Results 11 to 20 of about 888,680 (335)
Programmable DNA pyrimidine base editing via engineered uracil-DNA glycosylase
Nature CommunicationsAbstractDNA base editing technologies predominantly utilize engineered deaminases, limiting their ability to edit thymine and guanine directly. In this study, we successfully achieve base editing of both cytidine and thymine by leveraging the translesion DNA synthesis pathway through the engineering of uracil-DNA glycosylase (UNG).
Zongyi Yi +10 more
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In vivo DNA methylation editing in zebrafish
Epigenetics, 2023 The CRISPR/dCas9-based epigenome editing technique has driven much attention. Fused with a catalytic domain from Dnmt or Tet protein, the CRISPR/dCas9-DnmtCD or -TetCD systems possess the targeted DNA methylation editing ability and have established a series of in vitro and in vivo disease models. However, no publication has been reported on zebrafish (
Fang Liang +6 more
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Correction to: ITR-Seq, a next-generation sequencing assay, identifies genome-wide DNA editing sites in vivo following adeno-associated viral vector-mediated genome editing. [PDF]
BMC Genomics, 2020 Breton C +4 more
europepmc +3 more sources
CRISPR/dCas9-Tet1-Mediated DNA Methylation Editing
BIO-PROTOCOLDNA methylation is a key epigenetic mechanism underlying many biological processes, and its aberrant regulation has been tightly associated with various human diseases. Precise manipulation of DNA methylation holds the promise to advance our understanding of this critical mechanism and to develop novel therapeutic methods. Previously, we were only able
Junming Qian, X. Liu
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Mitochondrial DNA editing: Key to the treatment of neurodegenerative diseases. [PDF]
Genes DisHong Y, Song Y, Wang W, Shi J, Chen X.
europepmc +2 more sources
SpCas9- and LbCas12a-Mediated DNA Editing Produce Different Gene Knockout Outcomes in Zebrafish Embryos. [PDF]
Genes (Basel), 2020 Meshalkina DA +4 more
europepmc +2 more sources
CRISPR-Cas9 DNA Base-Editing and Prime-Editing [PDF]
International Journal of Molecular Sciences, 2020 Many genetic diseases and undesirable traits are due to base-pair alterations in genomic DNA. Base-editing, the newest evolution of clustered regularly interspaced short palindromic repeats (CRISPR)-Cas-based technologies, can directly install point-mutations in cellular DNA without inducing a double-strand DNA break (DSB).
Ariel Kantor +2 more
openaire +2 more sources
Grass Meal Acts as a Probiotic in Chicken
Microbiology Research, 2023 Probiotics can act as an alternative to antibiotics in animal feeding, but their use is minimal due to their expensive production. Dry grass is rich with bacteria beneficial for animal feeding and can be used as a probiotic.
Elena S. Bogdanova +7 more
doaj +1 more source
The origin of unwanted editing byproducts in gene editing
Acta Biochimica et Biophysica Sinica, 2022 The rapid development of CRISPR-Cas genome editing tools has greatly changed the way to conduct research and holds tremendous promise for clinical applications.
Yin Jianhang, Hu Jiazhi
doaj +1 more source