Results 151 to 160 of about 15,397 (180)

Drosha cuts the tethers of myelopoiesis

Nature Immunology, 2015
The RNA endonuclease Drosha is required for myelopoiesis by its direct cleavage of stem-loop structures in mRNAs encoding Myl9 and Todr1.
Annemarthe G van der Veen, Pierre V Maillard, Caetano Reis e Sousa   +2 more
openaire   +3 more sources

Impaired expression of Drosha in breast cancer

Breast Disease, 2017
BACKGROUND: Impaired miRNAs processing pathway is one interesting scenario for global downregulation of the miRNAome in various types of malignancy. We previously reported that DGCR8 and Dicer genes dysregulated in patients with breast cancer. OBJECTIVE: To evaluate the expression pattern of Drosha in patients with breast cancer.
Ali Akbar, Poursadegh Zonouzi, Mohammad, Shekari, Azim, Nejatizadeh, Samira, Shakerizadeh, Hedieh, Fardmanesh, Ahmad, Poursadegh Zonouzi, Mohammad, Rahmati-Yamchi, Majid, Tozihi   +7 more
openaire   +2 more sources

Dicer and Drosha expression in patients with nephrotic syndrome

BioFactors, 2020
AbstractPodocytes play an essential role in the regulation of glomerular filtration and the appropriate function of the kidney. Podocytes injury is involved in the pathogenesis of nephrotic syndrome (NS), a common renal glomerulus dysfunction characterized by proteinuria.
Seyyedeh Mina Hejazian, Yalda Rahbar Saadat, Zahra Bahmanpour, Seyed Mahdi Hosseiniyan Khatibi, Mohammadreza Ardalan, Sepideh Zununi Vahed   +5 more
openaire   +2 more sources

Review: Non-canonical role of Drosha ribonuclease III

International Journal of Biological Macromolecules, 2023
The typical function of Drosha is participating in cleaving pri-miRNA, the initial step of miRNA biogenesis, in the nucleus. Since Drosha has a double-stranded RNA-binding domain and two RNase III domains, when it binds and/or cleaves other RNA species other than pri-miRNA, Drosha is able to induce a variety of novel biological effects.
Xuanshuo, Wei, Jin, Tang, Chuwen, Lin, Xuan, Jiang   +3 more
exaly   +3 more sources

A bioinformatics approach for investigating the determinants of Drosha processing

13th IEEE International Conference on BioInformatics and BioEngineering, 2013
We use a bioinformatics approach to search for the biological features that determine the cleavage site of the Microprocessor complex (or Drosha) within known miRNA hairpins. Towards this goal, we employ a previously developed methodology, termed DuplexSVM, which can accurately identify the four ends of a miRNA:miRNA* duplex.
Nestoras Karathanasis, Ioannis Tsamardinos, Panayiota Poirazi   +2 more
openaire   +1 more source

In Vitro and In Vivo Assays for the Activity of Drosha Complex

2007
MicroRNA (miRNA) genes are transcribed into long primary transcripts (pri-miRNAs) that get processed into mature miRNAs of about 22 nt in length by two different ribonuclease (RNase) III enzymes, Drosha and Dicer. Various experimental protocols have been developed and modified for genetic and biochemical analyses for microRNA processing.
Yoontae, Lee, V Narry, Kim
openaire   +2 more sources

Cytoplasmic and nuclear DROSHA in human villous trophoblasts

Journal of Reproductive Immunology
In villous trophoblasts, DROSHA is a key ribonuclease III enzyme that processes pri-microRNAs (pri-miRNAs) into pre-miRNAs at the placenta-specific, chromosome 19 miRNA cluster (C19MC) locus. However, little is known of its other functions. We performed formaldehyde crosslinking, immunoprecipitation, and sequencing (fCLIP-seq) analysis of terminal ...
Syunya, Noguchi, Sadayuki, Ohkura, Yasuyuki, Negishi, Shohei, Tozawa, Takami, Takizawa, Rimpei, Morita, Hironori, Takahashi, Akihide, Ohkuchi, Toshihiro, Takizawa   +8 more
openaire   +2 more sources

The nuclear RNase III Drosha initiates microRNA processing

Nature, 2003
Hundreds of small RNAs of approximately 22 nucleotides, collectively named microRNAs (miRNAs), have been discovered recently in animals and plants. Although their functions are being unravelled, their mechanism of biogenesis remains poorly understood.
Yoontae, Lee, Chiyoung, Ahn, Jinju, Han, Hyounjeong, Choi, Jaekwang, Kim, Jeongbin, Yim, Junho, Lee, Patrick, Provost, Olof, Rådmark, Sunyoung, Kim, V Narry, Kim   +10 more
openaire   +2 more sources

Expression of Dicer and Drosha in triple-negative breast cancer

Journal of Clinical Pathology, 2012
Aims Dicer and Drosha are components of the miRNA-producing machinery and their altered expression may play a role in cancer progression. The main purpose of this study was a detailed investigation of Dicer and Drosha expression and localisation in triple-negative breast cancers.
Passon N, Gerometta A, Puppin C, Lavarone E, PUGLISI, Fabio, TELL, Gianluca, DI LORETO, Carla, DAMANTE, Giuseppe   +7 more
openaire   +2 more sources

Cloning, expression, and characterization of the zebrafish Dicer and Drosha enzymes

Biochemical and Biophysical Research Communications, 2019
The biogenesis of animal microRNAs (miRNAs) involves transcription followed by a series of processing steps, with Drosha and Dicer being two key enzymes that cleave primary miRNA (pri-miRNA) and precursor miRNA (pre-miRNA) transcripts, respectively. While human and fly Dicer and human Drosha are well studied, their homologs in other organisms have not ...
Peng, Li, Qiuhuan, Tian, Min, Hu, Wenjing, Li, Xiaoxiao, Zhang, Yan, Zeng   +5 more
openaire   +2 more sources