Results 31 to 40 of about 4,873,130 (388)

Comparison of different DNA extraction methods for the molecular study of bark beetles (Coleoptera: Curculionidae, Scolytinae) [PDF]

Journal of Insect Biodiversity and Systematics, 2020
Bark beetles are one of the most important pests in forests. Because of their small size and highly similar morphological characters, molecular approaches may be quite useful for a valid species determination.
sudabe Amini, Jamasb Nozari, Reza Hosseini, Rassol Rahati   +3 more
doaj  

Comparison of DNA extraction methods for molecular identification of pathogenic Leptospira in the urine samples

Health Science Journal of Indonesia, 2020
Latar belakang: Leptospirosis merupakan zoonosis penting di dunia, yang masih sering terjadi salah diagnosis. Deteksi laboratorium Leptospira menjadi tantangan karena bakterimea cukup singkat untuk dideteksi molekuler, namun antibodi juga muncul sangat ...
Farida Dwi Handayani, Rahmi Ayu Wijayaningsih, Muhammad Hussein Gasem, Tri Wibawa   +3 more
doaj   +1 more source

Studies on the Rapid and Simple DNA Extraction Method, Antibacterial Activity and Enzyme Activity Involved in Plant Biomass Conversion by Cookeina sulcipes and C. tricholoma (Cup Fungi)

Journal of Pure and Applied Microbiology, 2022
Cookeina sulcipes and C. tricholoma are a cup fungi (Ascomycota) collected in Saraburi, Thailand. The fungi have been isolated, cultured and confirmed as respective species. For morphology, both Cookeina sp.
Narumon Tangthirasunun, Supattra Poeaim
doaj   +1 more source

A minimally destructive protocol for DNA extraction from ancient teeth

bioRxiv, 2020
Ancient DNA sampling methods—although optimized for efficient DNA extraction—are destructive, relying on drilling or cutting and powdering (parts of) bones and teeth. As the field of ancient DNA has grown, so have concerns about the impact of destructive
Éadaoin Harney, O. Cheronet, D. Fernandes, K. Sirak, Matthew Mah, Rebecca Bernardos, Nicole Adamski, Nasreen Broomandkhoshbacht, Kimberly Callan, A. M. Lawson, Jonas Oppenheimer, Kristin Stewardson, Fatma Zalzala, A. Anders, F. Candilio, M. Constantinescu, A. Coppa, Ion Ciobanu, János Dani, Zsolt Gallina, F. Genchi, Emese Gyöngyvér Nagy, T. Hajdu, Magdolna Hellebrandt, Antónia Horváth, Á. Király, K. Kiss, Barbara Kolozsi, Péter F. Kovács, K. Köhler, M. Lucci, I. Pap, S. Popovici, P. Raczky, Angela Simalcsik, Tamás Szeniczey, S. Vasilyev, Cristian Virag, N. Rohland, D. Reich, R. Pinhasi   +40 more
semanticscholar   +1 more source

An efficient method for DNA extraction from Cladosporioid fungi [PDF]

, 2010
We developed an efficient method for DNA extraction from Cladosporioid fungi, which are important fungal plant pathogens. The cell wall of Cladosporioid fungi is often melanized, which makes it difficult to extract DNA from their cells.
Abd-Elsalam, K.A., Bahkali, A.H., Moslem, M.A., Wit, P.J.G.M., de   +3 more
core   +2 more sources

A Rapid and Simple Method for DNA Preparation of Magnaporthe oryzae from Single Rice Blast Lesions for PCR-Based Molecular Analysis [PDF]

The Plant Pathology Journal, 2022
Rice blast is one of the most destructive diseases of rice worldwide, and the causative agent is the filamentous ascomycete Magnaporthe oryzae. With the successful cloning of more and more avirulence genes from M.
Liying Dong, Shufang Liu, Jing Li, Didier Tharreau, Pei Liu, Dayun Tao, Qinzhong Yang   +6 more
doaj   +1 more source

SILEX: a fast and inexpensive high-quality DNA extraction method suitable for multiple sequencing platforms and recalcitrant plant species

Plant Methods, 2020
Background The use of sequencing and genotyping platforms has undergone dramatic improvements, enabling the generation of a wealth of genomic information.
S. Vilanova, D. Alonso, P. Gramazio, M. Plazas, Edgar García-Fortea, P. Ferrante, M. Schmidt, M. Díez, B. Usadel, G. Giuliano, J. Prohens   +10 more
semanticscholar   +1 more source

Optimization of DNA extraction from human urinary samples for mycobiome community profiling. [PDF]

, 2019
IntroductionRecent data suggest the urinary tract hosts a microbial community of varying composition, even in the absence of infection. Culture-independent methodologies, such as next-generation sequencing of conserved ribosomal DNA sequences, provide an
Ackerman, A Lenore, Ackerman, James E, Anger, Jennifer Tash, Freeman, Michael R, Khalique, Muhammad Umair, Kim, Jayoung, NIH Multidisciplinary Approach to the Study of Chronic Pelvic Pain (MAPP), Tang, Jie, Underhill, David M   +8 more
core   +3 more sources

Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material.

BioTechniques, 2013
Procedures utilizing Chelex 100 chelating resin have been developed for extracting DNA from forensic-type samples for use with the PCR. The procedures are simple, rapid, involve no organic solvents and do not require multiple tube transfers for most ...
P. Walsh, D. A. Metzger, Mohammed Taha
semanticscholar   +1 more source

Application of a multiplex polymerase chain reaction (mPCR) assay to detect fraud by substitution of bovine meat cuts with water buffalo meat in Northern Brazil

CyTA - Journal of Food, 2019
The adulteration of meat products can affect the confidence of consumers and the market, leading to negative impacts on the economy. Accordingly, product authenticity has become an important issue in modern society. Therefore, our study aimed to optimize
Vanderson Vasconcelos Dantas, Gabrielle Virginia Ferreira Cardoso, Wanessa Shuelen Costa Araújo, Andrey Carlos do Sacramento de Oliveira, Andreia Silva da Silva, Josyane Brasil da Silva, Silvia Cristina da Silva Pedroso, Talita Bandeira Roos, Carina Martins de Moraes, Lúcia de Fátima Henriques Lourenço   +9 more
doaj   +1 more source