Results 31 to 40 of about 1,362,179 (211)

Comparison of methods for isolating fungal DNA

open access: yesPractical Laboratory Medicine, 2021
Objectives: The main aim of this work was to compare the methods of DNA isolation in the moulds of genus Mucorales with special regard to the amount and purity of the DNA acquired. The acquired DNA was then amplified by specific real-time PCR.
Ilaria Galliano   +7 more
doaj   +1 more source

A binary vector for transferring genomic libraries to plants [PDF]

open access: yes, 1986
The transformation of mutant plants with a complete recombinant library derived from wild-type DNA followed by assay of transformed plants for complementation of the mutant phenotype is a promising method for the isolation of plant genes.
Simoens, Chris   +8 more
core   +1 more source

Comparison of gender determination from dental pulp and dentin after exposure to various environmental conditions: A polymerase chain reaction-based SRY gene study

open access: yesContemporary Clinical Dentistry, 2019
Purpose: Sex determination becomes the first priority by a forensic investigator in the process of identification of bodies mutilated beyond recognition due to mass disasters.
Ruheena Khan   +2 more
doaj   +1 more source

A fully automatable enzymatic method for DNA extraction from plant tissues [PDF]

open access: yes, 2005
Background DNA extraction from plant tissues, unlike DNA isolation from mammalian tissues, remains difficult due to the presence of a rigid cell wall around the plant cells.
Jean-François Manen   +4 more
core   +2 more sources

Comparison of methods for isolating fungal DNA

open access: yesCzech Journal of Food Sciences, 2011
In this study methods of fungal DNA isolation were optimised and compared. The aim of the isolation processes was to obtain DNA of sufficient quality and quantity necessary for its amplification, as most detection techniques require DNA amplification ...
Petra MOŤKOVÁ, Jarmila VYTŘASOVÁ
doaj   +1 more source

An efficient protocol to perform genetic traceability of tissue and foods from Geoffroea decorticans

open access: yes, 2019
The quality of a DNA isolation method depends, among others, on the target tissue and the metabolites therein. Geoffroea decorticans Burkart (chanar) is a species that has nutritional and pharmacological potential.
Aguayo, F.   +3 more
core   +1 more source

Identification of functional cis-regulatory elements by sequential enrichment from a randomized synthetic DNA library [PDF]

open access: yes, 2013
BACKGROUND: The identification of endogenous cis-regulatory DNA elements (CREs) responsive to endogenous and environmental cues is important for studying gene regulation and for biotechnological applications but is labor and time intensive. Alternatively,
Ahmadinejad, N.   +3 more
core   +2 more sources

Comparison of DNA Isolation Results with Simple Methods and Kits in Samples of Psidium guajava Leaves

open access: yesBiotropic: The Journal of Tropical Biology, 2018
DNA isolation is one of a series of methods that must be carried out on the basic techniques of Molecular Biology Analysis. Especially PCR-based molecular marking techniques. Many ways are done in DNA isolation. This study discusses the comparison of the
Yuanita Rachmawati   +1 more
doaj   +1 more source

Efficient DNA isolation from moroccan arar tree [Tetraclinis articulata (Vahl) Masters] leaves and optimization of the rapd-pcr molecular technique [PDF]

open access: yes, 2010
Efficient DNA isolation from Moroccan Arar tree [Tetraclinis articulata (Vahl) Masters] leaves and optimization of the RAPD-PCR molecular technique. Molecular genetic analysis of Arar tree [Tetraclinis articulata (Vahl) Masters] is often limited by the
Abourouh, Mohamed   +4 more
core   +2 more sources

PCR reaction testing by using microsatellite DNA markers on Bali Myna samples (Leucopsar rothschildi Stressemann, 1912)

open access: yesJurnal Biologi Udayana
Genetic research requires a good quality and quantity of DNA template and appropriate conditions to obtain optimal PCR results. This study aimed to evaluate and compare PCR reaction conditions and compositions in Bali Myna (Leucopsar rothschildi ...
Utami Lestari Budiawati   +2 more
doaj   +1 more source

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