Results 171 to 180 of about 8,858 (210)

Insights into the Quality of DnaA Boxes and Their Cooperativity

Journal of Molecular Biology, 2006
Plasmids carrying the mioC promoter region with its two DnaA boxes are as efficient in titration of DnaA protein as plasmids carrying a replication-inactivated oriC region with its five DnaA boxes. The two DnaA boxes upstream of the mioC promoter were mutated in various ways to study the cooperativity between the DnaA boxes, and to study in vivo the in
Flemming G Hansen, Bjarke Bak Christensen, Tove Atlung   +2 more
exaly   +5 more sources

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Transcriptional repression of the dnaA gene of Escherichia coli by dnaA protein

Molecular Genetics and Genomics, 1987
The promoter region of the dnaA gene and of a gene which encodes a 16 kDa protein contain sites which are recognized and bound by dnaA protein. Using assays of run-off transcription of restriction fragments, purified dnaA protein specifically repressed transcription from both dnaA promoters and from the promoter for the 16 KD gene to almost ...
Jon M Kaguni
exaly   +3 more sources

DnaA protein binding to individual DnaA boxes in the Escherichia coli replication origin, oriC [PDF]

EMBO Journal, 1997
The formation of nucleoprotein complexes between the Escherichia coli initiator protein DnaA and the replication origin oriC was analysed in vitro by band-shift assays and electron microscopy. DnaA protein binds equally well to linear and supercoiled oriC substrates as revealed by analysis of the binding preference to individual DnaA boxes (9-mer ...
Rudi Lurz, Walter Messer
exaly   +3 more sources

Bacterial replication initiator DnaA. Rules for DnaA binding and rolesof DnaA in origin unwinding and helicase loading

Biochimie, 2001
We review the processes leading to the structural modifications required for the initiation of replication in Escherichia coli, the conversion of the initial complex to the open complex, loading of helicase, and the assembly of two replication forks.
W, Messer, F, Blaesing, D, Jakimowicz, M, Krause, J, Majka, J, Nardmann, S, Schaper, H, Seitz, C, Speck, C, Weigel, G, Wegrzyn, M, Welzeck, J, Zakrzewska-Czerwinska   +12 more
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Titration of DnaA protein by oriC DnaA-boxes increases dnaA gene expression in Escherichia coli.

The EMBO Journal, 1987
Binding of the DnaA protein to its binding sites, the DnaA-boxes (TTATCCACA), was measured by a simple physiological approach. The presence of extra DnaA-boxes in growing cells leads to a derepression of dnaA gene expression, measured as beta-galactosidase activity of a dnaA-lacZ fusion polypeptide.
F G, Hansen, S, Koefoed, L, Sørensen, T, Atlung   +3 more
openaire   +2 more sources

Functional domains of DnaA proteins

Biochimie, 1999
Functional domains of the initiator protein DnaA of Escherichia coli have been defined. Domain 1, amino acids 1-86, is involved in oligomerization and in interaction with DnaB. Domain 2, aa 87-134, constitutes a flexible loop. Domain 3, aa 135-373, contains the binding site for ATP or ADP, the ATPase function, a second interaction site with DnaB, and ...
W, Messer, F, Blaesing, J, Majka, J, Nardmann, S, Schaper, A, Schmidt, H, Seitz, C, Speck, D, Tüngler, G, Wegrzyn, C, Weigel, M, Welzeck, J, Zakrzewska-Czerwinska   +12 more
openaire   +2 more sources

DnaA as a Transcription Regulator

2003
Publisher Summary This chapter reviews that DnaA is a transcription factor involved in the regulation of several E. Coli genes. DnaA can act as a repressor or it can be a transcriptional activator. DnaA might terminate transcription, provided two suitably located DnaA boxes mediate loop formation of the template.
Messer, W., Weigel, C.
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Transcription termination in the dnaA gene

Molecular and General Genetics MGG, 1991
The termination of transcription in the dnaA gene of E. coli was analyzed using transcriptional fusions to the galactokinase gene, S1 nuclease mapping and quantification of translation products by Western blots. The majority of transcripts originating from dnaA promoters terminated at several positions within a 200 bp region inside the dnaA reading ...
M, Wende, A, Quinones, L, Diederich, W R, Jueterbock, W, Messer   +4 more
openaire   +2 more sources

Directionality of DnaA protein/DNA interaction. Active orientation of the DnaA protein/dnaA box complex in transcription termination.

The EMBO Journal, 1989
The complex of DnaA protein with its 9 bp consensus binding site, the dnaA box 5'-TT(A/T)T(A/C)CA(A/C)A, blocks transcribing RNA polymerase. In a model system, the rate of transcription was monitored distal to the dnaA box 5'-TTTTCCACA by the expression of a reporter gene.
C, Schaefer, W, Messer
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Temperature-dependence of the DnaA–DNA interaction and its effect on the autoregulation of dnaA expression

Biochemical Journal, 2012
The DnaA protein is a key factor for the regulation of the timing and synchrony of initiation of bacterial DNA replication. The transcription of the dnaA gene in Escherichia coli is regulated by two promoters, dnaAP1 and dnaAP2. The region between these two promoters contains several DnaA-binding sites that have been shown to play an important role in ...
Saggioro, Chiara, Olliver, Anne, Sclavi, Bianca   +2 more
openaire   +3 more sources