Results 91 to 100 of about 56,868 (220)

3D‐Printed Milli‐Fluidic Mixing‐Extrusion Platform for Continuous, High‐Throughput LNP Production

Small, EarlyView.
We report a continuous high‐throughput production of lipid nanoparticles (LNPs) by integrating a reusable 3D‐printed milli‐fluidic split‐and‐recombine mixer with inline membrane extrusion. The 3D architecture enables rapid early‐stage mixing, reducing clogging and fouling, while inline extrusion further refines the as‐formed LNPs, improving uniformity,
Leekang Jeon, Seeun Kim, Hanjin Seo, Hyun Su Lee, Gi‐Su Na, Gi‐Ra Yi, Hyomin Lee   +6 more
wiley   +1 more source

Silencing of juvenile hormone‐related genes through RNA interference leads to molt failure and high mortality in the spongy moth

Insect Science, EarlyView.
The feasibility of using RNA interference to control the globally important quarantine pest, the spongy moth. Targeting genes related to JHs play an important role in the growth and development of insects. First, the open reading frames (ORFs) of Ldjhamt and Ldjheh were identified and characterized, and the target genes were cloned and double‐stranded ...
Wenzhuai Ji, Qing Xie, Xiaoxiao Chang, Juan Shi   +3 more
wiley   +1 more source

Universal and quantitative detection of double-stranded RNAs as a signature of pan-virus infections using a luciferase-based biosensor

Journal of Biological Engineering
Background Infections with various RNA viruses and certain DNA viruses may produce double-stranded RNA (dsRNA) during replication, which trigger host innate immune responses.
Michihito Sasaki, Eri Fujii, Satoko Sasaki, Takuma Ariizumi, Kei Konishi, Akihiko Sato, William W. Hall, Hirofumi Sawa, Yasuko Orba   +8 more
doaj   +1 more source

Influenza A virus circumvents the innate immune response through the sequestration of double-stranded RNA

Journal of Virology
Double-stranded RNA (dsRNA), which induces an innate immune response against viral infections, is rarely detected in influenza A virus (IAV)-infected cells.
Masahiro Nakano, Sho Miyamoto, Chiho Ohnishi, Chiharu Nogami, Nanami Hirose, Yoko Fujita-Fujiharu, Yukiko Muramoto, Takeshi Noda   +7 more
doaj   +1 more source

Iflaviruses in arthropods: when small is mighty

Insect Science, EarlyView.
Many arthropod species harbor iflaviruses, which often cause covert (asymptomatic) infections, but may still affect host fitness. We review the impact of iflaviruses on arthropod fitness, immunity, behaviour as well as the iflavirus’ host range, transmission, tissue tropism and the interactions with other microorganisms within arthropods.
Annamaria Mattia, Hannah‐Isadora Huditz, Monique M. van Oers, Vera I.D. Ros   +3 more
wiley   +1 more source

Calcitonin‐like diuretic hormone influences reproduction in adult female Rhodnius prolixus

Insect Science, EarlyView.
Highlights • CT/DH‐like immunoreactivity is present in female reproductive tissues • CT/DH receptor transcripts are expressed in the fat body and ovaries • RNAi knockdown of CT/DH receptors reduces egg number and hatching rate • Rhopr‐CT/DH increases RhoprVg1 and RhoprVgR expression in ovaries in vitro • Rhopr‐CT/DH stimulates oviduct contractions in a
Areej N. Al‐Dailami, Ian Orchard, Angela B. Lange   +2 more
wiley   +1 more source

LsFAMeT and LsJHAMT coordinate JH biosynthesis to regulate fecundity in Laodelphax striatellus

Insect Science, EarlyView.
The small brown planthopper (SBPH), Laodelphax striatellus, can trigger large‐scale outbreaks when encountering suitable habitats during migration due to their strong reproductive ability. This study investigated the role of juvenile hormone (JH) in regulating SBPH reproduction.
Yan Guo, Bing‐Bing Shi, Xue‐Qi Zhang, Qiong Yang   +3 more
wiley   +1 more source

TcALS knockdown accelerates metamorphosis via insulin signaling in Tribolium castaneum

Insect Science, EarlyView.
RNAi silencing of TcALS accelerates larva‐to‐pupa metamorphosis in Tribolium castaneum. Abstract Insect metamorphosis is an evolutionary adaptation precisely regulated by nutritional and hormonal cues. The red flour beetle Tribolium castaneum, a major stored‐product pest, serves as an excellent model for studying this process.
Min Li, Jie Chen, Zi‐Ye Wang, Fei‐Feng Wang, Lu‐Lu Dong, Jun Pan, Min‐Er Li, Lin Yu, Wen Sang   +8 more
wiley   +1 more source

Mathematical modelling of OAS2 activation by dsRNA and effects of dsRNA lengths

, 2020
The activation of 2'-5'-oligoadenylate synthetase (OAS) enzymes by direct interaction with viral double-stranded RNA (dsRNA) is a key part of the innate immune response to viral infection. The downstream effect of the OAS-dsRNA interaction is to degrade the viral single-stranded RNA (ssRNA) to prevent the spread of the virus.
openaire   +1 more source

Assessing various administration strategies for dsRNA vaccine delivery: a concise review of VP15-WSSV research progress in tiger shrimp Penaeus monodon

Jurnal Ilmiah Perikanan dan Kelautan
Graphical Abstract     Highlight Research 1. The dsRNA application should correspond to the developmental stages of shrimp 2. Immersion method was commonly used for larvae for handling many individuals 3.
Andi Parenrengi, Samuel Lante, Emma Suryati, Rosmiati Rosmiati, Sulaeman Sulaeman, Herlinah Herlinah, Yosie Andriani   +6 more
doaj   +1 more source