Results 11 to 20 of about 1,362,179 (211)

Preservation and maceration of amazon açai leaflet tissue to obtain genomic DNA

Bioscience Journal, 2019
The objective of this study was to test the efficiency of preservation and maceration methods for Euterpe precatoria leaflet tissue to obtain genomic DNA for molecular studies. The leaflets of E.
Hellen Sandra Freires da Silva Azêvedo, Polinar Bandeira Rufino, José Marlo Araújo de Azevedo, Luciélio Manoel da Silva, Lúcia Helena de Oliveira Wadt, Tatiana de Campos   +5 more
doaj   +1 more source

DNA isolation in processed chicken meat products (nugget) using modified DNeasy Mericon Food kit (Qiagen)

Ho Chi Minh City Open University Journal of Science - Engineering and Technology, 2022
DNA isolation is one of the important steps in conducting PCR-based or real-time PCR-based molecular analysis. This study aims to test the quality of DNA isolation extracted from samples of processed food products from chicken meat (chicken nuggets). The
Alfi Sophian, Utaminingsih Sri, Utami Dyah Sofia   +2 more
doaj   +1 more source

On the isolation of TI-plasmid from Agrobacterium tumefaciens [PDF]

, 1976
An efficient lysis method for Agrobacterium cells was developed, which allows a reproducible isolation of the tumor inducing (TI)-plasmid. The lysis method is based on the sensitivity of this bacterium to incubation with lysozyme, n-dodecylamine,EDTA ...
Dons, Johannes JM, Krol, AJM, Ledeboer, Adrianus M, Schell, Jeff, Schilperoort, Robert A, Spier, F, Van Larebeke, Nicolas, Zaenen, Ivo   +7 more
core   +2 more sources

Analysis of Purity and Concentration Escherichia coli DNA by Boiling Method Isolation with Addition of Proteinase-K and RNase

Indonesian Journal of Medical Laboratory Science and Technology, 2023
Escherichia coli is a leading cause of Urinary Tract Infections (UTIs) in Indonesia, with approximately 180,000 cases reported annually. The more cases of UTIs, the more PCR diagnosis is needed with an accurate, fast, simple, and economical DNA ...
Bunga Rossa Lesiani, Yogi Khoirul Abror, Fusvita Merdekawati, Ai Djuminar   +3 more
doaj   +1 more source

Detection of toxigenic Clostridium difficile in pig feces by PCR

Veterinární Medicína, 2009
: Clostridium difficile is considered an important uncontrolled cause of neonatal diarrhea. Also, the presence of bacteria in the feces of the animal could represent a zoonosic risk for the contamination of meat products.
S. Alvarez-Perez, P. Alba, J.L. Blanco, M.E. Garcia   +3 more
doaj   +1 more source

Comparison of DNA Isolation Methods that Derived from Leaves of a Potential Anti-Cancer Rodent Tuber (Typhonium flagelliforme) Plant

International Journal of Agriculture System, 2022
The content of polysaccharides, polyphenols, proteins, and RNA compounds is the main problem often found in Plants DNA isolation, which inhibit the process of DNA isolation.
Gemilang Rahmadara, Nurul Fitri Hanifah, Rismayanti Rismayanti, Devit Purwoko, Andi Rochandi, Teuku Tajuddin   +5 more
doaj   +1 more source

Comparative analysis of the DNA isolated from thyme leaves using different methods

Труды по прикладной ботанике, генетике и селекции, 2020
Background. The base for a molecular analysis is DNA of high quality. For DNA isolation, different kits or classical methods are used. For mass analysis, isolation with kits is a very expensive process.
I. V. Bulavin, O. A. Grebennikova, V. A. Brailko, S. A. Feskov, I. V. Mitrofanova   +4 more
doaj   +1 more source

Chromosome Centromeres: Structural and Analytical Investigations with High Resolution Scanning Electron Microscopy in Combination with Focused Ion Beam Milling [PDF]

, 2009
Whole mount mitotic metaphase chromosomes of different plants and animals were investigated with high resolution field emission scanning electron microscopy (FESEM) to study the ultrastructural organization of centromeres, including metacentric ...
Schroeder-Reiter, E., Wanner, Gerhard
core   +1 more source

Detection of PCR inhibition in food and feed with a synthetic plasmid

Czech Journal of Food Sciences, 2017
We present a successful use of the plasmid inhibition detection and DNA isolation protocol optimisation for four food/feed samples in qPCR analysis of the sequence coding for chloroplast tRNA-Leu: two meat meal samples and two samples made of cranberries
Tereza Sovová, Barbora Křížová, Lenka Drábková, Jaroslava Ovesná   +3 more
doaj   +1 more source

Sex determination from the pulp tissue of deciduous teeth exposed to natural soil and wet clay - A PCR study

Indian Journal of Dental Research, 2020
Context: Dental tissue remains are the toughest, and chemically, the most stable tissue in the body. Its high resilience in the events of fire and bacterial decomposition makes them vital for DNA analysis by PCR method.
Prachi Suman, R Manju, Veena A Shetty, Amitha M Hegde, Muthtamil, Shama Rao   +5 more
doaj   +1 more source