Results 341 to 350 of about 114,219 (358)

The competitive landscape of the dsRNA world

Molecular Cell
The ability to sense and respond to infection is essential for life. Viral infection produces double-stranded RNAs (dsRNAs) that are sensed by proteins that recognize the structure of dsRNA. This structure-based recognition of viral dsRNA allows dsRNA sensors to recognize infection by many viruses, but it comes at a cost-the dsRNA sensors cannot always
Kyle A, Cottrell, Ryan J, Andrews, Brenda L, Bass   +2 more
openaire   +2 more sources

Liposome encapsulation and EDTA formulation of dsRNA targeting essential genes increase oral RNAi-caused mortality in the Neotropical stink bug Euschistus heros.

Pest Management Science, 2018
BACKGROUND The Neotropical stink bug Euschistus heros is a major pest in soybean fields. Development of highly species-specific pesticides based on RNA interference (RNAi) could provide a new sustainable and environmentally friendly control strategy ...
N. Castellanos, G. Smagghe, Rohit Sharma, E. E. Oliveira, O. Christiaens   +4 more
semanticscholar   +1 more source

Arrest of Cancer Cell Proliferation by dsRNAs

Annals of the New York Academy of Sciences, 2006
Abstract:  Inhibition of c‐myc and N‐myc genes by dsRNAs in carcinoma and neuroblastoma cells was investigated. siRNA‐Ex3 targeted to the third exon of c‐myc gene was found to decrease the level of c‐myc but not N‐myc mRNA and decrease the rate or even arrest proliferation of c‐myc overexpressing cell lines KB‐3‐1 and SK‐N‐MC.
Al`Bina V. Vladimirova, Elena L. Chernolovskaya, Valentin V. Vlassov, Tatyana O. Kabilova   +3 more
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Preparation of dsRNAs for RNAi by In Vitro Transcription

Cold Spring Harbor Protocols, 2019
This is a simple and widely used method for the preparation of dsRNA using a polymerase chain reaction (PCR) template-based, in vitro transcription reaction. The resulting dsRNA is then used to trigger RNAi in an appropriate cell or organism.
Phillip D. Zamore, Chengjian Li
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A New Method to Isolate Total dsRNA

2014
When a diseased plant is suspected to be infected with unknown viruses, the approach of isolating double-stranded RNA (dsRNA) from diseased tissues and analyzing the sequence has been useful for detecting the viruses. This procedure owes its success to the majority of plant pathogenic viruses being RNA viruses, which accumulate dsRNAs as copies of ...
Kappei Kobayashi, Go Atsumi, Ken-Taro Sekine   +2 more
openaire   +3 more sources

Preparation of dsRNA for Microinjection Experiments in Mouse

Cold Spring Harbor Protocols, 2009
INTRODUCTIONRNA interference (RNAi) is a suitable method for sequence-specific post-transcriptional gene silencing for a number of model systems. The protocol presented here is designed for the preparation of microgram amounts of double-stranded RNA (dsRNA) for microinjection experiments in mouse oocytes and early embryos.
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Identification of Mycoviruses by dsRNA Extraction

Mycoviruses exist in all major groups of fungi. With the continuous development of science and technology, the methods of studying viruses are constantly updated, and progressively mycoviruses have been discovered where most of these viruses are RNA viruses.
Yanfei, Wang, Clement, Nzabanita, Lihua, Guo   +2 more
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The dsRNA Viridae and their catalytic capsids

Nature Structural Biology, 2002
dsRNA viruses share similar replicative strategies and the same unusual inner capsid architecture. The fold of the inner capsid protein is not conserved, however.
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Choosing the Sequence of dsRNA for RNAi in Mouse

Cold Spring Harbor Protocols, 2009
INTRODUCTIONRNA interference (RNAi) is a suitable method for sequence-specific post-transcriptional gene silencing for a number of model systems. Here, we describe selection of the target sequence for efficient RNAi knockdown in mouse.
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Injection of dsRNA and Electroporation in Avian Embryos

Cold Spring Harbor Protocols, 2006
INTRODUCTIONThis protocol describes a method for in ovo transfection of avian embryos with double-stranded RNA (dsRNA). The dsRNA is injected into the spinal cord of the embryo. Subsequent electroporation facilitates the cellular uptake of the dsRNA molecules.
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