaminases of the apolipoprotein B mRNA editing enzyme, catalytic polypeptide‐like (APOBEC) super family, each with unique target sequence specificity and ...
Silvas TV, Schiffer CA.
europepmc +5 more sources
Efficient targeted DNA editing and replacement in Chlamydomonas reinhardtii using Cpf1 ribonucleoproteins and single-stranded DNA. [PDF]
Proc Natl Acad Sci U S A, 2017 Significance Our findings establish a method of efficient, targeted genome editing in Chlamydomonas reinhardtii .
Ferenczi A +3 more
europepmc +4 more sources
Catalytic editing properties of DNA polymerases. [PDF]
Proceedings of the National Academy of Sciences, 1995 Enzymatic incorporation of 2',3'-dideoxynucleotides into DNA results in chain termination. We report that 3'-esterified 2'-deoxynucleoside 5'-triphosphates (dNTPs) are false chain-terminator substrates since DNA polymerases, including human immunodeficiency virus reverse transcriptase, can incorporate them into DNA and, subsequently, use this new 3 ...
Bruno Canard +2 more
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Gene Editing with Artificial DNA Scissors [PDF]
Chemistry – A European JournalAbstractArtificial metallo‐nucleases (AMNs) are small molecule DNA cleavage agents, also known as DNA molecular scissors, and represent an important class of chemotherapeutic with high clinical potential. This review provides a primary level of exploration on the concepts key to this area including an introduction to DNA structure, function ...
Alex Gibney, Andrew Kellett
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Editing DNA Methylation in the Mammalian Genome [PDF]
Cell, 2018 Mammalian DNA methylation is a critical epigenetic mechanism orchestrating gene expression networks in many biological processes. However, investigation of the functions of specific methylation events remains challenging. Here, we demonstrate that fusion
Czauderna, Szymon +9 more
core +7 more sources
Prediction of designer-recombinases for DNA editing with generative deep learning. [PDF]
Nat Commun, 2022 Schmitt LT +3 more
europepmc +3 more sources