Results 31 to 40 of about 673,449 (404)
BioTechniques, 1999 Microsatellite loci are highly informative genetic markers useful for population genetic studies, linkage mapping and parentage determination. Methods to identify novel microsatellite loci commonly use subtractive hybridization to enrich smallinsert ...
Matthew B. Hamilton +3 more
doaj +1 more source
Mechanism of DNA Recognition by the Restriction Enzyme EcoRV
Journal of Molecular Biology, 2010 EcoRV, a restriction enzyme in Escherichia coli, destroys invading foreign DNA by cleaving it at the center step of a GATATC sequence. In the EcoRV-cognate DNA crystallographic complex, a sharp kink of 50 degrees has been found at the center base-pair step (TA).
Zahran M +3 more
openaire +5 more sources
Biomolecular computers with multiple restriction enzymes
Genetics and Molecular Biology, 2017 The development of conventional, silicon-based computers has several limitations, including some related to the Heisenberg uncertainty principle and the von Neumann “bottleneck”.
Sebastian Sakowski +5 more
doaj +1 more source
Alleviation of restriction by DNA condensation and non-specific DNA binding ligands [PDF]
, 2004 During conditions of cell stress, the type I restriction and modification enzymes of bacteria show reduced, but not zero, levels of restriction of unmethylated foreign DNA.
Dryden, D T F, Keatch, S A, Su, T J
core +2 more sources
Accelerated search kinetics mediated by redox reactions of DNA repair enzymes [PDF]
Biophysical Journal, volume 96, pp 3949-3958, (2009), 2009 A Charge Transport (CT) mechanism has been proposed in several papers (e.g., Yavin, et al. PNAS, v102 3546 (2005)) to explain the localization of Base Excision Repair (BER) enzymes to lesions on DNA. The CT mechanism relies on redox reactions of iron-sulfur cofactors that modify the enzyme's binding affinity.
arxiv +1 more source
Open Biology, 2015 Characterization of dynamic DNA methylomes in diverse phylogenetic groups has attracted growing interest for a better understanding of the evolution of DNA methylation as well as its function and biological significance in eukaryotes.
Shi Wang +10 more
semanticscholar +1 more source
BioTechniques, 2001 A method for generating limited representations of total bacterial DNA, without prior knowledge of the DNA sequence, has been developed. This method consists of three steps: digestion with two restriction enzymes, ligation of two oligonucleotide adapters
Aleksander Masny, Andrzej Plucienniczak
doaj +1 more source
Enhancement of PCRs by Partial Restriction Digestion of Genomic Templates
BioTechniques, 1999 Genomic DNA preparations derived from mammalian cells can often exhibit poor template activity in PCR, particularly when carried out on target sequences present at low copy number. Using genomic DNA bearing SV40 sequences integrated into host chromosomal
Raymond Vázquez, Mark L. Steinberg
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The Computational Power of Benenson Automata [PDF]
Theoretical Computer Science 344(2-3): 279-297, 2005, 2004 The development of autonomous molecular computers capable of making independent decisions in vivo regarding local drug administration may revolutionize medical science. Recently Benenson at el (2004) have envisioned one form such a ``smart drug'' may take by implementing an in vitro scheme, in which a long DNA state molecule is cut repeatedly by a ...
arxiv +1 more source
Restriction Fragment Length Polymorphism Linkage Map for Arabidopsis thaliana [PDF]
, 1988 We have constructed a restriction fragment length polymorphism linkage map for the nuclear genome of the flowering plant Arabidopsis thaliana. The map, containing 90 randomly distributed molecular markers, is physically very dense; >50% of the genome is ...
Bowman, John L. +4 more
core +1 more source