Results 181 to 190 of about 8,858 (210)
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Cellular and Molecular Life Sciences (CMLS), 2003
DnaA protein binds specifically to a 9-base- pair motif called the DnaA box. Domain IV comprises 94 amino acid residues and is required for DNA binding. Using nuclear magnetic resonance analysis, we investigated the interaction between DnaA domain IV and both a DnaA box and a non-specific oligonucleotide that has a reduced affinity for DnaA. The 1H-15N
Y, Yoshida +6 more
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DnaA protein binds specifically to a 9-base- pair motif called the DnaA box. Domain IV comprises 94 amino acid residues and is required for DNA binding. Using nuclear magnetic resonance analysis, we investigated the interaction between DnaA domain IV and both a DnaA box and a non-specific oligonucleotide that has a reduced affinity for DnaA. The 1H-15N
Y, Yoshida +6 more
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Molecular Microbiology, 1991
SummaryDnaA protein (a trans‐acting element) and its binding sequence, DnaA‐box: (a cis‐acting element) are two elements essential for the initiation of chromosomal replication in Escherichia coli and other enteric bacteria. Recently these two elements have been found to be conserved in three Gram‐positive bacteria (Bacillus subtilis, Micrococcus ...
H, Yoshikawa, N, Ogasawara
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SummaryDnaA protein (a trans‐acting element) and its binding sequence, DnaA‐box: (a cis‐acting element) are two elements essential for the initiation of chromosomal replication in Escherichia coli and other enteric bacteria. Recently these two elements have been found to be conserved in three Gram‐positive bacteria (Bacillus subtilis, Micrococcus ...
H, Yoshikawa, N, Ogasawara
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Biochimie, 2001
Escherichia coli DnaA protein forms a multimeric complex at the chromosomal origin of replication (oriC), where a series of initiation reactions occurs and DNA polymerase III holoenzyme is loaded. The ATP-bound form of DnaA, which is active for initiation, is converted to the inactive ADP-bound form through interaction with the sliding clamp, the beta ...
Tsutomu Katayama, Kazuyuki Fujimitsu
exaly +2 more sources
Escherichia coli DnaA protein forms a multimeric complex at the chromosomal origin of replication (oriC), where a series of initiation reactions occurs and DNA polymerase III holoenzyme is loaded. The ATP-bound form of DnaA, which is active for initiation, is converted to the inactive ADP-bound form through interaction with the sliding clamp, the beta ...
Tsutomu Katayama, Kazuyuki Fujimitsu
exaly +2 more sources
Regulation of the dnaA product in Escherichia coli
Molecular and General Genetics MGG, 1977When an E. coli mutant (CRT46, dnaA46), thermosensitive in the initiation of DNA replication, grows at intermediate temperatures its DNA/mass ratio is somewhat lower than normal, but the cells possess an excess of initiation capacity, which can be expressed in the absence of protein synthesis and lead to the accumulation of anomalously high amounts of ...
F G, Hansen, K V, Rasmussen
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Trigger factor interacts with DnaA protein to stimulate its interaction with DnaA box
Korean Journal of Biological Sciences, 2003While screening proteins that interact with DnaA protein, the initiator protein for Escherichia coli chromosomal DNA replication, we found a 52‐kD sized protein which bound to DnaA protein in a salt‐dependent manner. This protein was identified as trigger factor, a ribosome‐associated peptidyl‐prolyl‐cis/trans isomerase with chaperone activity. Trigger
Yong Sun Lee +6 more
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Gene, 1992
We have previously shown that the dnaA gene and the DnaA-box region were conserved in bacteria representative of all three major branches of the eubacterial phylogenic tree: high G + C Gram+, low-G + C Gram+ and Gram-. In the present work, we determined the structure of the dnaA region of Mycoplasma capricolum and found that the dnaA gene and at least ...
M Q, Fujita, H, Yoshikawa, N, Ogasawara
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We have previously shown that the dnaA gene and the DnaA-box region were conserved in bacteria representative of all three major branches of the eubacterial phylogenic tree: high G + C Gram+, low-G + C Gram+ and Gram-. In the present work, we determined the structure of the dnaA region of Mycoplasma capricolum and found that the dnaA gene and at least ...
M Q, Fujita, H, Yoshikawa, N, Ogasawara
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Autorepression of the dnaA Gene of Escherichia Coli
1984The dnaA protein is essential for initiation of replication from the chromosomal origin, oriC (1), in Escherichia coli. It interacts with the RNA polymerase (2) in an early step in the initiation process (3). The dnaA protein is also involved in termination of transcription — dnaA(Ts) mutants show increased readthrough at the trp operon attenuator at ...
T, Atlung, E, Clausen, F G, Hansen
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The Role of DnaA-DnaA Box Interaction in Initiation of Chromosomal Replication in Bacillus subtilis
1992In bacteria, an autonomously replicating sequence (ars) was first isolated from the replication origin region of the Escherichia coli chromosome (Yasuda and Hirota, 1977) and was defined as oriC (origin of the chromosomal replication). Genetic and biochemical studies in E.
N. Ogasawara, S. Moriya, H. Yoshikawa
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The New biologist, 1991
The dnaA gene of Escherichia coli specifies a product that is a key element in the initiation of DNA replication. Expression of dnaA occurs from two promoters, 1P and 2P, which flank a DnaA protein binding site (DnaA box). In this paper we describe the effects of DnaA box mutations on transcription from the two promoters, measured with the use of dnaA ...
P, Polaczek, A, Wright
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The dnaA gene of Escherichia coli specifies a product that is a key element in the initiation of DNA replication. Expression of dnaA occurs from two promoters, 1P and 2P, which flank a DnaA protein binding site (DnaA box). In this paper we describe the effects of DnaA box mutations on transcription from the two promoters, measured with the use of dnaA ...
P, Polaczek, A, Wright
openaire +1 more source