Results 21 to 30 of about 19,626 (172)

CID12261165, a flavonoid compound as antibacterial agents against quinolone-resistant Staphylococcus aureus

Scientific Reports, 2023
Flavonoids are plant-produced secondary metabolites that are found ubiquitously. We have previously reported that apigenin, a class of flavonoid, has unique antimicrobial activity against Staphylococcus aureus (S.
Yuh Morimoto, Yoshifumi Aiba, Kazuhiko Miyanaga, Tomomi Hishinuma, Longzhu Cui, Tadashi Baba, Keiichi Hiramatsu   +6 more
doaj   +1 more source

Potent DNA gyrase inhibitors bind asymmetrically to their target using symmetrical bifurcated halogen bonds

Nature Communications, 2021
The mechanism of DNA gyrase inhibitor stabilization of single-strand DNA cleavage breaks by DNA gyrase has been hypothetical. Here, the authors show experimental evidence of the mechanism using a library of inhibitors with improved binding and employ ...
Anja Kolarič, Thomas Germe, Martina Hrast, Clare E. M. Stevenson, David M. Lawson, Nicolas P. Burton, Judit Vörös, Anthony Maxwell, Nikola Minovski, Marko Anderluh   +9 more
doaj   +1 more source

DNA supercoiling in gyrase mutants [PDF]

Journal of Bacteriology, 1984
Nucleoids isolated from Escherichia coli strains carrying temperature-sensitive gyrA or gyrB mutations were examined by sedimentation in ethidium bromide-containing sucrose density gradients. A shift to restrictive temperature resulted in nucleoid DNA relaxation in all of the mutant strains.
T R, Steck, G J, Pruss, S H, Manes, L, Burg, K, Drlica   +4 more
openaire   +2 more sources

Bacterial Argonaute Proteins Aid Cell Division in the Presence of Topoisomerase Inhibitors in Escherichia coli

Microbiology Spectrum, 2023
Prokaryotic Argonaute (pAgo) proteins are guide-dependent nucleases that function in host defense against invaders. Recently, it was shown that TtAgo from Thermus thermophilus also participates in the completion of DNA replication by decatenating ...
Anna Olina, Aleksei Agapov, Denis Yudin, Dmitry Sutormin, Alina Galivondzhyan, Anton Kuzmenko, Konstantin Severinov, Alexei A. Aravin, Andrey Kulbachinskiy   +8 more
doaj   +1 more source

CryoEM structures of open dimers of gyrase A in complex with DNA illuminate mechanism of strand passage

eLife, 2018
Gyrase is a unique type IIA topoisomerase that uses ATP hydrolysis to maintain the negatively supercoiled state of bacterial DNA. In order to perform its function, gyrase undergoes a sequence of conformational changes that consist of concerted gate ...
Katarzyna M Soczek, Tim Grant, Peter B Rosenthal, Alfonso Mondragón   +3 more
doaj   +1 more source

Structural insights into the quinolone resistance mechanism of Mycobacterium tuberculosis DNA gyrase. [PDF]

PLoS ONE, 2010
Mycobacterium tuberculosis DNA gyrase, an indispensable nanomachine involved in the regulation of DNA topology, is the only type II topoisomerase present in this organism and is hence the sole target for quinolone action, a crucial drug active against ...
Jérémie Piton, Stéphanie Petrella, Marc Delarue, Gwénaëlle André-Leroux, Vincent Jarlier, Alexandra Aubry, Claudine Mayer   +6 more
doaj   +1 more source

PprA Protein Is Involved in Chromosome Segregation via Its Physical and Functional Interaction with DNA Gyrase in Irradiated Deinococcus radiodurans Bacteria

mSphere, 2016
PprA, a radiation-induced Deinococcus-specific protein, was previously shown to be required for cell survival and accurate chromosome segregation after exposure to ionizing radiation. Here, we used an in vivo approach to determine, by shotgun proteomics,
Alice Devigne, Philippe Guérin, Johnny Lisboa, Sophie Quevillon-Cheruel, Jean Armengaud, Suzanne Sommer, Claire Bouthier de la Tour, Pascale Servant   +7 more
doaj   +1 more source

Design and synthesis of new 3-((7-chloroquinolin-4-yl)amino)thiazolidin-4-one analogs as Mycobacterium tuberculosis DNA gyrase inhibitors

Future Journal of Pharmaceutical Sciences, 2021
Background Tuberculosis is evidently a major health threat among human populations worldwide. The current study presents the synthesis of new 3-((7-chloroquinolin-4-yl)amino)thiazolidin-4-one analogs (4a–o) as potential Mycobacterium tuberculosis DNA ...
Preeti S. Salve, Prajakta Parchure, Lisel Araujo, Rohini S. Kavalapure, Sunil S. Jalalpure, Dharmarajan Sriram, Vagolu Siva Krishna, Madhu Rekha Estharla, Shankar G. Alegaon   +8 more
doaj   +1 more source

Mechanism of Inhibition of DNA Gyrase by ES‐1273, a Novel DNA Gyrase Inhibitor [PDF]

Microbiology and Immunology, 2007
AbstractWe investigated the mode of action of ES‐1273, a novel DNA gyrase inhibitor obtained by optimization of ES‐0615, which was found by screening our chemical library using anucleate cell blue assay. ES‐1273 exhibited the same antibacterial activity against S. aureus strains with amino acid change(s) conferring quinolone‐ and coumarin‐resistance as
Yoshihiro, Oyamada, Jun-ichi, Yamagishi, Takahiro, Kihara, Hiroaki, Yoshida, Masaaki, Wachi, Hideaki, Ito   +5 more
openaire   +2 more sources

Characterization of the Interaction between DNA Gyrase Inhibitor and DNA Gyrase of Escherichia coli [PDF]

Journal of Biological Chemistry, 2002
Escherichia coli DNA gyrase is comprised of two subunits, GyrA and GyrB. Previous studies have shown that GyrI, a regulatory factor of DNA gyrase activity, inhibits the supercoiling activity of DNA gyrase and that both overexpression and antisense expression of the gyrI gene suppress cell proliferation.
Akira, Nakanishi, Shinobu, Imajoh-Ohmi, Fumio, Hanaoka   +2 more
openaire   +2 more sources