Results 81 to 90 of about 119,642 (302)
Microbes and Environments, 2021 Fragmented and primer ligated dsRNA sequencing (FLDS) is a sequencing method applicable to long double-stranded RNA (dsRNA) that enables the complete genome sequencing of both double- and single-stranded RNA viruses. However, the application of this method on a low amount of dsRNA has been hindered by adaptor dimer formation during cDNA amplification ...
Hirai, Miho +5 more
openaire +3 more sources
Depletion of the RNA‐Editing Enzyme ADAR1 Invigorates the Antitumor Immunity of NK Cells
Advanced Science, EarlyView.ADAR1 is upregulated in NK cells from melanoma patients, impairing their function. Its loss enhances NK cell tumor infiltration and cytotoxicity in vitro and in vivo. Mechanistically, ADAR1 deficiency destabilizes CD38 mRNA to reduce its expression, thereby increasing NK cell mobility and killing, which nominates it as a therapeutic target for NK cell ...
Shuhan Chen +11 more
wiley +1 more source
Silencing of two insulin receptor genes disrupts nymph-adult transition of alate brown citrus aphid [PDF]
, 2017 Insulin receptors play key roles in growth, development, and polymorphism in insects. Here, we report two insulin receptor genes (AcInR1 and AcInR2) from the brown citrus aphid, Aphis (Toxoptera) citricidus.
Ding, Bi-Yue +7 more
core +2 more sources
Plant Genetic Engineering: Technological Pathways, Application Scenarios, and Future Directions
Advanced Science, EarlyView.This review maps the fast‐evolving landscape of plant genetic engineering, linking enabling platforms with trait‐focused applications in architecture optimization, stress resilience, yield improvement, and quality enhancement. It highlights how genome editing, transgenic strategies, and emerging multi‐gene approaches reshape breeding pipelines, while ...
Peilin Wang +4 more
wiley +1 more source
Barriers to Efficient Foliar Uptake of dsRNA and Molecular Barriers to dsRNA Activity in Plant Cells
Frontiers in Plant Science, 2020 Foliar application of dsRNA to elicit an RNA interference (RNAi) response is currently under consideration as a crop protection strategy. To access the RNAi machinery of a plant, foliarly applied dsRNAs must traverse the plant cuticle, avoid nuclease ...
Michael J. Bennett +3 more
semanticscholar +1 more source
Suppression of Argonaute 2 Transcript Levels in Du182A Cells [PDF]
, 2018 RNA interference (RNAi) uses double-stranded RNA (dsRNA) molecules to degrade and suppress the transcript level of a complementary mRNA target1.
Featherston, Kyah +2 more
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Enhanced Intracellular Stability and Translation Efficiency of mRNA Drugs by a 2‐arm mRNA Platform
Advanced Science, EarlyView.We constructed a 2‐arm mRNA, characterized by a unique topology formed through the dimerization of two mRNA 3’ tails. The 2‐arm mRNA improves 3’ tail stability and resistance to nuclease degradation, resulting in an intracellular half‐life of up to 65 h. This method substantially enhances the translation capacity of mRNA drugs.
Xucong Teng +5 more
wiley +1 more source
Analysis of Expression Patterns of PcABCG5 Gene in Penaeus chinensis Under Saline-Alkali Stress
Progress in Fishery SciencesPenaeus chinensis is one of the most economically important species in northern China. Strong market demand requires the expansion of aquaculture production capacity of P. chinensis.
Yalun LI +5 more
doaj +1 more source
PLoS ONE, 2013 Rhinovirus (RV) infections cause exacerbations and development of severe asthma highlighting the importance of antiviral interferon (IFN) defence by airway cells.
Jenny Calvén +2 more
doaj +1 more source
Next-generation sequencing of dsRNA is greatly improved by treatment with the inexpensive denaturing reagent DMSO. [PDF]
, 2019 dsRNA is the genetic material of important viruses and a key component of RNA interference-based immunity in eukaryotes. Previous studies have noted difficulties in determining the sequence of dsRNA molecules that have affected studies of immune function
Delwart, Eric +2 more
core