Results 101 to 110 of about 56,868 (220)

RNAi‐based functional genomics tools for the beet leafhopper using microinjection and nanoparticle‐based topical spray

Insect Science, EarlyView.
• First demonstration of functional RNAi in the beet leafhopper, Neoaliturus tenellus, using both dsRNA microinjection and star polycation nanoparticle‐mediated topical spray delivery.•Microinjection achieved over 90% knockdown of Gus and Pum3, revealing gene‐specific fitness effects on survival and fecundity in beet leafhopper.•Nanoparticle‐mediated ...
Jinlong Han, Maria Paula Mejia Alonzo, Meihua Cui, Katherine O. Puffer, Anna M. Wolff, Garret M. Miyake, Oliver T. Neher, Vamsi J. Nalam, Punya Nachappa   +8 more
wiley   +1 more source

Transcriptomics Unveil Dsx1 as a Critical Regulator in Sexual Dimorphism of Crustaceans

Integrative Zoology, EarlyView.
Sexually dimorphic traits are involved in reproductive competition and are shaped by sex‐biased gene expression. This study identifies Dsx1 as a key male‐biased gene in Morinoia aosen and demonstrates through RNA interference that its disruption feminizes male‐specific T3 leg structures.
Yan Tong, Wenpei Xin, Zeyu Liu, Yami Zheng, Mengyi Huang, Hongguang Liu, Decai Lu, Wei Peng, Zhonge Hou   +8 more
wiley   +1 more source

Influence of cellular dsRNA binding proteins in the replication process of a dsRNA virus

, 2012
Die Fragestellung dieser Arbeit war, ob Proteine des Virus der infektiösen Bursitis (IBDV) mit den aviären Proteinen p68 und NF45 (nuclear factor 45) interagieren. Beide Hühnerproteine wurden exprimiert und gereinigt um spezifische Seren herzustellen.
openaire   +2 more sources

Gasdermin‐Mediated Pyroptosis: Novel Strategies Against Colorectal Cancer

Cancer Science, EarlyView.
Pyroptosis is a double‐edged sword in colorectal cancer (CRC): chronic low‐grade pyroptosis promotes tumor progression, whereas acute induction of pyroptosis in cancer cells triggers antitumor immunity and enhances therapeutic response. Targeting gasdermin‐mediated pyroptosis represents a promising strategy for CRC treatment.
Kaibo Guo, Yuqian Feng, Jiamin Lu, Nuerbiye Abudurexiti, Xinlei Zhou, Mengfan Wei, Song Zheng   +6 more
wiley   +1 more source

Potencial tehnologije SIGS za zatiranje glive Verticillium nonalfalfae

Hmeljarski Bilten
Gliva Verticillium nonalfalfae je talni patogen hmelja (Humulus lupulus L.), ki povzroča bolezen verticilijsko uvelost, ki lahko ob pojavu visoko virulentnih izolatov v hmeljiščih popolnoma uniči pridelek.
Taja Jeseničnik
doaj  

Monocytes Derived From Human Pluripotent Stem Cells Engineered for Detection of Pyrogens

Cell Proliferation, EarlyView.
In summary, we have developed a monocyte‐based reporter system using hESC‐Mono for interrogating the NF‐κB signalling axis, a central pathway governing pyrogenic responses. Our platform enables rapid and dose‐dependent detection of both endotoxins (LPS) and non‐endotoxins (LTA and Zymosan).
Jingjing Liu, Bingyu Cai, Tingting Gao, Wumei Wei, Liu Wang, Qi Zhou, Wei Li, Jie Hao, Jun Wu   +8 more
wiley   +1 more source

Double-stranded RNA antiviral signature in early multiple sclerosis

Frontiers in Microbiology
IntroductionViruses, particularly Epstein–Barr virus (EBV), are strongly implicated in multiple sclerosis (MS) pathogenesis, yet reliable biomarkers of active viral replication remain limited.
Vasileios Gouzouasis, Vasileios Gouzouasis, Ioannis Sarrigeorgiou, Margaritis Tsifintaris, Nikos Markoglou, Urania Georgopoulou, Peggy Lymberi, Maria Anagnostouli, Lesley Probert, Antonis Giannakakis, Antonis Giannakakis   +10 more
doaj   +1 more source

The E‐cadherin‐Wnt‐mir‐994 Axis Repurposes a Cadherin Switch for Niche Robustness and Germline Stem Cell Maintenance

Cell Proliferation, EarlyView.
In the Drosophila ovarian niche, an E‐cadherin‐to‐N‐cadherin switch, mediated by Wnt‐mir‐994 signalling, is repurposed to ensure niche resilience. This compensatory mechanism maintains niche integrity and stem cell support upon E‐cadherin loss, revealing a robustness circuit.
Renjun Tu, Hiu Laam Yau, Runzhi Deng, Sarah E. Webb, Shiyuan Chen, Jianquan Ni, Ting Xie   +6 more
wiley   +1 more source

Malaria mosquito antimicrobial defence requires immunity and detoxification gene regulation by Lola

Insect Molecular Biology, EarlyView.
Malaria mosquito antimicrobial defence requires upregulation of lola. Attenuation of lola in the midgut of Anopheles albimanus mosquitoes inhibits the upregulation of immunity genes induced by challenge. Putative target genes of the Lola transcription factor were revealed by lola attenuation, including Cecropin‐C, Draper, PPAF2, Clip‐domain serine ...
Heidi Espadas‐Álvarez, Verónica Valverde‐Garduño   +1 more
wiley   +1 more source

Release of mitochondrial dsRNA into the cytosol is a key driver of the inflammatory phenotype of senescent cells

Nature Communications
The escape of mitochondrial double-stranded dsRNA (mt-dsRNA) into the cytosol has been recently linked to a number of inflammatory diseases. Here, we report that the release of mt-dsRNA into the cytosol is a general feature of senescent cells and a ...
Vanessa López-Polo, Mate Maus, Emmanouil Zacharioudakis, Miguel Lafarga, Camille Stephan-Otto Attolini, Francisco D. M. Marques, Marta Kovatcheva, Evripidis Gavathiotis, Manuel Serrano   +8 more
doaj   +1 more source