Results 31 to 40 of about 4,748,303 (367)
A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
Nucleic Acids Research, 1979 A procedure for extracting plasmid DNA from bacterial cells is described. The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction ...
H. Birnboim, J. Doly
semanticscholar +1 more source
Rapid diagnostic tests for molecular surveillance of Plasmodium falciparum malaria -assessment of DNA extraction methods and field applicability [PDF]
, 2013 Background: The need for new malaria surveillance tools and strategies is critical, given improved global malaria control and regional elimination efforts.
Abdullah S Ali +26 more
core +2 more sources
METHODOLOGICAL ASPECTS OF REAL-TIME PCR USAGE IN ACETOBACTER DETECTION [PDF]
Journal of Engineering Science (Chişinău), 2020 Acetic acid bacteria occur in sugar and alcoholised, slightly acid niches such as flowers, fruits, beer, wine, cider, vinegar, souring fruit juices and honey.
BOISTEAN, Alina +4 more
doaj +1 more source
Optimized protocol for DNA extraction from ancient skeletal remains using Chelex-100 [PDF]
, 2021 PCR-based analysis of skeletonized human remains is a common aspect in both forensic human identification as well as Ancient DNA research. In this, both areas not merely utilize very similar methodology, but also share the same problems regarding quantity and quality of recovered DNA and presence of inhibitory substances in samples from excavated ...
arxiv +1 more source
Journal of Forest Science, 2010 The objective of this work was to apply selected genetic markers to verify the genetic purity of bog pine (Pinus uncinata subsp. uliginosa) individuals from the Borkovicka blata population in order to initiate in vitro cultures from them.
H. Vejsadová, M. Lukášová
doaj +1 more source
mSystems, 2020 Method choice throughout the workflow of a microbiome study, from sample collection to DNA extraction and sequencing procedures, can greatly affect results.
Q. R. Ducarmon +4 more
semanticscholar +1 more source
Point of Care Diagnosis of Multiple Schistosome Parasites: Species-specific DNA Detection in Urine by Loop-mediated Isothermal Amplification (LAMP) [PDF]
, 2017 Schistosomes are easily transmitted and high chance of repeat infection, so if control strategies based on targeted mass drug administration (MDA) are to succeed it is essential to have a test that is sensitive, accurate and simple to use.
Anyan, William K. +6 more
core +2 more sources
Effects of sampling strategy and DNA extraction on human skin microbiome investigations
Scientific Reports, 2019 The human skin is colonized by a wide array of microorganisms playing a role in skin disorders. Studying the skin microbiome provides unique obstacles such as low microbial biomass.
R. Bjerre +5 more
semanticscholar +1 more source
CyTA - Journal of Food, 2019 The adulteration of meat products can affect the confidence of consumers and the market, leading to negative impacts on the economy. Accordingly, product authenticity has become an important issue in modern society. Therefore, our study aimed to optimize
Vanderson Vasconcelos Dantas +9 more
doaj +1 more source
Studying DNA translocation in nanocapillaries using single molecule fluorescence [PDF]
Appl. Phys. Lett. vol.101, 223704 (2012), 2013 We demonstrate simultaneous measurements of DNA translocation into glass nanopores using ionic current detection and fluorescent imaging. We verify the correspondence between the passage of a single DNA molecule through the nanopore and the accompanying characteristic ionic current blockage.
arxiv +1 more source