Restriction enzyme digestion of heterochromatin in Drosophila nasuta [PDF]
, 1991 In situ digestion of metaphase and polytene chromosomes and of interphase nuclei in different cell types of Drosophila nasuta with restriction enzymes revealed that enzymes like AluI, EcoRI, HaeIII, Sau3a and SinI did not affect Giemsa-stainability of ...
Lakhotia, S. C., Tiwari, P. K.
core +1 more source
PCR-RFLP of mitochondrial DNA of swamp buffaloes in breeding station
Italian Journal of Animal Science, 2010 Methods have been devised for detecting polymorphisms in the D-loop regions using the PCR followed by restriction enzyme digestion to reveal restriction fragment length polymorphism.
M. Kamonpatana +6 more
doaj +1 more source
MetClo: methylase-assisted hierarchical DNA assembly using a single type IIS restriction enzyme
Nucleic Acids Research, 2018 Efficient DNA assembly is of great value in biological research and biotechnology. Type IIS restriction enzyme-based assembly systems allow assembly of multiple DNA fragments in a one-pot reaction.
Da Lin, C. O'Callaghan
semanticscholar +1 more source
A comparison of DNA cleavage by the restriction enzymes SalPI and PstI [PDF]
Nucleic Acids Research, 1980 Methods for obtaining highly active, exonuclease-free, stable preparations of the Streptomyces albus P restriction enzyme SalPI are described. SalPI and its isoschizomer PstI (from the taxonomically distant Providencia stuartii 164) both cleave their recognition sequence (5'-CTGCAG-3') to generate fragments terminating in tetranucleotide 3' extensions ...
Jacqueline A. Carter +3 more
openaire +3 more sources
Whole genome phylogeny for 21 Drosophila species using predicted 2b-RAD fragments [PDF]
PeerJ, 2013 Type IIB restriction endonucleases are site-specific endonucleases that cut both strands of double-stranded DNA upstream and downstream of their recognition sequences.
Arun S. Seetharam, Gary W. Stuart
doaj +2 more sources
Baboon endogenous virus genome. I. Restriction enzyme map of the unintegrated DNA genome of a primate retrovirus [PDF]
, 1980 A detailed restriction map was deduced for the genome of an endogenous retrovirus of a higher primate, that of baboon. The cleavage sites for 12 restriction enzymes were mapped. The unintegrated linear viral DNA intermediate that is produced by infection
Cohen, Maurice +6 more
core
Common patterns in type II restriction enzyme binding sites [PDF]
, 2005 Restriction enzymes are among the best studied examples of DNA binding proteins. In order to find general patterns in DNA recognition sites, which may reflect important properties of protein–DNA interaction, we analyse the binding sites of all known type
Beyer, Andreas +4 more
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FoxO1 signaling in B cell malignancies and its therapeutic targeting
FEBS Letters, EarlyView.FoxO1 has context‐specific tumor suppressor or oncogenic character in myeloid and B cell malignancies. This includes tumor‐promoting properties such as stemness maintenance and DNA damage tolerance in acute leukemias, or regulation of cell proliferation and survival, or migration in mature B cell malignancies.
Krystof Hlavac +3 more
wiley +1 more source
Eliminating unwanted patterns with minimal interference [PDF]
arXiv, 2021 Artificial synthesis of DNA molecules is an essential part of the study of biological mechanisms. The design of a synthetic DNA molecule usually involves many objectives. One of the important objectives is to eliminate short sequence patterns that correspond to binding sites of restriction enzymes or transcription factors.
arxiv
Structural Factors that Influence the Inhibition of Type II Restriction Enzymes by Minor Groove Binders [PDF]
, 2009 The objective of this thesis was to study whether heterocyclic dicationic compounds that are minor groove binders have the ability to inhibit the digestive properties of type II restriction enzymes which bind to the major groove of the DNA.
Nguyen, Ha Hoang
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