Results 11 to 20 of about 79,794 (267)
The Effect of Dimethyl Sulfoxide on Supercoiled DNA Relaxation Catalyzed by Type I Topoisomerases [PDF]
BioMed Research International, 2015 The effects of dimethyl sulfoxide (DMSO) on supercoiled plasmid DNA relaxation catalyzed by two typical type I topoisomerases were investigated in our studies.
Bei Lv +4 more
semanticscholar +4 more sources
Eukaryotic DNA topoisomerases: two forms of type I DNA topoisomerases from HeLa cell nuclei. [PDF]
Proceedings of the National Academy of Sciences, 1981 Two type I DNA topoisomerases have been purified to homogeneity from the nuclei of HeLa cells. One topoisomerase has a peptide molecular weight of 100,000 and the other, a molecular weight of 67,000. Several lines of evidence indicate that these two topoisomerases are closely related, (a) Both exhibit similar enzymatic activities on DNA.
Leroy F. Liu, Kathryn G. Miller
semanticscholar +3 more sources
SUMO: A Swiss Army Knife for Eukaryotic Topoisomerases
Frontiers in Molecular Biosciences, 2022 Topoisomerases play crucial roles in DNA metabolism that include replication, transcription, recombination, and chromatin structure by manipulating DNA structures arising in double-stranded DNA.
Yilun Sun, John L. Nitiss, Yves Pommier
doaj +1 more source
Antibiotics, 2023 Novel bacterial topoisomerase inhibitors (NBTIs) are a new class of antibacterial agents that target bacterial type II topoisomerases (DNA gyrase and topoisomerase IV). Our recently disclosed crystal structure of an NBTI ligand in complex with DNA gyrase
Maja Kokot +5 more
doaj +1 more source
Transcription facilitated genome-wide recruitment of topoisomerase I and DNA gyrase. [PDF]
PLoS Genetics, 2017 Movement of the transcription machinery along a template alters DNA topology resulting in the accumulation of supercoils in DNA. The positive supercoils generated ahead of transcribing RNA polymerase (RNAP) and the negative supercoils accumulating behind
Wareed Ahmed +5 more
doaj +1 more source
A Type I DNA Topoisomerase from Trypanosoma cruzi [PDF]
European Journal of Biochemistry, 1983 A type I DNA topoisomerase has been isolated from the nuclei of the flagellate Trypanosoma cruzi, using poly(ethylene glycol) fractionation and chromatography on hydroxyapatite and on phosphocellulose. The relaxation activity was ATP‐independent, enhanced by Mg2+ and spermidine. The enzyme removed supercoils from negative and positive superhelical DNAs.
G F, Riou +4 more
openaire +2 more sources
55.2, a phage T4 ORFan gene, encodes an inhibitor of Escherichia coli topoisomerase I and increases phage fitness. [PDF]
PLoS ONE, 2015 Topoisomerases are enzymes that alter the topological properties of DNA. Phage T4 encodes its own topoisomerase but it can also utilize host-encoded topoisomerases. Here we characterized 55.2, a phage T4 predicted ORF of unknown function.
Yves Mattenberger +2 more
doaj +1 more source
Inhibition of Zn(II) binding type IA topoisomerases by organomercury compounds and Hg(II). [PDF]
PLoS ONE, 2015 Type IA topoisomerase activities are essential for resolving DNA topological barriers via an enzyme-mediated transient single strand DNA break. Accumulation of topoisomerase DNA cleavage product can lead to cell death or genomic rearrangement.
Bokun Cheng +4 more
doaj +1 more source
Methanosarcina acetivorans C2A topoisomerase IIIα, an archaeal enzyme with promiscuity in divalent cation dependence. [PDF]
PLoS ONE, 2011 Topoisomerases play a fundamental role in genome stability, DNA replication and repair. As a result, topoisomerases have served as therapeutic targets of interest in Eukarya and Bacteria, two of the three domains of life.
Raymond Morales +3 more
doaj +1 more source
Flavonoids in Helichrysum pamphylicum Inhibit Mammalian Type I DNA Topoisomerase [PDF]
Zeitschrift für Naturforschung C, 2008 DNA topoisomerases are important targets for cancer chemotherapy. We investigated the effects of a methanolic extract of Helichrysum pamphylicum on mammalian DNA topoisomerase I via in vitro plasmid supercoil relaxation assays. The extracts manifested a considerable inhibition of the enzyme’s activity in a dose-dependent manner.
Topcu, Zeki +3 more
openaire +3 more sources