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A bioinformatics approach for investigating the determinants of Drosha processing
13th IEEE International Conference on BioInformatics and BioEngineering, 2013We use a bioinformatics approach to search for the biological features that determine the cleavage site of the Microprocessor complex (or Drosha) within known miRNA hairpins. Towards this goal, we employ a previously developed methodology, termed DuplexSVM, which can accurately identify the four ends of a miRNA:miRNA* duplex.
Nestoras Karathanasis +2 more
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In Vitro and In Vivo Assays for the Activity of Drosha Complex
2007MicroRNA (miRNA) genes are transcribed into long primary transcripts (pri-miRNAs) that get processed into mature miRNAs of about 22 nt in length by two different ribonuclease (RNase) III enzymes, Drosha and Dicer. Various experimental protocols have been developed and modified for genetic and biochemical analyses for microRNA processing.
Yoontae, Lee, V Narry, Kim
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The nuclear RNase III Drosha initiates microRNA processing
Nature, 2003Hundreds of small RNAs of approximately 22 nucleotides, collectively named microRNAs (miRNAs), have been discovered recently in animals and plants. Although their functions are being unravelled, their mechanism of biogenesis remains poorly understood.
Yoontae, Lee +10 more
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Expression of Dicer and Drosha in triple-negative breast cancer
Journal of Clinical Pathology, 2012Aims Dicer and Drosha are components of the miRNA-producing machinery and their altered expression may play a role in cancer progression. The main purpose of this study was a detailed investigation of Dicer and Drosha expression and localisation in triple-negative breast cancers.
Passon N +7 more
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Cytoplasmic and nuclear DROSHA in human villous trophoblasts
Journal of Reproductive ImmunologyIn villous trophoblasts, DROSHA is a key ribonuclease III enzyme that processes pri-microRNAs (pri-miRNAs) into pre-miRNAs at the placenta-specific, chromosome 19 miRNA cluster (C19MC) locus. However, little is known of its other functions. We performed formaldehyde crosslinking, immunoprecipitation, and sequencing (fCLIP-seq) analysis of terminal ...
Syunya, Noguchi +8 more
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Cloning, expression, and characterization of the zebrafish Dicer and Drosha enzymes
Biochemical and Biophysical Research Communications, 2019The biogenesis of animal microRNAs (miRNAs) involves transcription followed by a series of processing steps, with Drosha and Dicer being two key enzymes that cleave primary miRNA (pri-miRNA) and precursor miRNA (pre-miRNA) transcripts, respectively. While human and fly Dicer and human Drosha are well studied, their homologs in other organisms have not ...
Peng, Li +5 more
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Drosha and DGCR8 in MicroRNA Biogenesis
2012Abstract Drosha and DGCR8 (also called Pasha), together known as the Microprocessor complex, are responsible for cleaving canonical microRNA primary transcripts (pri-miRNAs) to produce microRNA precursors (pre-miRNAs) in animals. This specific cleavage serves as the entrance to the microRNA (miRNA) maturation pathway.
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Cryo-EM Structures of Human Drosha and DGCR8 in Complex with Primary MicroRNA
Molecular Cell, 2020Kaiming Zhang +2 more
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