Insights into PI3K/AKT signaling in B cell development and chronic lymphocytic leukemia
FEBS Letters, EarlyView.This Review explores how the phosphoinositide 3‐kinase and protein kinase B pathway shapes B cell development and drives chronic lymphocytic leukemia, a common blood cancer. It examines how signaling levels affect disease progression, addresses treatment challenges, and introduces novel experimental strategies to improve therapies and patient outcomes.
Maike Buchner
wiley +1 more source
Study of the DNA Extraction from the Nail by Spin Column-based Nucleic Acid Purification
Journal of Forensic Science and MedicineBackground: Nails are one of the objects that are more durable than other witness objects. This study was interested in whether the Spin column-based nucleic acid purification technique could extract DNA from nails to give satisfactory results.
Kanchana Sujirachato +5 more
doaj +1 more source
ISOLATION AND IDENTIFICATION OF ALOCALSTRAIN OF PROBIOTIC BACTERIAL ACTOBACILLUS PLANTARUM AND STUDIED THE TOLERANCE ABILITY FOR DIFFERENT LEVELS OF pH [PDF]
Basrah Journal of Veterinary Research, 2016 Lactobacillus plantarum IRQ12 was isolated from local pickle producing from Cucumis sativus submerged in 5% salt solution for one week far from sun light, selective media was used for isolation and activation process which was MRS broth and MRS agar ...
Sinan J. Abdul-Abbas +3 more
doaj +1 more source
Use of FFPE-derived DNA in next generation sequencing: DNA extraction methods
bioRxiv, 2019 Archival tissues represent a rich resource for clinical genomic studies, particularly when coupled with comprehensive medical records. Use of these in NGS is a priority.
S. McDonough +7 more
semanticscholar +1 more source
Making tau amyloid models in vitro: a crucial and underestimated challenge
FEBS Letters, EarlyView.This review highlights the challenges of producing in vitro amyloid assemblies of the tau protein. We review how accurately the existing protocols mimic tau deposits found in the brain of patients affected with tauopathies. We discuss the important properties that should be considered when forming amyloids and the benchmarks that should be used to ...
Julien Broc, Clara Piersson, Yann Fichou
wiley +1 more source
Rapid detection protocol ofFusarium oxysporum f. sp.cubense race 1 inbanana
Biotecnología Vegetal, 2021 Fusarium wilt of banana is a typical vascular wilt disease caused byFusarium oxysporum f.sp.cubense. The genetic improvement program of the crop needs tools that allow the quickdetection of fungal phytopathogen.
María I. Oloriz +4 more
doaj
Determining the optimal method for DNA isolation from fruit jams
Czech Journal of Food Sciences, 2018 DNA extraction is a crucial step in PCR analysis especially when analysing food samples that can be degraded and can potentially contain PCR-inhibiting substances.
Tereza SOVOVÁ +2 more
doaj +1 more source
Estimating bacterial DNA synthesis from [3H] thymidine incorporation : discrepancies among macromolecular extraction procedures [PDF]
, 1991 The estimation of bacterial DNA synthesis in trophic studies with [3H] thymidine requires quantitative extraction of labeled DNA. To determine the DNA contribution to total macromolecular labeling in a eutrophic ecosystem, we tested three extraction ...
Bouvy, Marc, Torréton, Jean-Pascal
core +1 more source
A stepwise emergence of evolution in the RNA world
FEBS Letters, EarlyView.How did biological evolution emerge from chemical reactions? This perspective proposes a gradual scenario of self‐organization among RNA molecules, where catalytic feedback on random mixtures plays the central role. Short oligomers cross‐ligate, and self‐assembly enables heritable variations. An event of template‐externalization marks the transition to
Philippe Nghe
wiley +1 more source
Optimization of DNA Extraction Methods in Fresh Meat (Rat and Chicken Meat) based on Incubation Time
Indonesian Journal of Halal Research, 2023 DNA (deoxyribonucleic acid) extraction method is the process of separating DNA from the sample. In this process, the DNA obtained must be protected from contamination by RNA, carbohydrates, lipids, and proteins.
Hadi Sunaryo +5 more
doaj +1 more source