DNA isolation protocol effects on nuclear DNA analysis by microarrays, droplet digital PCR, and whole genome sequencing, and on mitochondrial DNA copy number estimation. [PDF]
PLoS ONE, 2017 Potential bias introduced during DNA isolation is inadequately explored, although it could have significant impact on downstream analysis. To investigate this in human brain, we isolated DNA from cerebellum and frontal cortex using spin columns under ...
Elizabeth Nacheva +12 more
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A simple and cost-effective method for DNA extraction suitable for PCR in “sucupira branca”
Bioscience Journal, 2021 “Sucupira branca” is a plant found in the Brazilian Cerrado and is adapted to low fertility soils, and its fruit extract has anti-inflammatory, healing, antiulcerogenic, antimicrobial, cercaricidal, leishmanicidal and antioxidant activities. Furthermore,
Jaílson do Nascimento Silva +6 more
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Optimized method for dna extraction and PCR amplification in aroeira tree
Bioscience Journal, 2023 Molecular markers are important tools in the characterization of plant genetic diversity and can provide support for conservation strategies for endangered populations.
Jailson do Nascimento Silva +8 more
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Gelatin-coated magnetic nanoparticles-based DNA isolation method: A comparison with commercial DNA isolation kits from whole blood [PDF]
Journal of Bioscience and Applied Research, 2019 DNA isolation is the first step for most molecular tests, thus indicating the importance of using a reliable and suitable method for this procedure. The use of magnetic nanoparticles for blood DNA isolation offers several advantages including subjecting ...
Kelly Levano +3 more
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Comparison of DNA isolation methods from mammalian sperm cells and development of a new protocol
Medicine Science, 2021 Sperm DNA tightly packed with protamines makes the DNA isolation procedure from sperm cells long and laborious. Cell lysis is also a challenging step because of the disulfide bonds-rich membranes of the sperm cells.
Hatice Sevim Nalkiran +4 more
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The features of diagnostics of grain crop seeds intended for export
Зерновое хозяйство России, 2022 Russia occupies a leading position among the largest wheat producers and exporters. One of the key requirements for the biological safety of domestic grain products is the phytosanitary requirements of importing countries.
E. R. Ruchkov +3 more
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Development of a multiplex pcr method for direct detection of common mastitis pathogens in bovine milk samples [PDF]
Kafkas Universitesi Veteriner Fakültesi Dergisi, 2017 The aim of this study was to evaluate a simple and rapid DNA extraction method combined with a multiplex polymerase chain reaction (mPCR) for the identification of the major mastitis pathogens (Staphylococcus aureus, Streptococcus agalactiae, Escherichia
Recep KALIN +4 more
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Comparative analysis of reagent kits for DNA extraction from dry blood stains
Медицинская иммунология, 2023 Neonatal screening is a mandatory newborn screening procedure that detects the presence of genetic diseases. Dry blood stains are used for mass screening of children.
A. V. Sedykh +3 more
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Magnetofection and isolation of DNA using polyethyleneimine functionalized magnetic iron oxide nanoparticles [PDF]
Royal Society Open Science, 2018 This study was carried out to develop a simple and efficient method to isolate DNA directly from biological samples using iron oxide nanoparticles (IONPs) functionalized with polyethyleneimine (PEI).
Adheesha N. Danthanarayana +4 more
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Buletin Agroteknologi, 2020 The genetic studies of individual plants, especially self-pollinated species like chickpea need to be evaluated at the DNA level with the help of molecular markers for identifying genetic variations among the plants.
Umavathi Saraswathi +1 more
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