Results 61 to 70 of about 2,157,984 (211)

Duck Orbivirus and Egg Production: A Newly Identified Duck Orbivirus Is the Etiological Agent of Egg Production Decline in Chinese Breeder Ducks

Animal Research and One Health, EarlyView.
Field samples from breeder duck farms with egg‐production decline were subjected to viral detection, genome sequencing, and virus isolation. Experimental infection demonstrated that duck orbivirus (DORV) causes a significant and persistent reduction in egg production in breeder ducks.
Bing Li, Jianhua Wang, Xuezhi Cui, Xinxing Chu, You Wang, Dongmin Hao, Mingqing Xu, Haixia Chang, Huihui Li, Mingtian Mao, Mian Wu, Chengguang Lu, Jing Tang, Suyun Liang, Zhanbao Guo, Zhengkui Zhou, Youxiang Diao, Shuisheng Hou, Yi Tang   +18 more
wiley   +1 more source

The Effect of Oral Administration of dsRNA on Viral Replication and Mortality in Bombus terrestris

Viruses, 2015
Israeli acute paralysis virus (IAPV), a single-stranded RNA virus, has a worldwide distribution and affects honeybees as well as other important pollinators.
Niels Piot, Simon Snoeck, Maarten Vanlede, Guy Smagghe, Ivan Meeus   +4 more
doaj   +1 more source

Enhancing the Secretion Systems: Genetic Engineering of Super Bioagents for Effective Plant Disease Control

Biotechnology and Bioengineering, EarlyView.
Genetic engineering (GE) overcomes BCA limits, enabling robust, scalable crop protection. “Super Bioagents” (SBs) use optimized SSs to enhance bioactive molecule delivery. Optimizing SSs enhance precise, sustainable effector deployment for stable disease suppression. SBs use microbiome‐informed design for scalable, next‐gen sustainable crop protection.
Michael Dare Asemoloye
wiley   +1 more source

Universal and quantitative detection of double-stranded RNAs as a signature of pan-virus infections using a luciferase-based biosensor

Journal of Biological Engineering
Background Infections with various RNA viruses and certain DNA viruses may produce double-stranded RNA (dsRNA) during replication, which trigger host innate immune responses.
Michihito Sasaki, Eri Fujii, Satoko Sasaki, Takuma Ariizumi, Kei Konishi, Akihiko Sato, William W. Hall, Hirofumi Sawa, Yasuko Orba   +8 more
doaj   +1 more source

Development of an RNA Nanostructure for Effective Botrytis cinerea Control through Spray-Induced Gene Silencing without an Extra Nanocarrier

Journal of Fungi
Spray-induced gene silencing represents an eco-friendly approach for crop protection through the use of double-stranded RNA (dsRNA) to activate the RNA interference (RNAi) pathway, thereby silencing crucial genes in pathogens.
Fangli Wu, Ling Yan, Xiayang Zhao, Chongrun Lv, Weibo Jin   +4 more
doaj   +1 more source

Process Analytical Technology (PAT) for Continuous Purification of mRNA via Precipitation and Tangential Flow Filtration

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Continuous downstream processing of messenger ribonucleic acid (mRNA) therapeutics requires purification strategies that are scalable, robust, and capable of removing product‐ and process‐related impurities from in vitro transcription mixtures.
Rajan R. Bhawnani, Maria del Carme Pons Royo, Rohan Kadambi, Andrew Hatas, Steve Burcat, Tyler Arnold, Hasan Al‐Mahayni, Richard D. Braatz, Allan S. Myerson   +8 more
wiley   +1 more source

Single-molecule analysis of processive double-stranded RNA cleavage by Drosophila Dicer-2

Nature Communications, 2021
Fly Dicer-2 is thought to use two distinct – processive or distributive – modes of cleavage by distinguishing the terminal structures of double-stranded RNA (dsRNA) substrates with the help of its cofactor LoquaciousPD (Loqs-PD). Here the authors show by
Masahiro Naganuma, Hisashi Tadakuma, Yukihide Tomari   +2 more
doaj   +1 more source

The influence of viral RNA secondary structure on interactions with innate host cell defences [PDF]

, 2013
RNA viruses infecting vertebrates differ fundamentally in their ability to establish persistent infections with markedly different patterns of transmission, disease mechanisms and evolutionary relationships with their hosts.
Blundell, Richard, Richard Blundell, Nora McFadden, Joris J. Maarleveld, Maarleveld, Joris J, Evans, David J, Simmonds, Peter, Jeroen Witteveldt, Maarleveld, Joris J., David J. Evans, McFadden, Nora, Peter Simmonds, Evans, David J., Witteveldt, Jeroen   +13 more
core   +1 more source

Engineering mRNA‐LNP Medicines for the Ageing Brain: Opportunities and Challenges for Neurodegenerative Diseases

Exploration, EarlyView.
This review highlights recent advances in engineering messenger RNA (mRNA)‐lipid nanoparticles (LNPs) to cross the ageing blood–brain barrier and target neurodegenerative diseases. It outlines design principles, delivery routes, and translational challenges, charting a roadmap towards clinical application of mRNA‐LNP therapeutics for neurodegenerative ...
Abdel Ali Belaidi, Denzil L. Furtado, Francesca Alves, Rebecca M. Nisbet, Scott Ayton, Ashley I. Bush   +5 more
wiley   +1 more source

Targeting UDP‐Bta06664 in Bemisia tabaci: A novel strategy to reduce tomato yellow leaf curl virus transmission by impairing insect vector feeding

New Plant Protection, EarlyView.
Tomato yellow leaf curl virus upregulates the Bemisia tabaci UDP‐glycosyltransferase gene UDP‐Bta06664. RNA interference (RNAi) silencing of this gene disrupts phloem feeding—delaying E1 and shortening E2—and reduces both virus acquisition and inoculation, highlighting its potential as an RNAi target.
Zeyu Wan, Liping Huang, Zhanhong Zhang, Xuguo Zhou, Yong Liu, Xiaobin Shi   +5 more
wiley   +1 more source