Results 51 to 60 of about 2,157,984 (211)

RNA Interference Method for Gene Function Analysis in the Japanese Rhinoceros Beetle Trypoxylus dichotomus

Bio-Protocol, 2022
In the Japanese rhinoceros beetle Trypoxylus dichotomus, various candidate genes required for a specific phenotype of interest are listed by next-generation sequencing analysis.
Kazuki Sakura, Shinichi Morita, Teruyuki Niimi   +2 more
doaj   +1 more source

Single‐Cell Transcriptomics and Metabolomics Reveal Glutamate Dehydrogenase as a Central Regulator of Nitrogen Metabolic Remodeling During Alkalinity Adaptation in Crustaceans

Advanced Science, EarlyView.
Single‐cell transcriptomics reveals the cellular response of Macrobrachium hainanense to carbonate alkalinity stress. Alkalinity stress impairs branchial ammonia excretion, induces mitochondrial dysfunction, and drives cell‐type‐specific changes in gills and hemocytes.
Yiting Jin, Zhimin Lv, Chao Bian, Qibin Yang, Na Zhou, Jianguang Qin, Shengming Sun   +6 more
wiley   +1 more source

Nucleoside‐Modified mRNA Encoding Alpha‐Galactosidase A Ameliorates Fabry Disease Phenotypes in Human IPSC‐Derived Cardiomyocytes

Advanced Science, EarlyView.
Human iPSC‐derived Fabry cardiomyocytes exhibited broad transcriptional dysregulation, apoptosis, mitochondrial dysfunction, impaired reactive oxygen species handling, altered contractility, and abnormal calcium transient decay, potentially mediated by phospholamban hyperphosphorylation.
Malte Juchem, Lea Oehlsen, Sedef Ersoy, Jia Li Ye, Natalie Weber, Elisa Mohr, Wilson Agyapong, Junqing Liu, Maximilian Fuchs, Ke Xiao, Christopher Jahn, Reto Eggenschwiler, Tobias Cantz, Julia Beimdiek, Falk F. R. Buettner, Theresia Kraft, Jan Hegermann, Ivonne J. Knorr, Lisa Ernst, Linda Feldbrügge, Resa Puffert, Kevin Schmidt, Christian Bär, Jeannine Hoepfner, Thomas Thum   +24 more
wiley   +1 more source

Dissipation of DvSnf7 Double-Stranded RNA in Brazilian Soils

Agricultural & Environmental Letters, 2019
Characterizing the environmental fate of DvSnf7 double-stranded RNA (dsRNA) produced by MON 87411 maize ( L.) in Brazilian soils is a critical component to a comprehensive environmental risk assessment.
Marcela Emanuele Scarso Joaquim, Gustavo Gross Belchior, Marcia Ometto de Mello Alves José, Fatima Zapata, Changjian Jiang, Joshua Fischer, Geraldo Ubirajara Berger   +6 more
doaj   +1 more source

Advances in nanocarriers to improve the stability of dsRNA in the environment

Frontiers in Bioengineering and Biotechnology, 2022
RNAi technology, known as a revolutionary technology in the history of pesticides, has been identified as a very promising novel approach for crop protection, which is of great significance for achieving the sustainable agricultural development of the ...
Wenling Yang, Baitao Wang, Gao Lei, Guocan Chen, Dehai Liu   +4 more
doaj   +1 more source

An Effector TaCRVP From Tuta Absoluta Oral Secretion Impairs H2O2‐Mediated Plant Defense by Targeting Tomato SlCAT2

Advanced Science, EarlyView.
The Tuta absoluta salivary effector TaCRVP subverts tomato immunity by hijacking host catalase SlCAT2. Through a sophisticated dual mechanism, TaCRVP simultaneously promotes SlCAT2 tetramerization for activation and blocks its SlAdBiL‐mediated degradation for stabilization.
Jianquan Yan, Youdan Zhang, Zhujun Wang, Huatao Tang, Huiping Liu, Zi Chen, Shuai Liu, Jiayi Shi, Lu Peng, Youming Hou   +9 more
wiley   +1 more source

RNAi in Insect Physiology: Unlocking Mechanisms and Pioneering Sustainable Pest Control

Insects
The advent of RNA interference (RNAi) in 1998, marked by the discovery of gene silencing triggered by double-stranded RNA (dsRNA) in nematodes, opened a transformative chapter in molecular biology [...]
Jisheng Liu, Luc Swevers
doaj   +1 more source

TP53 Loss Elevates NF‐κB‐IFN‐β‐MHC‐Ia Signaling to Promote NK Cell Resistance in Osteosarcoma

Advanced Science, EarlyView.
TP53 loss in a transforming or osteosarcoma cell promotes cytosolic DNA accumulation, activating NF‐κB‐dependent IFN‐β production. Autocrine IFN‐β signaling increases cell‐surface HLA‐Ia expression, strengthens inhibitory KIR signaling in natural killer cells, and thereby enables the affected cell to evade NK cell‐mediated cytotoxicity.
Guihui Qin, Cheung Kwan Yeung, Ye Yi, Dejin Zheng, Miaoman Ye, Chi‐Chong Chio, Jinjie Wu, Siyi Fu, Chu‐Xia Deng, Ren‐He Xu   +9 more
wiley   +1 more source

Investigation of RNA-induced gene silencing in the fission yeast Schizosaccharomyces pombe [PDF]

, 2007
PhDRNA interference (RNAi) is a gene regulating system to which can silence genes at both transcriptional and post-transcriptional levels. In this thesis, a ura4-based RNAi selective assay was developed in the fission yeast Schizosaccharomyces pombe ...
Wang, Huan
core   +4 more sources

Assessment of In Vitro Nano‐Cytotoxicity Through Fluorescence Microscopy via Neural Network‐Based Live/Dead Cell Classification

Advanced Intelligent Systems, EarlyView.
FluoAI is a two‐stage, label‐free method for quantifying nanomaterial‐induced cytotoxicity from conventional fluorescence microscopy images. Mask R‐CNN segmentation and DenseNet‐121 classification provide rapid live/dead classification matching human accuracy.
Ugur C. Topkiran, Ali Gasimli, Lal Durmaz, Diya Vashani, Himish Paul, Giridhar R. Akkaraju, Anton V. Naumov   +6 more
wiley   +1 more source