Results 31 to 40 of about 405,469 (354)

Structure of Type IIL Restriction-Modification Enzyme MmeI in Complex with DNA Has Implications for Engineering New Specificities. [PDF]

open access: yesPLoS Biology, 2016
The creation of restriction enzymes with programmable DNA-binding and -cleavage specificities has long been a goal of modern biology. The recently discovered Type IIL MmeI family of restriction-and-modification (RM) enzymes that possess a shared target ...
Scott J Callahan   +6 more
doaj   +1 more source

A new and versatile method for the successful conversion of AFLP-TM markers into simple single locus markers [PDF]

open access: yes, 2003
Genetic markers can efficiently be obtained by using amplified fragment length polymorphism (AFLP) fingerprinting because no prior information on DNA sequence is required.
Brugmans, B.W.   +4 more
core   +2 more sources

Suppression of APOBEC3-mediated restriction of HIV-1 by Vif

open access: yesFrontiers in Microbiology, 2014
The APOBEC3 restriction factors are a family of deoxycytidine deaminases that are able to suppress replication of viruses with a single-stranded DNA intermediate by inducing mutagenesis and functional inactivation of the virus.
Yuqing eFeng   +3 more
doaj   +1 more source

The Hyperthermophilic Restriction-Modification Systems of Thermococcus kodakarensis Protect Genome Integrity

open access: yesFrontiers in Microbiology, 2021
Thermococcus kodakarensis (T. kodakarensis), a hyperthermophilic, genetically accessible model archaeon, encodes two putative restriction modification (R-M) defense systems, TkoI and TkoII.
Kelly M. Zatopek   +6 more
doaj   +1 more source

2b-RAD genotyping for population genomic studies of Chagas disease vectors: Rhodnius ecuadoriensis in Ecuador [PDF]

open access: yes, 2017
Background: Rhodnius ecuadoriensis is the main triatomine vector of Chagas disease, American trypanosomiasis, in Southern Ecuador and Northern Peru.
Andersson, Björn   +10 more
core   +4 more sources

Structural and functional analysis of the symmetrical Type I restriction endonuclease R.EcoR124I<sub>NT</sub>

open access: yesPLoS ONE, 2012
Type I restriction-modification (RM) systems are comprised of two multi-subunit enzymes, the methyltransferase (∼160 kDa), responsible for methylation of DNA, and the restriction endonuclease (∼400 kDa), responsible for DNA cleavage. Both enzymes share a
James E Taylor   +4 more
doaj   +1 more source

Restriction enzyme-mediated DNA integration in Coprinus cinereus

open access: yesMolecular and General Genetics MGG, 1997
Restriction enzyme-mediated DNA integration (REMI) has recently received attention as a new technique for the generation of mutants by transformation in fungi. Here we analyse this method in the basidiomycete Coprinus cinereus using the homologous pabI gene as a selectable marker and the restriction enzymes BamHI, EcoRI and PstI.
Granado, J.D.   +3 more
openaire   +3 more sources

Sequence organization of feline leukemis virus DNA in infected cells [PDF]

open access: yes, 1980
A restriction site map has been deduced of unintegrated and integrated FeLV viral DNA found in human RD cells after experimental infection with the Gardner-Arnstein strain of FeLV.
Casey, James W.   +3 more
core   +1 more source

Using of Data Base to Determine the Restriction Sites and Drawing Restriction Map for Lipase Gene from Bacillus stearothermophilus

open access: yesمجلة مركز بحوث التقنيات الاحيائية, 2011
pecific data base was used for restriction enzymes (rebase) and related proteins, to design executive program in quick basic language to determine the restriction sites and drawing restriction map for the complete sequence of lipase gene from Bacillus ...
Hameed M. Jasim
doaj   +1 more source

Microarray-Based Analysis of Methylation Status of CpGs in Placental DNA and Maternal Blood DNA--Potential New Epigenetic Biomarkers for Cell Free Fetal DNA-Based Diagnosis. [PDF]

open access: yesPLoS ONE, 2015
Epigenetic markers for cell free fetal DNA in the maternal blood circulation are highly interesting in the field of non-invasive prenatal testing since such markers will offer a possibility to quantify the amount of fetal DNA derived from different ...
Lotte Hatt   +7 more
doaj   +1 more source

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