Results 81 to 90 of about 4,748,303 (367)

Inter-DNA Attraction Mediated by Divalent Counterions

, 2007
Can nonspecifically bound divalent counterions induce attraction between DNA strands? Here, we present experimental evidence demonstrating attraction between short DNA strands mediated by Mg2 ions.
Andresen, Kurt, Kwok, Lisa W., Lamb, Jessica S., Park, Hye Yoon, Pollack, Lois, Qiu, Xiangyun   +5 more
core   +1 more source

Study of the DNA Extraction from the Nail by Spin Column-based Nucleic Acid Purification

Journal of Forensic Science and Medicine
Background: Nails are one of the objects that are more durable than other witness objects. This study was interested in whether the Spin column-based nucleic acid purification technique could extract DNA from nails to give satisfactory results.
Kanchana Sujirachato, Piya Wongyanin, Pilaiwan Ramadjai, Alisa Ladsuk, Kingkan Pokkasap, Wisarn Worasuwannarak   +5 more
doaj   +1 more source

Can non-destructive DNA extraction of bulk invertebrate samples be used for metabarcoding?

PeerJ, 2018
Background High throughput DNA sequencing of bulk invertebrate samples or metabarcoding is becoming increasingly used to provide profiles of biological communities for environmental monitoring.
M. Carew, R. Coleman, A. Hoffmann
semanticscholar   +1 more source

miRNA‐29 regulates epidermal and mesenchymal functions in skin repair

FEBS Letters, EarlyView.
miRNA‐29 inhibits cell‐to‐cell and cell‐to‐matrix adhesion by silencing mRNA targets. Adhesion is controlled by complex interactions between many types of molecules coded by mRNAs. This is crucial for keeping together the layers of the skin and for regenerating the skin after wounding.
Lalitha Thiagarajan, Rosa Sanchez‐Alvarez, Chiho Kambara, Poojitha Rajasekar, Yuluang Wang, François Halloy, Jonathan Hall, Hans‐Jürgen Stark, Iris Martin, Petra Boukamp, Svitlana Kurinna   +10 more
wiley   +1 more source

Attempted DNA extraction from a Rancho La Brea Columbian mammoth (Mammuthus columbi): prospects for ancient DNA from asphalt deposits. [PDF]

, 2014
Fossil-bearing asphalt deposits are an understudied and potentially significant source of ancient DNA. Previous attempts to extract DNA from skeletons preserved at the Rancho La Brea tar pits in Los Angeles, California, have proven unsuccessful, but it ...
Farrell, Aisling B, Gold, David A, Harris, John M, Jacobs, David K, Robinson, Jacqueline, Thalmann, Olaf   +5 more
core   +1 more source

Using microbiome information to understand and improve animal performance

Italian Journal of Animal Science, 2022
For many years, microbiology was based on culture-dependent methods to study microorganisms; however, the latest technologies are culture-independent and their development has generated large amounts of new data, allowing researchers to understand the ...
Jeferson Menezes Lourenco, Christina Breanne Welch   +1 more
doaj   +1 more source

A cellular system to study responses to a collision between the transcription complex and a protein‐bound nick in the DNA template

FEBS Letters, EarlyView.
We present the cellular transcription‐coupled Flp‐nick system allowing the introduction of a Top1‐mimicking cleavage complex (Flpcc) at a Flp recognition target site within a controllable LacZ gene. LacZ transcription leads to the collision of RNA polymerase II (RNAPII) with Flpcc, and this causes RNAPII stalling, ubiquitination, and degradation.
Petra Herring, Morten Roedgaard, Camilla Myrup Holst, Helene Christensen, Birgitta R. Knudsen, Lotte Bjergbaek, Anni Hangaard Andersen   +6 more
wiley   +1 more source

Following basal stem rot in young oil palm plantings [PDF]

, 2005
The PCR primer GanET has previously been shown to be suitable for the specific amplification of DNA from Ganoderma boninense. A DNA extraction and PCR method has been developed that allows for the amplification of the G.
CA Levesque, CA Pilotti, DM Hillis, DS Hibbet, FR Sanderson, FR Sanderson, FR Sanderson, G. Panchal, J-M Moncalvo, J-M Moncalvo, J-M Moncalvo, J-M Moncalvo, OF Cubero, P. D. Bridge, PD Bridge, PD Bridge, PR Mills, TJ White   +17 more
core   +1 more source

Protonophore activity of short‐chain fatty acids induces their intracellular accumulation and acidification

FEBS Letters, EarlyView.
The protonated form of butyrate, as well as other short‐chain fatty acids (SCFAs), is membrane permeable. In acidic extracellular environments, this can lead to intracellular accumulation of SCFAs and cytosolic acidification. This phenomenon will be particularly relevant in acidic environments such as the large intestine or tumor microenvironments ...
Muwei Jiang, Frans Bianchi, Geert van den Bogaart   +2 more
wiley   +1 more source

Efficient DNA isolation from moroccan arar tree [Tetraclinis articulata (Vahl) Masters] leaves and optimization of the rapd-pcr molecular technique [PDF]

, 2010
Efficient DNA isolation from Moroccan Arar tree [Tetraclinis articulata (Vahl) Masters] leaves and optimization of the RAPD-PCR molecular technique. Molecular genetic analysis of Arar tree [Tetraclinis articulata (Vahl) Masters] is often limited by the
Abourouh, Mohamed, Bakkali Yakhlefb, Salaheddine, Guenoun, Imane, Hamamouch, Noureddine, kerdouh, Benaîssa   +4 more
core   +2 more sources