Results 141 to 150 of about 14,786,969 (255)

Spatial heterogeneity of hotspot mutations and clonal relatedness in ovarian endometrioid carcinoma

Molecular Oncology, EarlyView.
Spatially resolved analysis revealed heterogeneous distributions of selected cancer‐associated mutations within ovarian endometrioid carcinomas. Shared mutations between paired endometrial and ovarian tumors supported clonal relatedness. These findings highlight the value of sampling multiple tumor regions when interpreting molecular profiles ...
Takahito Ashihara, Kosuke Murakami, Shiro Takamatsu, Akiko Kanto, Seiichi Mori, Tomoyuki Otani, Kazuko Sakai, Yasushi Kotani, Kazuto Nishio, Kaoru Abiko, Noriomi Matsumura   +10 more
wiley   +1 more source

Multiple merger coalescent inference of effective population size. [PDF]

Philos Trans R Soc Lond B Biol Sci
Zhang J, Palacios JA.
europepmc   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Dealing With the Complexity of Effective Population Size in Conservation Practice. [PDF]

Evol Appl
Fedorca A, Mergeay J, Akinyele AO, Albayrak T, Biebach I, Brambilla A, Burger PA, Buzan E, Curik I, Gargiulo R, Godoy JA, González-Martínez SC, Grossen C, Heuertz M, Hoban S, Howard-McCombe J, Kachamakova M, Klinga P, Köppä V, Neugebauer E, Paz-Vinas I, Pearman PB, Pérez-Sorribes L, Rinkevich B, Russo IM, Theraroz A, Thomas NE, Westergren M, Winter S, Laikre L, Kopatz A.   +30 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Estimating the Effective Population Size Across Space and Time in the Critically Endangered Western Chimpanzee in Guinea-Bissau: Challenges and Implications for Conservation Management. [PDF]

Evol Appl
da Silva MJF, Borges F, Gerini F, Sá RM, Silva F, Maié T, Hernández-Alonso G, Ramos-Madrigal J, Gopalakrishnan S, Aleixo-Pais I, Djaló SL, Fernandes N, Camará I, Regalla A, Casanova C, Costa M, Colmonero-Costeira I, Rodríguez Fernandes C, Chikhi L, Minhós T, Bruford MW.   +20 more
europepmc   +1 more source

Effective population size in a partially clonal plant is not predicted by the number of genetic individuals. [PDF]

Evol Appl, 2023
Gargiulo R, Waples RS, Grow AK, Shefferson RP, Viruel J, Fay MF, Kull T.   +6 more
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Spatio-Temporal Changes in Effective Population Size in an Expanding Metapopulation of Eurasian Otters. [PDF]

Evol Appl
Thomas NE, Chadwick EA, Bruford MW, Hailer F.   +3 more
europepmc   +1 more source

Identity-by-descent-based estimation of the X chromosome effective population size with application to sex-specific demographic history. [PDF]

G3 (Bethesda), 2023
Cai R, Browning BL, Browning SR.
europepmc   +1 more source