Results 71 to 80 of about 73,913 (251)
Chitosan–Tripolyphosphate Nanoparticles Enhance Foliar dsRNA Delivery and Suppress Turnip Crinkle Virus in Arabidopsis [PDF]
The Plant Pathology JournalTopically applied double-stranded RNA (dsRNA) can trigger antiviral RNA interference in plants, but naked dsRNA is unstable and poorly taken up after foliar application. Here, we developed an eco-friendly chitosan–tripolyphosphate (CS-TPP) nanocarrier to
Min-Jae Kim +2 more
doaj +1 more source
Biotechnology and Bioengineering, EarlyView.ABSTRACT Continuous downstream processing of messenger ribonucleic acid (mRNA) therapeutics requires purification strategies that are scalable, robust, and capable of removing product‐ and process‐related impurities from in vitro transcription mixtures.
Rajan R. Bhawnani +8 more
wiley +1 more source
Recognition of Double-Stranded RNA and Regulation of Interferon Pathway by Toll-Like Receptor 10
Frontiers in Immunology, 2018 Toll-like receptor (TLR)-10 remains an orphan receptor without well-characterized ligands or functions. Here, we reveal that TLR10 is predominantly localized to endosomes and binds dsRNA in vitro at endosomal pH, suggesting that dsRNA is a ligand of ...
Suki Man-Yan Lee +6 more
doaj +1 more source
Colorimetric assay to detect interactions of NS4 with dsRNA.
, 2013 The graph shows colorimetric OD readings plotted against concentrations of dsRNA. Increasing concentrations (from 1 to 640 ng) of a biotinylated dsRNA were bound to wells coated with streptavidin. BTV NS4 or GIV NS4 were added to the wells in triplicate.
Mourad Belhouchet (321963) +5 more
core +1 more source
Deep Underground Science and Engineering, EarlyView.This study demonstrates that the ultra‐low background radiation environment in deep underground laboratories significantly inhibits the proliferation of both normal nasopharyngeal epithelial cells (HNEPC) and nasopharyngeal carcinoma cells (HNE‐1) cells, with malignant cells exhibiting higher sensitivity.
Yuhao Zou +15 more
wiley +1 more source
Shengwu huaxue yu shengwu wuli jinzhanT7 RNA polymerase (T7 RNAP) is one of the simplest known RNA polymerases. Its unique structural features make it a critical model for studying the mechanisms of RNA synthesis.
NING Wei-Chen +6 more
doaj +1 more source
Exploration, EarlyView.This review highlights recent advances in engineering messenger RNA (mRNA)‐lipid nanoparticles (LNPs) to cross the ageing blood–brain barrier and target neurodegenerative diseases. It outlines design principles, delivery routes, and translational challenges, charting a roadmap towards clinical application of mRNA‐LNP therapeutics for neurodegenerative ...
Abdel Ali Belaidi +5 more
wiley +1 more source
Frontiers in Cellular and Infection Microbiology, 2018 An important step in the initiation of the innate immune response to virus infection is the recognition of non-self, viral RNA, including double-stranded RNA (dsRNA), by cytoplasmic pattern recognition receptors (PRRs).
Elizabeth J. Mateer +2 more
doaj +1 more source
Electrophorosis of dsRNA-CarE.
, 2014 M: Molecular weight marker 5000; 1, 2, 3: dsRNA-CarE. Three groups of dsRNA-CarE were dissolved in 50 µL diethypyrocarbonate (DEPC)-treated water, analyzed with gel electrophoresis (1% agarose).
Xin-Rui Yu (619057) +4 more
core +1 more source
iMetaMed, EarlyView.This review systematically summarizes the central role of cysteine peptidases in modulating antigen‐presenting cell (APC) function and antitumor immune responses, highlighting their critical significance in overcoming tumor immune evasion. Current research has established that cysteine peptidase subtypes, including cathepsins B, S, L, and X, exert ...
Yimao Wu +7 more
wiley +1 more source