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A polymer/detergent formulation improves dsRNA penetration through the body wall and RNAi-induced mortality in the soybean aphid Aphis glycines.

Pest Management Science, 2019
BACKGROUND It is difficult to efficiently silence gene expression in some insects, probably because of the degradation of dsRNA by enzymes present in the gut and hemolymph post-oral feeding or body injecting of dsRNA.
Yang Zheng   +6 more
semanticscholar   +1 more source

Packaging in dsRNA Viruses

2011
Several families of viruses have segmented genomes with 3-12 chromosomes. They are capable of packaging these segments in a precise manner so that each virus particle contains one each of the genomic segments. The Cystoviridae are a family of bacteriophages that contain three genomic segments of dsRNA.
openaire   +2 more sources

dsRNA Inhibits Protein Expression

Science's STKE, 2000
The ability of double-stranded RNA (dsRNA) to block gene expression is termed RNA interference (RNAi). RNAi has been identified in Drosophila, Caenorhabditis , and other organisms as a way to suppress transposition and to silence endogenous genes.
openaire   +2 more sources

dsRNA Genetic Elements

2001
RNAi and Cosuppression: Double-Stranded RNA as an Agent of Sequence-Specific Genetic Silencing in Animals and Plants, Jeffrey A. Norman and Mary K. Montgomery The Double-Stranded RNA-Activated Protein Kinase PKR, Randal J. Kaufman The Double-Stranded RNA Viruses of Saccharomyces Cerevisiae, Reed B. Wickner, Juan Carlos Ribas, and Anjanette Searfoss The
openaire   +1 more source

TLR3 Tolls for dsRNA

Science's STKE, 2001
Members of the Toll-like receptor (TLR) family appear to recognize molecular patterns found on pathogens. Alexopoulou et al. have found that double-stranded RNA (dsRNA), which can be associate with viral infection, is recognized by TLR3. DsRNA-treated HEK293 cells that expressed TLR3 showed increased activity of the
openaire   +2 more sources

dsRNA Delivery Service

Science's STKE, 2006
CD14 is a glycosylphosphatidylinositol (GPI)-anchored pattern recognition receptor that is involved in activation of Toll-like receptors (TLRs) at the cell surface (see Finberg and Kurt-Jones). For example, it binds to lipopolysaccharide (LPS) and viruses to present these foreign agents to TLR4 and TLR2, respectively. Lee et al.
openaire   +1 more source

dsRNA in Diagnosis

2019
This chapter describes the practical value for attempting to detect and describes the double-stranded (dsRNAs) lies in the ease with which they can be isolated and characterized. Most diagnostic laboratories recognize the value of less specific tests which can be used in addition to specific tests in the early testing stages, especially when the ...
openaire   +1 more source

Contributions of dsRNases to differential RNAi efficiencies between the injection and oral delivery of dsRNA in Locusta migratoria.

Pest Management Science, 2019
BACKGROUND The efficiency of RNA interference (RNAi) varies considerably among different insect species, and there is growing evidence to suggest that degradation of double-stranded (dsRNA) prior to uptake is an important factor that limits the ...
Huifang Song   +9 more
semanticscholar   +1 more source

A β2-tubulin dsRNA derived from Fusarium asiaticum confers plant resistance to multiple phytopathogens and reduces fungicide resistance.

Pesticide Biochemistry and Physiology, 2019
Crops are attacked by a large number of pathogens which are responsible for an approximately 30% loss in global crop production at pre- and post-harvest levels.
Kai-xin Gu   +7 more
semanticscholar   +1 more source

Topical dsRNA delivery induces gene silencing and mortality in the pea aphid.

Pest Management Science, 2019
BACKGROUND With the growing number of available aphid genomes and transcriptomes, an efficient and easy-to-adapt tool for gene function study is urgently required.
J. Niu   +13 more
semanticscholar   +1 more source

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