Results 111 to 120 of about 68,502 (270)

Protective Effects of Long Double-Stranded RNA with Different CpG Motifs against Miamiensis avidus and Viral Hemorrhagic Septicemia Virus (VHSV) Infections in Olive Flounder (Paralichthys olivaceus)

Fishes
The South Korean aquaculture industry has incurred considerable production losses due to various infectious diseases. Artificially synthesized polyinosinic–polycytidylic acid (poly I:C), structurally similar to double-stranded RNA (dsRNA) and cytidine ...
Hee-Jae Choi, Da-Yeon Choi, Jung-Jin Park, Hye Jin Jung, Bo-Seong Kim, Sung-Chul Hong, Jun-Hwan Kim, Yue Jai Kang   +7 more
doaj   +1 more source

Primer sequences for dsRNA and qRT-PCR.

, 2016
dsRNA: ADAM10 dsRNA for knock-down primer. qRT-PCR: mRNA expression check primer. (DOCX)
Inchul Choi (417288), Jeongwoo Kwon (702556), Sung-min Jeong (2589943), Nam-Hyung Kim (293371)   +3 more
core   +1 more source

HvarAKR1B1‐mediated tolerance in Hippodamia variegata: Deciphering the metabolic adaptation and fitness costs under prolonged starvation

Pest Management Science, EarlyView.
Starvation stress significantly affected the growth and development of Hippodamia variegata. Combined transcriptome and metabolome analysis and RNA interference verification revealed that HvarAKR1B1 may be involved in the response of Hippodamia variegata to starvation stress.
Bing‐mei Song, De‐qin Hu, Yuan Li, Wang‐quan Jiao, Xiao‐li Ma, Hong‐sheng Pan   +5 more
wiley   +1 more source

Intracellular delivery of synthetic dsRNA to leukemic cells induces apoptotic and necrotic cell death

, 2016
The type of tumour cell death dictates the type of adaptive immune response mounted against the tumours. In haematological malignancies such as acute myeloid leukaemia (AML), immune evasion due to the poor immunogenicity of leukemic cells is a major ...
Mahmud, SM, Idris, A, Mek, KJ
core  

The effect of dsRNA competition on RNAi knockdown efficiency.

, 2012
(A) Competitor dsRNA injected alone. (1) DsRed dsRNA. (2) Ubx dsRNA. (B) Competitor and reporter dsRNA co-injected at a 10 to 1 ratio. (1) DsRed dsRNA injected at 1 µg/µL (competitor). EGFP dsRNA injected at 0.1 µg/µL (reporter).
Yoshinori Tomoyasu (48231), Keita Miyata (124456), Susan J. Brown (19392), Sherry C. Miller (124453)   +3 more
core   +1 more source

Multimodal targets of (‐)‐α‐thujone mediating avoidance behavior of a stored‐products pest, Tribolium castaneum, in response to irritant stimuli

Pest Management Science, EarlyView.
We identified four kinds of target molecules against (‐)‐α‐thujone associating avoidance behavior in Tribolium castaneum, indicating multimodality. Abstract BACKGROUND The reciprocal evolutionary processes among phytophagous insects and host plants involve host shift in the herbivorous insects in response to defense chemicals, such as plant‐derived ...
Kenji Shimomura, Yuto Suzuki, Soshi Ino, Takehito Terajima, Motohiro Tomizawa   +4 more
wiley   +1 more source

Exogenous Application of IR-Specific dsRNA Inhibits Infection of Cucumber Green Mottle Mosaic Virus in Watermelon

Agronomy
Cucumber green mottle mosaic virus (CGMMV) represents a serious threat in the production of watermelon. Small RNAs facilitate a mechanism known as RNA interference (RNAi), which regulates gene expression.
Yanhui Wang, Liming Liu, Yongqiang Fan, Yanli Han, Zhiling Liang, Yanfei Geng, Fengnan Liu, Qinsheng Gu, Baoshan Kang, Chaoxi Luo   +9 more
doaj   +1 more source

Relative ClCPR mRNA levels in control (malE dsRNA) and ClCPR dsRNA injected bed bugs.

, 2012
(A) The ClCPR mRNA levels were quantified by qRT-PCR at 5 days after dsRNA injection in control (malE dsRNA) and ClCPR dsRNA treated bed bugs with different doses of dsRNA.
Michael F. Potter (185572), Fang Zhu (185566), Sarah Sams (185567), Subba R. Palli (185574), Kenneth F. Haynes (185570), Tim Moural (185568)   +5 more
core   +1 more source

Identification of broadly expressed nervous system‐related genes as effective RNAi targets in the brown marmorated stink bug, Halyomorpha halys

Pest Management Science, EarlyView.
Broadly expressed genes outperformed brain‐enriched neural targets as RNAi candidates in Halyomorpha halys. Microinjection of dsRNA targeting alpha‐soluble NSF attachment protein (Asnap) or Ras opposite protein (Rop) caused strong feeding suppression and near‐complete mortality, identifying them as promising targets for future oral or spray‐based RNAi ...
Nirakar Panda, Aline Koch, Venkata Partha Sarathi Amineni   +2 more
wiley   +1 more source

PACT prevents aberrant activation of PKR by endogenous dsRNA without sequestration

Nature Communications
The innate immune sensor PKR for double-stranded RNA (dsRNA) is critical for antiviral defense, but its aberrant activation by cellular dsRNA is linked to various diseases.
Sadeem Ahmad, Tao Zou, Jihee Hwang, Linlin Zhao, Xi Wang, Anton Davydenko, Ilana Buchumenski, Patrick Zhuang, Alyssa R. Fishbein, Diego Capcha-Rodriguez, Aaron Orgel, Erez Y. Levanon, Sua Myong, James Chou, Matthew Meyerson, Sun Hur   +15 more
doaj   +1 more source