Results 21 to 30 of about 888,680 (335)
AID/APOBEC–dependent somatic hypermutation and DNA rearrangements of immunoglobulin and non-immunoglobulin genes [PDF]
Annals of Mechnikov's Institute, 2019 Editing Ig genes by activation induced deaminase (AID) initiates the antibody diversification process in B lymphocytes.In mammalian B cells, this process includes somatic hypermutation (SHM) and class switch recombination (CSR).The activity of AID is ...
T Kolotova +3 more
doaj +2 more sources
Rapid genome editing by CRISPR-Cas9-POLD3 fusion
eLife, 2021 Precision CRISPR gene editing relies on the cellular homology-directed DNA repair (HDR) to introduce custom DNA sequences to target sites. The HDR editing efficiency varies between cell types and genomic sites, and the sources of this variation are ...
Ganna Reint +18 more
doaj +1 more source
Examination of the Cell Cycle Dependence of Cytosine and Adenine Base Editors
Frontiers in Genome Editing, 2022 Base editors (BEs) are genome editing agents that install point mutations with high efficiency and specificity. Due to their reliance on uracil and inosine DNA damage intermediates (rather than double-strand DNA breaks, or DSBs), it has been hypothesized
Cameron A. Burnett +6 more
doaj +1 more source
Chinese Science Bulletin, 2020 CRISPR (clustered regularly interspaced short palindromic repeats)-Cas systems have been extensively used for gene editing of many plant species, including model plants such as Arabidopsis and rice, and major crops such as maize and wheat, as well as woody plants like poplar, bamboo, apple and citrus, etc.
Hongye Gao +3 more
openaire +1 more source
Improving Precise Genome Editing Using Donor DNA/gRNA Hybrid Duplex Generated by Complementary Bases
Biomolecules, 2022 In precise genome editing, site-specific DNA double-strand breaks (DSBs) induced by the CRISPR/Cas9 system are repaired via homology-directed repair (HDR) using exogenous donor DNA templates.
Wataru Aiba +3 more
doaj +1 more source
mitoTev-TALE: a monomeric DNA editing enzyme to reduce mutant mitochondrial DNA levels. [PDF]
EMBO Mol Med, 2018 Pereira CV +6 more
europepmc +2 more sources
New potentials of mitochondrial DNA editing [PDF]
Cell Biology and Toxicology, 2020 Gene modification and editing using mitochondrial DNA (mtDNA) is a promising option to conventional therapy for mitochondrial dysfunction which can result in a wide range of diseases and failure of organ and tissue functions. RNA-free DddA-derived cytosine base editors have been a promising method due to its high target specificity and high product ...
Willian, Wang, Xiangdong, Wang
openaire +2 more sources
Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors. [PDF]
Nat Biotechnol, 2020 Doman JL, Raguram A, Newby GA, Liu DR.
europepmc +2 more sources
Potent CRISPR-Cas9 inhibitors from Staphylococcus genomes. [PDF]
, 2020 Anti-CRISPRs (Acrs) are small proteins that inhibit the RNA-guided DNA targeting activity of CRISPR-Cas enzymes. Encoded by bacteriophage and phage-derived bacterial genes, Acrs prevent CRISPR-mediated inhibition of phage infection and can also block ...
Doudna, Jennifer A +5 more
core +3 more sources
CRISPR Craft: DNA Editing the Reconstructive Ladder. [PDF]
Plast Reconstr Surg, 2019 DeCoster RC +2 more
europepmc +4 more sources