Results 31 to 40 of about 900,113 (174)
Background: The most important researches in molecular and genetic engineering fields are the finding of optimal methods for extraction of the genomic content of microorganisms and cells using them, can achieve the most amount and purity with the least ...
Ala Amiri+3 more
doaj
Simplified DNA Extraction Methods for Sexing Chick Embryos
We investigated the efficiency of DNA extraction and the accuracy of polymerase chain reaction- (PCR-) based sexing of DNA that was obtained from adult chickens and early chicken embryos. DNA samples were prepared using water-, ammonium-, and proteinase-
Takeo Minematsu+4 more
doaj +1 more source
Inferring coarse-grain histone-DNA interaction potentials from high-resolution structures of the nucleosome [PDF]
The histone-DNA interaction in the nucleosome is a fundamental mechanism of genomic compaction and regulation, which remains largely unkown despite a growing structural knowledge of the complex. Here, we propose a framework for the extraction of a nanoscale histone-DNA force-field from a collection of high-resolution structures, which may be adapted to
arxiv +1 more source
Nucleation and growth in one dimension, part II: Application to DNA replication kinetics [PDF]
Inspired by recent experiments on DNA replication, we apply a one-dimensional nucleation-and-growth model to DNA-replication kinetics, focusing on how to extract the time-dependent nucleation rate I(t) and growth speed v from data. We discuss generic experimental problems, namely spatial inhomogeneity, measurement noise, and finite-size effects.
arxiv +1 more source
The objective of this study was to develop a simple and quick DNA extraction procedure for rapid diagnosis of sex of chicken and its embryos using multiplex polymerase chain reaction (PCR).
Santosh Haunshi+4 more
doaj +1 more source
Comparing Machine Learning Algorithms with or without Feature Extraction for DNA Classification [PDF]
The classification of DNA sequences is a key research area in bioinformatics as it enables researchers to conduct genomic analysis and detect possible diseases. In this paper, three state-of-the-art algorithms, namely Convolutional Neural Networks, Deep Neural Networks, and N-gram Probabilistic Models, are used for the task of DNA classification ...
arxiv
The Fluctuations of Blocked Ionic Current Reveal the Instantaneous Statuses of DNA in Graphene Nanopore [PDF]
Extracting the sequence information of DNA from the blocked ionic current is the crucial step of the ionic current-based nanopore sequencing approaches. The thinnest graphene nanopore, which contained only one layer of carbon atoms, potentially has ultra-high DNA sequencing sensitivity.
arxiv +1 more source
Indonesia is a country with high variability of microorganisms, including bacteria, yeast, and fungi. Yeast isolates could be isolated from the honeycomb of Apis dorsata. Molecular approaches were used to identify yeast using ribosomal DNA gene sequences,
Chumaidatul Choiriyah, S.Si+4 more
doaj +1 more source
Using microbiome information to understand and improve animal performance
For many years, microbiology was based on culture-dependent methods to study microorganisms; however, the latest technologies are culture-independent and their development has generated large amounts of new data, allowing researchers to understand the ...
Jeferson Menezes Lourenco+1 more
doaj +1 more source
DNA nano-mechanics: how proteins deform the double helix [PDF]
It is a standard exercise in mechanical engineering to infer the external forces and torques on a body from its static shape and known elastic properties. Here we apply this kind of analysis to distorted double-helical DNA in complexes with proteins. We extract the local mean forces and torques acting on each base-pair of bound DNA from high-resolution
arxiv +1 more source