Results 151 to 160 of about 70,351 (245)

Sequential, chromosome‐specific glutamine synthetase double knockout with Cas‐CLOVER establishes enhanced CHO platforms for cell line development

Biotechnology Progress, EarlyView.
This study established the first creation of a commercially available double GS knockout (DKO) from a single KO in CHO‐K1 cells, called CleanCut GS CHO using Cas‐CLOVER. When combined with the Harbor‐IN transposase system, CleanCut GS CHO cells demonstrated a highly antibody productivity.
Cintia Gomez Limia, Valeriya Steffey, Hsueh Che Cheng, Daniel Machado, Thomas Hart, Mark Cameron McHargue, Corey Brizzee, Jack Crawford   +7 more
wiley   +1 more source

Molecular and immune determinants of response in locally advanced deficient DNA mismatch repair/microsatellite instability-high gastric or gastroesophageal junction adenocarcinoma treated with neoadjuvant chemoimmunotherapy. [PDF]

Cell Commun Signal
Yu P, Huang X, Chen X, Chen H, Wang S, Wang D, Yan J, Chen J, Wei Q, Wu Z, Fang S, Chen H, Wang H, Zhu C, Huang L, Du Y, Bao H, Cheng X.   +17 more
europepmc   +1 more source

Self‐Calibrating Ratiometric DNA Aptamer Probe for Quantitative ATP Imaging in Living Cells

Chemistry – A European Journal, EarlyView.
We developed the RFA–ECHO probe, an internally calibrated ATP sensor that remains responsive across millimolar ATP levels. Its self‐correcting Cy5/ECHO ratio ensures accurate quantification independent of probe uptake. Compared with FRET‐based sensors, it yields a robust ratiometric shift, distinguishing high‐ATP cancer cells from normal ones, enabling
Yunsong Xu, Kunihiko Morihiro, Rui Cong, Samruddhi Maheshwar Patil, Idoia Wille, Akimitsu Okamoto   +5 more
wiley   +1 more source

Real-World Impact of Metformin on Outcomes in Patients with Deficient DNA Mismatch Repair and Microsatellite Instability (dMMR/MSI) Colorectal Cancer Treated with Immune Checkpoint Inhibitors. [PDF]

Cancers (Basel)
Gupta G, Sadeghipour N, Bicer F, Elliott A, Hinton A, Lou E, Vanderwalde AM, Ozluk AA, Khushman MM, Malla M, Outlaw D, Hussaini SQ, El-Rayes BF, Akce M.   +13 more
europepmc   +1 more source

Mini‐Hairpin DNA: Extraordinarily Stable Structure and Its Applications

Chemistry – A European Journal, EarlyView.
Mini‐hairpin DNA fragments containing GCGAAGC and GCGAAAGC form exceptionally stable hairpin‐like structures, with only two G−C pairs and one sheared G−A pair, remaining undenatured in 7 M urea and resisting nuclease digestion. These mini‐hairpin sequences frequently appear in replication and transcription origins, influence DNA sequencing fidelity ...
Michiko Kimoto, Ichiro Hirao
wiley   +1 more source

Comparison of clinicopathological parameters with the presence of Epstein-Barr virus and the absence of DNA mismatch repair proteins in gastric adenocarcinomas. [PDF]

Asian Biomed (Res Rev News)
Eyeoğlu Ö, Kayaçetin S.
europepmc   +1 more source

Rational Design Strategies for Stimuli‐Responsive DNAzymes Using Modified and Artificial Nucleotides

Chemistry – A European Journal, EarlyView.
Activity control of RNA‐cleaving DNAzymes in response to external stimuli has attracted growing interest for applications in DNA‐based diagnostics, therapeutics, and dynamic DNA nanotechnology. This review summarizes rational design strategies for stimuli‐responsive DNAzymes using modified or artificial nucleotides, including split and caged DNAzymes ...
Yusuke Takezawa, Mitsuhiko Shionoya
wiley   +1 more source

Genomic Alterations in DNA Mismatch Repair Genes Across Different Cancer Types. [PDF]

JCO Precis Oncol
Holla VR, Kahle MP, Kim SH, Ronaghy A, Yang RK, Patel KP, Routbort MJ, Overman MJ, Dumbrava EE, Mills Shaw KR, Karp DD, Meric-Bernstam F.   +11 more
europepmc   +1 more source

In Vitro Live Cell Imaging Reveals Nuclear Dynamics and Role of the Cytoskeleton During Asymmetric Division of Pollen Mitosis I in Nicotiana Benthamiana

Cytoskeleton, EarlyView.
ABSTRACT Pollen is a male gametophyte of angiosperms. Following meiosis, the microspore undergoes an asymmetric division called pollen mitosis I (PMI), which produces two cells of different sizes: a large vegetative cell and a small generative cell. Polarized nuclear migration and positioning during PMI are important for successful pollen development ...
Yoko Mizuta, Masako Igarashi, Tomomi Shinagawa, Ikuma Kaneshiro, Haoto Anan, Daisuke Kurihara   +5 more
wiley   +1 more source

DNA Mismatch Repair Deficiency as a Biomarker in Sarcoma. [PDF]

Surg Oncol Insight
Denu RA, Quintana-Perez CD, Wangsiricharoen S, Ingram DR, Wani KM, Lazar AJ, Ratan R, Roland CL, You YN.   +8 more
europepmc   +1 more source