Results 61 to 70 of about 397,727 (310)

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

Sequence-specific double-strand cleavage of DNA by penta-N-methylpyrrolecarboxamide-EDTA·Fe(II) [PDF]

, 1983
In the presence of O2 and 5 mM dithiothreitol, penta-N-methylpyrrolecarboxamide-EDTA·Fe(II) [P5E·Fe(II)] at 0.5 µ M cleaves pBR322 plasmid DNA (50 µ M in base pairs) on opposite strands to afford discrete DNA fragments as analyzed by agarose gel ...
Dervan, Peter B., Schultz, Peter G.
core  

DNA Cloning without Restriction Enzyme and Ligase

BioTechniques, 1999
One common problem in using the traditional DNA cloning procedure is that suitable natural restriction sites are often unavailable for a given task. Creating new restriction sites is often time consuming. Here, I describe a simple technique of producing "customized cohesive ends" by a combination of PCR primer design and lambda exonuclease digestion ...
openaire   +3 more sources

Inhibiting stearoyl‐CoA desaturase suppresses bone metastatic prostate cancer by modulating cellular stress, mTOR signaling, and DNA damage response

FEBS Letters, EarlyView.
Bone metastasis in prostate cancer (PCa) patients is a clinical hurdle due to the poor understanding of the supportive bone microenvironment. Here, we identify stearoyl‐CoA desaturase (SCD) as a tumor‐promoting enzyme and potential therapeutic target in bone metastatic PCa.
Alexis Wilson, Mackenzie K Herroon, Shane Mecca, Laimar C Garmo, Jacob Lindquist, Shrila Rajendran, Steve M. Patrick, Izabela Podgorski   +7 more
wiley   +1 more source

Meat molecular detection: sensitivity of polymerase chain reaction-restriction fragment length polymorphism in species differentiation of meat from animal origin [PDF]

, 2007
Three restriction enzymes were used in Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) using the mitochondrial cytochrome b region to establish a differential diagnosis which detect and discriminate between three meat ...
Chai, Lay Ching, Cheah, Yoke Kqueen, Gunsalam, Jurin Wolmon, Mat Issa @ Zakaria, Zuraini, Mohamad Ghazali, Farinazleen, Ong, Sang Bing, Radu, Son, Robin, Tunung, Yuli, Haryani   +8 more
core  

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Comparative analysis of 18S rRNA genes from Myxobolus aeglefini Auerbach, 1906 isolated from cod (Gadus morhua), Plaice (Pleuronectes platessa) and dab (Limanda limanda), using PCR-RFLP [PDF]

, 2002
The myxosporean parasite Myxobolus aeglefini is a marine species, which can be found in the cartilage of mainly gadid fish species. The parasite has, however, been recorded in the flatfish plaice (Pleuronectes platessa) and dab (Limanda limanda).
Buchmann, K., Koie, M., Nielsen, C. V., Székely, Csaba   +3 more
core  

Architecturally diverse proteins converge on an analogous mechanism to inactivate Uracil-DNA glycosylase [PDF]

, 2013
Uracil-DNA glycosylase (UDG) compromises the replication strategies of diverse viruses from unrelated lineages. Virally encoded proteins therefore exist to limit, inhibit or target UDG activity for proteolysis.
A. R. Cole, Acharya, Barrett, Berkner, Broennimann, dos Reis, Duncan, Emsley, Freitag, G. Baltulionis, Haushalter, Hennessy, Huang, Jern, K. Ryzhenkova, Kabsch, Katz, Kiljunen, Kleywegt, Koulis, Martomo, McCoy, Newman, P. Hornyak, Pearl, Perez-Lago, Putnam, Putnam, R. Savva, S. Ofer, Savva, Savva, Savva, Schorman, Sharp, Takahashi, Tye, Warner, Warren, Winn   +39 more
core   +1 more source

Structural biology of ferritin nanocages

FEBS Letters, EarlyView.
Ferritin is a conserved iron‐storage protein that sequesters iron as a ferric mineral core within a nanocage, protecting cells from oxidative damage and maintaining iron homeostasis. This review discusses ferritin biology, structure, and function, and highlights recent cryo‐EM studies revealing mechanisms of ferritinophagy, cellular iron uptake, and ...
Eloise Mastrangelo, Flavio Di Pisa
wiley   +1 more source

Cytosine-to-Uracil Deamination by SssI DNA Methyltransferase [PDF]

, 2013
The prokaryotic DNA(cytosine-5)methyltransferase M.SssI shares the specificity of eukaryotic DNA methyltransferases (CG) and is an important model and experimental tool in the study of eukaryotic DNA methylation.
A Beletskii, A Jeltsch, AN Sharath, Antal Kiss, AS Yang, B Bandaru, B Bandaru, B Chaikind, BM Hall, BTF van der Gun, DM Woodcock, EA Raleigh, EU Selker, EV Koudan, G Pósfai, GL Xu, H Gowher, I Stier, Ildikó Stier, J Sambrook, JC Shen, JC Shen, JC Shen, JM Zingg, JM Zingg, KM Reinisch, LA Frederico, LM Guzman, M Frommer, M Wyszynski, Martin G. Marinus, MH Sibley, P Rathert, P Renbaum, PA Waite-Rees, PM Warnecke, R Métivier, R Shapiro, RJ Roberts, RY-H Wang, S Kumar, SE Luria, T Durfee, TA Kunkel, UT Sankpal, WA Rosche, X Cheng, X Zhang   +47 more
core   +3 more sources