DNA methylation dynamics associated with long-term isolation of simulated space travel
iScience, 2022 Long-term isolation is one of the risk factors that astronauts will encounter in spaceflight. At present, few researches have explored DNA methylation dynamics during long-term isolation.
Fei Hou +8 more
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International Journal of Tropical Veterinary and Biomedical Research, 2018 Elephant Training Center (ETC) Way Kambas National Park (WKNP) was built to support human-elephant mitigation conflict. The small population of captive sumatran elephant in ETC WKNP need a comprehensive strategy in order to maintain the genetic variation
Elly L. Rustiati +9 more
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Optimization of DNA extraction from human urinary samples for mycobiome community profiling. [PDF]
, 2019 IntroductionRecent data suggest the urinary tract hosts a microbial community of varying composition, even in the absence of infection. Culture-independent methodologies, such as next-generation sequencing of conserved ribosomal DNA sequences, provide an
Ackerman, A Lenore +8 more
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Restriction Fragment Length Polymorphism Linkage Map for Arabidopsis thaliana [PDF]
, 1988 We have constructed a restriction fragment length polymorphism linkage map for the nuclear genome of the flowering plant Arabidopsis thaliana. The map, containing 90 randomly distributed molecular markers, is physically very dense; >50% of the genome is ...
Bowman, John L. +4 more
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Isolation by distance in a population of a small land snail Trochoidea geyeri : evidence from direct and indirect methods [PDF]
, 2006 Population structure was estimated in a continuous population of a small land snail (Trochoidea geyeri). Mark-recapture experiments and randomly amplified polymorphic DNA analyses indicate that the population structure can be described by the isolation ...
Bahl, Andreas +2 more
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Comparative analysis of human DNA extraction methods and mitochondrial DNA HV1 and HV2 haplogroup determination [PDF]
Kragujevac Journal of Science, 2020 The high integrity of extracted DNA is necessary for the determination of human haplogroups, based on the mitochondrial DNA (mtDNA) marker sequences. The study aims were to isolate the total DNA from selected human samples and establish a protocol that ...
Nikezić Aleksandra G. +8 more
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The Miraprep: A Protocol that Uses a Miniprep Kit and Provides Maxiprep Yields. [PDF]
PLoS ONE, 2016 Plasmid purification is a basic tool of molecular biologists. Although the development of plasmid isolation kits utilizing silica spin columns reduced the time and labor spent on plasmid purification, achieving large plasmid DNA yields still requires ...
Mira I Pronobis +2 more
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Improved Screening of cDNAs Generated by mRNA Differential Display Enables the Selection of True Positives and the Isolation of Weakly Expressed Messages [PDF]
, 1997 The high percentage of false positives generated by differential display (as high as 85%) has previously limited the potential of the method. This report describes an efficient methodology that enables false positives to be discarded prior to cloning ...
Bouquin, Thomas +6 more
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High-quality DNA extraction for Desmodium gangeticum: A medicinal shrub of Shivalik Himalayas
Journal of Medicinal Plants for Economic DevelopmentBackground: Desmodium gangeticum, a crucial medicinal shrub from the Himalayan Shivalik region, is an economically important species globally. It contains a vast range of secondary metabolites, majorly polyphenols, that interfere with high-quality ...
Shiwani Singh +2 more
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A novel cost effective and high-throughput isolation and identification method for marine microalgae [PDF]
, 2014 Background: Marine microalgae are of major ecologic and emerging economic importance. Biotechnological screening schemes of microalgae for specific traits and laboratory experiments to advance our knowledge on algal biology and evolution strongly benefit
Jahn, Martin T +2 more
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