Results 31 to 40 of about 390,387 (330)
Type I restriction endonucleases are true catalytic enzymes [PDF]
, 2009 Type I restriction endonucleases are intriguing, multifunctional complexes that restrict DNA randomly, at sites distant from the target sequence. Restriction at distant sites is facilitated by ATP hydrolysis-dependent, translocation of double-stranded ...
Berge +42 more
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Restriction enzyme mapping of vaccinia virus DNA [PDF]
Journal of Virology, 1982 The cleavage sites for the restriction enzymes Bg/I, HindIII, KpnI, SalI, SmaI, and XhoI were located, from primary data, on the DNA isolated from the WR strain of vaccinia virus. Bg/I and SmaI divide the DNA into five segments which can be isolated by sucrose gradient centrifugation.
openaire +2 more sources
Suppression of APOBEC3-mediated restriction of HIV-1 by Vif
Frontiers in Microbiology, 2014 The APOBEC3 restriction factors are a family of deoxycytidine deaminases that are able to suppress replication of viruses with a single-stranded DNA intermediate by inducing mutagenesis and functional inactivation of the virus.
Yuqing eFeng +3 more
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Frontiers in Microbiology, 2021 Thermococcus kodakarensis (T. kodakarensis), a hyperthermophilic, genetically accessible model archaeon, encodes two putative restriction modification (R-M) defense systems, TkoI and TkoII.
Kelly M. Zatopek +6 more
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Highlights of the DNA cutters: a short history of the restriction enzymes [PDF]
Nucleic Acids Research, 2013 AbstractIn the early 1950’s, ‘host-controlled variation in bacterial viruses’ was reported as a non-hereditary phenomenon: one cycle of viral growth on certain bacterial hosts affected the ability of progeny virus to grow on other hosts by either restricting or enlarging their host range.
Loenen, Wil A M +4 more
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PLoS ONE, 2012 Type I restriction-modification (RM) systems are comprised of two multi-subunit enzymes, the methyltransferase (∼160 kDa), responsible for methylation of DNA, and the restriction endonuclease (∼400 kDa), responsible for DNA cleavage. Both enzymes share a
James E Taylor +4 more
doaj +1 more source
مجلة مركز بحوث التقنيات الاحيائية, 2011 pecific data base was used for restriction enzymes (rebase) and related proteins, to design executive program in quick basic language to determine the restriction sites and drawing restriction map for the complete sequence of lipase gene from Bacillus ...
Hameed M. Jasim
doaj +1 more source
Microarray-Based Analysis of Methylation Status of CpGs in Placental DNA and Maternal Blood DNA--Potential New Epigenetic Biomarkers for Cell Free Fetal DNA-Based Diagnosis. [PDF]
PLoS ONE, 2015 Epigenetic markers for cell free fetal DNA in the maternal blood circulation are highly interesting in the field of non-invasive prenatal testing since such markers will offer a possibility to quantify the amount of fetal DNA derived from different ...
Lotte Hatt +7 more
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An Efficient PCR-RFLP Method for the Rapid Identification of Korean Pyropia Species
Molecules, 2017 The present study utilizes polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis using partial plastid rbcL and mitochondrial trnC–trnP gene sequences to distinguish the six representative Pyropia species produced via ...
Yonguk Kim, Sung-Je Choi, Chulyung Choi
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EcoRV catalysis with a pre-bent substrate
AIP Advances, 2015 Enzymes are deformable molecules which often adapt their conformation to the substrate’s geometry. In the case of restriction enzymes acting on DNA, the substrate (DNA) is deformable also.
Daniel S. Sanchez, Giovanni Zocchi
doaj +1 more source