Results 121 to 130 of about 2,120,704 (297)
Elucidating the genetic mechanisms governing cytosine base editing outcomes through CRISPRi screens
Nature CommunicationsCytosine base editors enable programmable and efficient genome editing using an intermediate featuring a U•G mismatch across from a DNA nick. This intermediate facilitates two major outcomes, C•G to T•A and C•G to G•C point mutations, and it is not ...
Sifeng Gu +5 more
doaj +1 more source
Advanced Science, EarlyView.FES‐derived MGE spheroids exhibit progenitor‐stage alterations in developmental trajectory and hypoxia‐responsive transcriptional programs, followed by functional disruption. Gestational hypoxia recapitulates impaired progenitor proliferation, shortened cell‐cycle progression, interneuron developmental abnormalities, and schizophrenia‐like behaviors in
Peiyan Ni +17 more
wiley +1 more source
DNA Loop Repair by Escherichia Coli Cell Extracts
, 2009 The nick-directed DNA repair of a set of M13mp18 derived heteroduplexes containing 8-, 12-, 16-, 22-, 27-, 45-, and 429-nucleotide loops was determined by in vitro assay.
FANG, WOEI-HORNG;WANG, BO-JENG;WANG, CHIANG-HUI;LI, SHU-CHEN;CHANG, YU-TING;CHUANG, YI-KUANG;LEE, CHUN-NAN +1 more
core
Advanced Science, EarlyView.An α‐helical peptide, TAB12, designed to mimic the TRIM28 binding interface, competitively disrupts the TRIM28‐BRD7 interaction, thereby blocking ubiquitin‐mediated degradation of the tumor suppressor BRD7. This stabilization unleashes potent anti‐tumor effects across multiple tumor types with a favorable safety profile, offering a feasible strategy ...
Qingqing Wei +10 more
wiley +1 more source
Advanced Science, EarlyView.A rare de novo IFT122‐A773E variant is identified in idiopathic pediatric uveitis and shown to exacerbate retinal inflammation and barrier dysfunction. Mechanistically, the variant enhances IFT43 interaction, elevates calcium signaling, and activates the MEK/ERK/FRA1 axis, revealing a previously unrecognized cilia‐associated pathway that may increase ...
Qian Zhou +18 more
wiley +1 more source
DNA mismatch repair deficient (dMMR) endometrial cancer
Taiwanese Journal of Obstetrics & Gynecology, 2022 Chia-Hao Liu +2 more
doaj +1 more source
Repair Patch Analysis of DNA Mismatch and Loop Repair
, 2007 演化過程中所保留下來的MMR可以藉由修復DNA複製錯誤，抑制發生在部分解離DNA序列之間的非同質性重組，與不同的DNA損害作用以及一些其他的功能來維持真核與原核生物基因體的穩定性。在核酸配對錯誤復上已有相當透澈的瞭解。但是大型核酸環的修復，一個迥異於配對錯誤的修復機制，卻是個未知的系統。因此研究這兩種修復系統間的差異，將是個令人感興趣的議題。 人類特異股配對修復及大型核酸環修復均需外加dNTP，同時修復反應也受到aphidicolin的抑制 ...
Chen, Ying-Kun, 陳盈坤
core
Dual‐line Genome‐scale CRISPR Screening Enables Robust Target Gene Discovery
Advanced Science, EarlyView.A species‐optimized CRISPR platform integrates efficient piggyBac delivery, genome‐scale sgRNA libraries, and parallel screening in two independently engineered Bactrocera dorsalis Cas9 cell lines. Cross‐line consensus analysis filters line‐specific effects, enriches candidates with reproducible in vivo phenotypes, and reveals conserved, species ...
Ziniu Li +9 more
wiley +1 more source
Advanced Science, EarlyView.This study developed a dual‐targeting biomimetic nanozyme hybrid system (Cu‐CeO2@ABs‐FTP) and integrated it with HAMA hydrogel to fabricate a microneedle patch (CAF@MN). The patch promotes fibroblast‐mediated wound repair, targets macrophages to induce M2 polarization, and targets myofibroblasts to exert anti‐fibrotic effects, thereby enabling rapid ...
Hongyi Zhang +14 more
wiley +1 more source