Results 21 to 30 of about 254,386 (241)

Supercoiling Double-Stranded RNA [PDF]

Biophysical Journal, 2010
Through a novel "polymerase-stall" labeling procedure, we have successfully generated torsionally constrained molecules of double-stranded RNA (dsRNA). We have anchored these molecules within a magnetic tweezers apparatus, and by rotating the magnets, induced both positive and negative supercoils within these molecules.
Skinner, Gary M., Donkers, Serge P., Lipfert, Jan, Dekker, Nynke H.   +3 more
openaire   +1 more source

Efficiency of RNA interference is improved by knockdown of dsRNA nucleases in tephritid fruit flies [PDF]

Open Biology, 2019
RNA interference (RNAi) in insects is routinely used to ascertain gene function, but also has potential as a technology to control pest species. For some insects, such as beetles, ingestion of small quantities of double-stranded RNA (dsRNA) is able to ...
Alison Tayler, Daniel Heschuk, David Giesbrecht, Jae Yeon Park, Steve Whyard   +4 more
doaj   +1 more source

Tracking Cryptosporidium parvum by Sequence Analysis of Small Double-Stranded RNA

Emerging Infectious Diseases, 2001
We sequenced a 173-nucleotide fragment of the small double-stranded viruslike RNA of Cryptosporidium parvum isolates from 23 calves and 38 humans. Sequence diversity was detected at 17 sites.
Lihua Xiao, Josef Limor, Caryn Bern, Altaf A. Lal   +3 more
doaj   +1 more source

Targeting mitochondrial metabolites and nucleic acids as an anti-inflammatory strategy

Frontiers in Drug Discovery, 2023
Mitochondrial metabolites and their derivatives have been the focus of recent efforts to develop new anti-inflammatory therapeutics. The widely used therapeutic agents dimethyl fumarate (DMF) and metformin have anti-inflammatory properties and have been ...
Yukun Min, Luke A. J. O’Neill
doaj   +1 more source

LAST-seq: single-cell RNA sequencing by direct amplification of single-stranded RNA without prior reverse transcription and second-strand synthesis

Genome Biology, 2023
Existing single-cell RNA sequencing (scRNA-seq) methods rely on reverse transcription (RT) and second-strand synthesis (SSS) to convert single-stranded RNA into double-stranded DNA prior to amplification, with the limited RT/SSS efficiency compromising ...
Jun Lyu, Chongyi Chen
doaj   +1 more source

Interaction of PKR with single-stranded RNA

Scientific Reports, 2017
Although the antiviral kinase PKR was originally characterized as a double-stranded RNA activated enzyme it can be stimulated by RNAs containing limited secondary structure.
Christopher B. Mayo, James L. Cole
doaj   +1 more source

siRNA-like double-stranded RNAs are specifically protected against degradation in human cell extract. [PDF]

PLoS ONE, 2011
RNA interference (RNAi) is a set of intracellular pathways in eukaryotes that controls both exogenous and endogenous gene expression. The power of RNAi to knock down (silence) any gene of interest by the introduction of synthetic small-interfering (si ...
John A H Hoerter, Vishalakshi Krishnan, Troy A Lionberger, Nils G Walter   +3 more
doaj   +1 more source

A Rapid Method for Sequencing Double-Stranded RNAs Purified from Yeasts and the Identification of a Potent K1 Killer Toxin Isolated from Saccharomyces cerevisiae

Viruses, 2019
Mycoviruses infect a large number of diverse fungal species, but considering their prevalence, relatively few high-quality genome sequences have been determined.
Angela M. Crabtree, Emily A. Kizer, Samuel S. Hunter, James T. Van Leuven, Daniel D. New, Matthew W. Fagnan, Paul A. Rowley   +6 more
doaj   +1 more source

Arabidopsis thaliana endonuclease V is a ribonuclease specific for inosine-containing single-stranded RNA

Open Biology, 2021
Endonuclease V is highly conserved, both structurally and functionally, from bacteria to humans, and it cleaves the deoxyinosine-containing double-stranded DNA in Escherichia coli, whereas in Homo sapiens it catalyses the inosine-containing single ...
Megumi Endo, Jung In Kim, Narumi Aoki Shioi, Shigenori Iwai, Isao Kuraoka   +4 more
doaj   +1 more source

Double-Stranded RNA Resists Condensation

Physical Review Letters, 2011
Much attention has been focused on DNA condensation because of its fundamental biological importance. The recent discovery of new roles for RNA duplexes demands efficient packaging of double-stranded RNA for therapeutics. Here we report measurements of short DNA and RNA duplexes in the presence of trivalent ions.
Li, Li, Suzette A, Pabit, Steve P, Meisburger, Lois, Pollack   +3 more
openaire   +3 more sources