Results 31 to 40 of about 634,910 (347)
Structure of Type IIL Restriction-Modification Enzyme MmeI in Complex with DNA Has Implications for Engineering New Specificities. [PDF]
PLoS Biology, 2016 The creation of restriction enzymes with programmable DNA-binding and -cleavage specificities has long been a goal of modern biology. The recently discovered Type IIL MmeI family of restriction-and-modification (RM) enzymes that possess a shared target ...
Scott J Callahan +6 more
doaj +1 more source
Sequence organization of feline leukemis virus DNA in infected cells [PDF]
, 1980 A restriction site map has been deduced of unintegrated and integrated FeLV viral DNA found in human RD cells after experimental infection with the Gardner-Arnstein strain of FeLV.
Casey, James W. +3 more
core +1 more source
Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes
PLoS ONE, 2009 We have developed a protocol to assemble in one step and one tube at least nine separate DNA fragments together into an acceptor vector, with 90% of recombinant clones obtained containing the desired construct.
Carola Engler +3 more
semanticscholar +1 more source
Suppression of APOBEC3-mediated restriction of HIV-1 by Vif
Frontiers in Microbiology, 2014 The APOBEC3 restriction factors are a family of deoxycytidine deaminases that are able to suppress replication of viruses with a single-stranded DNA intermediate by inducing mutagenesis and functional inactivation of the virus.
Yuqing eFeng +3 more
doaj +1 more source
Frontiers in Microbiology, 2021 Thermococcus kodakarensis (T. kodakarensis), a hyperthermophilic, genetically accessible model archaeon, encodes two putative restriction modification (R-M) defense systems, TkoI and TkoII.
Kelly M. Zatopek +6 more
doaj +1 more source
PLoS ONE, 2012 Type I restriction-modification (RM) systems are comprised of two multi-subunit enzymes, the methyltransferase (∼160 kDa), responsible for methylation of DNA, and the restriction endonuclease (∼400 kDa), responsible for DNA cleavage. Both enzymes share a
James E Taylor +4 more
doaj +1 more source
Archaeal diversity in deep-sea sediments estimated by means of different Terminal-Restriction Fragment Length Polymorphisms (T-RFLP) protocols [PDF]
, 2009 Despite the increasing recognition of the quantitative importance of Archaea in all marine systems, the protocols for a rapid estimate of Archaeal diversity patterns in deep-sea sediments have been only poorly tested yet.
Danovaro, Roberto +3 more
core +1 more source
7-Deazaguanine modifications protect phage DNA from host restriction systems
Nature Communications, 2019 Genome modifications are central components of the continuous arms race between viruses and their hosts. The archaeosine base (G+), which was thought to be found only in archaeal tRNAs, was recently detected in genomic DNA of Enterobacteria phage 9g and ...
Geoffrey Hutinet +18 more
semanticscholar +1 more source
Restriction Enzyme Analysis of Tomato Chloroplast and Chromoplast DNA [PDF]
Plant Physiology, 1986 Plastid DNA was isolated from the chloroplasts of tomato (Lycopersicon esculentum var Traveler 76) leaves and the chromoplasts of ripe tomato fruit. Comparisons of the two DNAs were made by restriction endonuclease analysis using PvuII, HpaI, and Bg1I.
C M, Hunt, R C, Hardison, C D, Boyer
openaire +2 more sources
مجلة مركز بحوث التقنيات الاحيائية, 2011 pecific data base was used for restriction enzymes (rebase) and related proteins, to design executive program in quick basic language to determine the restriction sites and drawing restriction map for the complete sequence of lipase gene from Bacillus ...
Hameed M. Jasim
doaj +1 more source