Structural and mechanistic insights into the DNA glycosylase AAG-mediated base excision in nucleosome [PDF]
Cell Discovery, 2023 The engagement of a DNA glycosylase with a damaged DNA base marks the initiation of base excision repair. Nucleosome-based packaging of eukaryotic genome obstructs DNA accessibility, and how DNA glycosylases locate the substrate site on nucleosomes is ...
Lvqin Zheng, Bin Tsai, Ning Gao
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A thermophilic 8-oxoguanine DNA glycosylase from Thermococcus barophiluss Ch5 is a new member of AGOG DNA glycosylase family [PDF]
Acta Biochimica et Biophysica Sinica, 2022 8-Oxoguanine (8oxoG) in DNA is a major oxidized base that poses a severe threat to genome stability. To counteract the mutagenic effect generated by 8oxoG in DNA, cells have evolved 8oxoG DNA glycosylase (OGG) that can excise this oxidized base from DNA.
Wang Lei, Jiang Donghao, Zhang Likui
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Mismatch uracil DNA glycosylase (Mug) is maintained in the Corynebacterium pseudotuberculosis genome and exhibits affinity for uracil but not other types of damage [PDF]
Genetics and Molecular BiologyThe genome of Corynebacterium pseudotuberculosis, etiologic agent of Caseous Lymphadenitis (CLA), was sequenced to comprehend its genetics, pathogenicity, and virulence mechanisms due to its economic importance.
Bruno Carvalho Resende +9 more
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Nature Communications, 2020 DNA glycosylases are lesion-specific enzymes that recognize specific nucleobase damages and catalyze their excision through cleavage of the glycosidic bond.
Uddhav K. Shigdel +6 more
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A novel uracil-DNA glycosylase family related to the helix-hairpin-helix DNA glycosylase superfamily [PDF]
Nucleic Acids Research, 2003 Cytosine bases can be deaminated spontaneously to uracil, causing DNA damage. Uracil-DNA glycosylase (UDG), a ubiquitous uracil-excising enzyme found in bacteria and eukaryotes, is one of the enzymes that repair this kind of DNA damage. To date, no UDG-coding gene has been identified in Methanococcus jannaschii, although its entire genome was ...
Ji Hyung Chung
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Structure of a DNA Glycosylase Bound to a Nicked T:G Mismatch-Containing DNA [PDF]
MoleculesMismatched T:G base pairs can arise during de novo replication as well as base excision repair (BER). In particular, the action of the gap-filling polymerase β (Polβ) can generate a T:G pair as well as a nick in the DNA backbone.
Hala Ouzon-Shubeita +3 more
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Epigenetics, 2017 DNA methylation is a crucial epigenetic mark associated to gene silencing, and its targeted removal is a major goal of epigenetic editing. In animal cells, DNA demethylation involves iterative 5mC oxidation by TET enzymes followed by replication ...
Jara Teresa Parrilla-Doblas +2 more
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Uracil-DNA Glycosylase UNG Promotes Tet-mediated DNA Demethylation* [PDF]
Journal of Biological Chemistry, 2015 In mammals, active DNA demethylation involves oxidation of 5-methylcytosine (5mC) into 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) by Tet dioxygenases and excision of these two oxidized bases by thymine DNA glycosylase (TDG).
Jian-Huang Xue +9 more
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Programmable DNA pyrimidine base editing via engineered uracil-DNA glycosylase [PDF]
Nature CommunicationsDNA base editing technologies predominantly utilize engineered deaminases, limiting their ability to edit thymine and guanine directly. In this study, we successfully achieve base editing of both cytidine and thymine by leveraging the translesion DNA ...
Zongyi Yi +10 more
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