Results 101 to 110 of about 68,502 (270)

Development of Biolayer Interferometry (BLI)-Based Double-Stranded RNA Detection Method with Application in mRNA-Based Therapeutics and Vaccines

Pharmaceutics
Background: In vitro-transcribed (IVT) mRNA has been established as a promising platform for therapeutics and vaccine development. Double-stranded RNA (dsRNA) is a major impurity of IVT mRNA and can trigger unfavored immune responses, potentially causing
Dharia Sara Silas, Bindiya Juneja, Keerat Kaur, Muralikrishna Narayanareddy Gari, Yingjian You, Youmi Moon, Yizhuo Chen, Srishti Arora, Johanna Hansen, Kathir Muthusamy, Yue Fu, Nisha Palackal, Erica A. Pyles   +12 more
doaj   +1 more source

Reporters transiently transfected into mammalian cells are highly sensitive to translational repression induced by dsRNA expression.

PLoS ONE, 2014
In mammals, double-stranded RNA (dsRNA) can mediate sequence-specific RNA interference, activate sequence-independent interferon response, or undergo RNA editing by adenosine deaminases. We showed that long hairpin dsRNA expression had negligible effects
Jana Nejepinska, Radek Malik, Susan Wagner, Petr Svoboda   +3 more
doaj   +1 more source

dsRNA and dsRNA/LyoVec upregulate expression of the RLRs RIG-I and MDA5 in BSMCs.

, 2013
BSMC were treated with dsRNA or the RLR ligand dsRNA/LyoVec (dsRNA/LV) for 24 h. mRNA (A, C) and protein (B, D) expression of RIG-I (A, B) and MDA5 (C, D) was increased concentration-dependently.
Lena Uller (83922), Jenny Calvén (407978), Yuliana Yudina (234201)   +2 more
core   +1 more source

dsRNA subtomogram processing.

, 2023
Workflow used in subtomogram alignment, classification and averaging to compute the five averages of dsRNA shown in Fig 1C–1G. (TIF)
Lars-Anders Carlson (236630), Timothée Laurent (16496619)   +1 more
core   +1 more source

RNA degradomics and proteomics reveal the mechanism of dsProsβ1‐mediated proteasome targeting in Psylliodes chrysocephala

Pest Management Science, EarlyView.
RNA degradomics revealed dsProsβ1‐derived siRNA‐mediated mRNA cleavage events, mainly at uracil‐guanine and adenine‐adenine pairs. Proteasome inhibition via dsProsβ1 increased mitochondrial and cytoskeletal proteins while reducing translation‐related and mRNA‐binding proteins.
Doga Cedden, Gözde Güney, Michael Rostás, Stefan Scholten   +3 more
wiley   +1 more source

Differential utilization of NF-kappaB RELA and RELB in response to extracellular versus intracellular polyIC stimulation in HT1080 cells

BMC Immunology, 2011
Background Pattern recognition receptors (PRRs) for double-stranded RNA (dsRNA) are components of innate immunity that recognize the presence of viral infection and initiate efficient defense mechanisms.
Tsao Ming-Sound, Yun James J, Der Sandy D   +2 more
doaj   +1 more source

Synthetic polymer nanocarrier enhances dsRNA stability but not RNAi efficacy in Brassicogethes aeneus

Pest Management Science, EarlyView.
Experimental setup for dsRNA feeding in Brassicogethes aeneus to evaluate the effect of a synthetic polymer nanocarrier on RNAi efficiency. The experiment assessed whether the nanocarrier enhances RNAi after oral dsRNA delivery. (a) Stability of dsRNA–nanocarrier complexes was tested in simulated gut conditions. (b) Gene expression of αCOP and dre4 was
Triin Kallavus, Liina Soonvald, Iris Callewaert, Ana Isabel Silva, Ericmar Ávila Dos Santos, Silva Vilumets, Riina Kaasik, Sven Vereecken, Lenny Van Daele, Peter Dubruel, Jonathan Willow, Clauvis Nji Tizi Taning, Kristof De Schutter, Eve Veromann   +13 more
wiley   +1 more source

Exogenous dsRNA triggers sequence-specific RNAi and fungal stress responses to control Magnaporthe oryzae in Brachypodium distachyon

Communications Biology
In vertebrates and plants, dsRNA plays crucial roles as PAMP and as a mediator of RNAi. How higher fungi respond to dsRNA is not known. We demonstrate that Magnaporthe oryzae (Mo), a globally significant crop pathogen, internalizes dsRNA across a broad ...
Ying Zheng, Benjamin Moorlach, Desiree Jakobs-Schönwandt, Anant Patel, Chiara Pastacaldi, Stefan Jacob, Ana R. Sede, Manfred Heinlein, Minna M. Poranen, Karl-Heinz Kogel, Maria Ladera Carmona   +10 more
doaj   +1 more source

Utilization of Bacteriophage phi6 for the Production of High-Quality Double-Stranded RNA Molecules

Viruses
Double-stranded RNA (dsRNA) molecules are mediators of RNA interference (RNAi) in eukaryotic cells. RNAi is a conserved mechanism of post-transcriptional silencing of genes cognate to the sequences of the applied dsRNA.
Alesia A. Levanova, Minna M. Poranen
doaj   +1 more source

Chitosan‐protected dsRNA increases efficacy against Helicoverpa armigera without compromising biosafety for pollinators

Pest Management Science, EarlyView.
Chitosan/tripolyphosphate/double‐stranded RNA (dsRNA) nanoparticles enhance RNA interference efficacy of orally delivered dsRNA against Helicoverpa armigera. Nanoparticles reduce the LC50 and do not cause deleterious effects or toxicity on beneficial insects.
Daniel DN Vasquez, Lays A Teixeira, Rangel F Alves, Raire Cavalcante, Leonardo LP Macedo, Carmen SS Pires, Luciano P Silva, Maria CM Silva, Feras M Afifi, Carolina V Morgante, Martin G Edwards, Angharad MR Gatehouse, Maria F Grossi‐de‐Sa   +12 more
wiley   +1 more source