Results 31 to 40 of about 846,282 (296)

Optimization of DNA Extraction Methods in Fresh Meat (Rat and Chicken Meat) based on Incubation Time

open access: yesIndonesian Journal of Halal Research, 2023
DNA (deoxyribonucleic acid) extraction method is the process of separating DNA from the sample. In this process, the DNA obtained must be protected from contamination by RNA, carbohydrates, lipids, and proteins.
Hadi Sunaryo   +5 more
doaj   +1 more source

A Rapid Bacteriophage DNA Extraction Method [PDF]

open access: yesMethods and Protocols, 2018
Bacteriophages (phages) are intensely investigated as non-antibiotic alternatives to circumvent antibiotic resistance development as well as last resort therapeutic options against antibiotic resistant bacteria. As part of gaining a better understanding of phages and to determine if phages harbor putative virulence factors, whole genome sequencing is ...
Džiuginta Jakočiūnė, Arshnee Moodley
openaire   +5 more sources

Circulating histones as clinical biomarkers in critically ill conditions

open access: yesFEBS Letters, EarlyView.
Circulating histones are emerging as promising biomarkers in critical illness due to their diagnostic, prognostic, and therapeutic potential. Detection methods such as ELISA and mass spectrometry provide reliable approaches for quantifying histone levels in plasma samples.
José Luis García‐Gimenez   +17 more
wiley   +1 more source

Molecular characterization of rice (Oryza sativa L.) genotypes for drought tolerance using two SSR markers

open access: yesElectronic Journal of Plant Breeding, 2017
Plant growth as well as productivity of rice (Oryza sativa L.), the major cereal and staple food of millions, is significantly affected by numerous biotic and abiotic stresses.
Shaheen A. Salam   +3 more
doaj   +1 more source

The epithelial barrier theory proposes a comprehensive explanation for the origins of allergic and other chronic noncommunicable diseases

open access: yesFEBS Letters, EarlyView.
Exposure to common noxious agents (1), including allergens, pollutants, and micro‐nanoplastics, can cause epithelial barrier damage (2) in our body's protective linings. This may trigger an immune response to our microbiome (3). The epithelial barrier theory explains how this process can lead to chronic noncommunicable diseases (4) affecting organs ...
Can Zeyneloglu   +17 more
wiley   +1 more source

Investigation of Bm86 gene analog sequence in some Iranian populations of Hyalomma anatolicum species [PDF]

open access: yesIranian Journal of Animal Biosystematics, 2006
Ticks are an important problem among zoonotic diseases. Main veterinary species of hard ticks are: Hyalomma sp. Boophilus sp and Rhipicephalus sp. Among them Hyalomma sp. have widespread distribution in Iran and Middle East.
S. Abdollahi   +2 more
doaj   +1 more source

Optimization of proteinase K incubation protocol duration during DNA extraction from oral squamous cell carcinoma FFPE samples

open access: yesDental Journal, 2023
Background: Formalin-fixed paraffin-embedded (FFPE) specimen archives are a valuable source of sample material for molecular biological analysis. However, the DNA isolated from FFPE samples is usually low in concentration and fragmented.
Asti Meizarini   +3 more
doaj   +1 more source

From omics to AI—mapping the pathogenic pathways in type 2 diabetes

open access: yesFEBS Letters, EarlyView.
Integrating multi‐omics data with AI‐based modelling (unsupervised and supervised machine learning) identify optimal patient clusters, informing AI‐driven accurate risk stratification. Digital twins simulate individual trajectories in real time, guiding precision medicine by matching patients to targeted therapies.
Siobhán O'Sullivan   +2 more
wiley   +1 more source

Thermostable neutral metalloprotease from Geobacillus sp. EA1 does not share thermolysin's preference for substrates with leucine at the P1′ position

open access: yesFEBS Letters, EarlyView.
Knowing how proteases recognise preferred substrates facilitates matching proteases to applications. The S1′ pocket of protease EA1 directs cleavage to the N‐terminal side of hydrophobic residues, particularly leucine. The S1′ pocket of thermolysin differs from EA's at only one position (leucine in place of phenylalanine), which decreases cleavage ...
Grant R. Broomfield   +3 more
wiley   +1 more source

Comparative Study of Extracted Nucleic Acid from Escherichia coli by Two Methods Phenol-chloroform and Extraction Using Magnetic Nanoparticle

open access: yesBihdād, 2020
Background: The most important researches in molecular and genetic engineering fields are the finding of optimal methods for extraction of the genomic content of microorganisms and cells using them, can achieve the most amount and purity with the least ...
Ala Amiri   +3 more
doaj  

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