Results 111 to 120 of about 73,913 (251)
dsRNA uptake in adult Drosophila
, 2011 International audienceRNA interference (RNAi) is a conserved sequence-specific gene silencing mechanism that is induced by double-stranded RNA (dsRNA).
Saleh, Maria-Carla, P +3 more
core +1 more source
Calcitonin‐like diuretic hormone influences reproduction in adult female Rhodnius prolixus
Insect Science, EarlyView.Highlights • CT/DH‐like immunoreactivity is present in female reproductive tissues • CT/DH receptor transcripts are expressed in the fat body and ovaries • RNAi knockdown of CT/DH receptors reduces egg number and hatching rate • Rhopr‐CT/DH increases RhoprVg1 and RhoprVgR expression in ovaries in vitro • Rhopr‐CT/DH stimulates oviduct contractions in a
Areej N. Al‐Dailami +2 more
wiley +1 more source
DsRNA-mediated knockdown of cactus results in increased Toll signaling.
(a-h) The expression of cactus (a, e) and the antimicrobial peptides defensin-2 (b, f), defensin-3 (c, g) and cecropin-2 (d, h) were assayed via RT-qPCR in whole adult beetles treated with 250 ng of cactus or malE dsRNA and then septically challenged ...
Ann T. Tate (3314862) +3 more
core +1 more source
Generation of transgenic tobacco plants producing HaEcR dsRNA and GFP dsRNA.
, 2013 (A) pBI121-dsEcR: the constructed pBI121 vector expressing hairpin HaEcR dsRNA in transgenic tobacco plants. See details in materials and methods. (B) Transgenic tobacco plants expressing dsRNAs were obtained using the standard procedure.
Zhao-Jun Wei (320037) +8 more
core +1 more source
LsFAMeT and LsJHAMT coordinate JH biosynthesis to regulate fecundity in Laodelphax striatellus
Insect Science, EarlyView.The small brown planthopper (SBPH), Laodelphax striatellus, can trigger large‐scale outbreaks when encountering suitable habitats during migration due to their strong reproductive ability. This study investigated the role of juvenile hormone (JH) in regulating SBPH reproduction.
Yan Guo +3 more
wiley +1 more source
Phenylmethimazole Blocks dsRNA-Induced IRF3 Nuclear Translocation and Homodimerization
, 2012 Previous studies revealed that phenylmethimazole (C10) inhibits IRF3 signaling, preventing dsRNA-induction of type 1 interferon gene expression, production, and downstream signaling.
Kelly D. McCall +4 more
core +1 more source
TcALS knockdown accelerates metamorphosis via insulin signaling in Tribolium castaneum
Insect Science, EarlyView.RNAi silencing of TcALS accelerates larva‐to‐pupa metamorphosis in Tribolium castaneum. Abstract Insect metamorphosis is an evolutionary adaptation precisely regulated by nutritional and hormonal cues. The red flour beetle Tribolium castaneum, a major stored‐product pest, serves as an excellent model for studying this process.
Min Li +8 more
wiley +1 more source
Journal of VirologyDouble-stranded RNA (dsRNA), which induces an innate immune response against viral infections, is rarely detected in influenza A virus (IAV)-infected cells.
Masahiro Nakano +7 more
doaj +1 more source
Insect Science, EarlyView.• First demonstration of functional RNAi in the beet leafhopper, Neoaliturus tenellus, using both dsRNA microinjection and star polycation nanoparticle‐mediated topical spray delivery.•Microinjection achieved over 90% knockdown of Gus and Pum3, revealing gene‐specific fitness effects on survival and fecundity in beet leafhopper.•Nanoparticle‐mediated ...
Jinlong Han +8 more
wiley +1 more source
Journal of Biological EngineeringBackground Infections with various RNA viruses and certain DNA viruses may produce double-stranded RNA (dsRNA) during replication, which trigger host innate immune responses.
Michihito Sasaki +8 more
doaj +1 more source