Results 31 to 40 of about 3,027 (160)

Elastin-like Polypeptides in Development of Nanomaterials for Application in the Medical Field

Frontiers in Nanotechnology, 2022
Elastin-like polypeptides (ELPs) are biopolymers formed by amino acid sequences derived from tropoelastin. These biomolecules can be soluble below critical temperatures, forming aggregates at higher temperatures, which makes them an interesting source ...
Letícia Ferreira Lima, Letícia Ferreira Lima, Mauricio Gonçalves Da Costa Sousa, Gisele Regina Rodrigues, Gisele Regina Rodrigues, Gisele Regina Rodrigues, Kamila Botelho Sampaio de Oliveira, Kamila Botelho Sampaio de Oliveira, Ana Margarida Pereira, Ana Margarida Pereira, André da Costa, André da Costa, Raul Machado, Raul Machado, Octavio Luiz Franco, Octavio Luiz Franco, Octavio Luiz Franco, Simoni Campos Dias, Simoni Campos Dias, Simoni Campos Dias   +19 more
doaj   +1 more source

Elastin-Like Polypeptide Based Hydroxyapatite Bionanocomposites [PDF]

Biomacromolecules, 2011
In nature, organic matrix macromolecules play a critical role in enhancing the mechanical properties of biomineralized composites such as bone and teeth. Designing artificial matrix analogues is promising but challenging because relatively little is known about how natural matrix components function.
Eddie, Wang, Sang-Hyuk, Lee, Seung-Wuk, Lee   +2 more
openaire   +2 more sources

Composite of Elastin-Based Matrix and Electrospun Poly(L-Lactic Acid) Fibers: A Potential Smart Drug Delivery System

Frontiers in Bioengineering and Biotechnology, 2018
Stimuli-responsive hydrogel matrices are inspiring manifold applications in controlled delivery of bioactive compounds. Elastin-derived polypeptides form hydrogel matrices that may release bioactive moieties as a function of local increase of active ...
Antonella Bandiera, Sabina Passamonti, Luisa Stella Dolci, Maria Letizia Focarete, Maria Letizia Focarete   +4 more
doaj   +1 more source

High-yield recombinant expression and purification of marginally soluble, short elastin-like polypeptides

BioTechniques, 2016
The protocol described here is designed as an extension of existing techniques for creating elastin-like polypeptides. It allows for the expression and purification of elastin-like polypeptide (ELP) constructs that are poorly expressed or have very low ...
Markian S. Bahniuk, Abdullah K. Alshememry, Larry D. Unsworth   +2 more
doaj   +1 more source

Fusions of Elastin-Like Polypeptides to Pharmaceutical Proteins [PDF]

, 2012
Elastin-like polypeptides (ELPs) are a class of stimulus-responsive biopolymers whose physicochemical properties and biocompatibility are particularly suitable for in vivo applications, such as drug delivery and tissue engineering. The lower critical solution temperature (LCST) behavior of ELPs allows them to be utilized as soluble macromolecules below
Wafa, Hassouneh, Sarah R, MacEwan, Ashutosh, Chilkoti   +2 more
openaire   +2 more sources

Biophysical Characterization of an Elastin-Like-Polypeptide [PDF]

Biophysical Journal, 2011
Perhaps the largest impediment in cancer therapies has been the inability to effectively target systemically delivered therapeutics to cancerous cells. A recent and novel approach to this problem has been to chemically conjugate the therapeutic to an Elastin-Like-Polypeptide (ELP).
Lyons, Daniel, Bidwell, Gene L., Kramer, Wolfgang H., Raucher, Drazen, Correia, John J.   +4 more
openaire   +1 more source

Tuning the Properties of Protein-Based Polymers Using High-Performance Orthogonal Translation Systems for the Incorporation of Aromatic Non-Canonical Amino Acids

Frontiers in Bioengineering and Biotechnology, 2022
The incorporation of non-canonical amino acids (ncAAs) using engineered aminoacyl-tRNA synthetases (aaRSs) has emerged as a powerful methodology to expand the chemical repertoire of proteins.
Osher Gueta, Ortal Sheinenzon, Rotem Azulay, Hadas Shalit, Daniela S. Strugach, Dagan Hadar, Sigal Gelkop, Anat Milo, Miriam Amiram   +8 more
doaj   +1 more source

Polymorphisms in the human tropoelastin gene modify in vitro self-assembly and mechanical properties of elastin-like polypeptides. [PDF]

PLoS ONE, 2012
Elastin is a major structural component of elastic fibres that provide properties of stretch and recoil to tissues such as arteries, lung and skin. Remarkably, after initial deposition of elastin there is normally no subsequent turnover of this protein ...
David He, Ming Miao, Eva E Sitarz, Lisa D Muiznieks, Sean Reichheld, Richard J Stahl, Fred W Keeley, John Parkinson   +7 more
doaj   +1 more source

pH-Responsive Elastin-Like Polypeptide Designer Condensates

ACS Applied Materials & Interfaces, 2023
Biomolecular condensates are macromolecular complexes formed by liquid-liquid phase separation. They regulate key biological functions by reversibly compartmentalizing molecules in cells, in a stimulus-dependent manner. Designing stimuli-responsive synthetic condensates is crucial for engineering compartmentalized synthetic cells that are able to mimic
Robbert J. de Haas, Ketan A. Ganar, Siddharth Deshpande, Renko de Vries   +3 more
openaire   +2 more sources

Temperature-dependent morphology of hybrid nanoflowers from elastin-like polypeptides

APL Materials, 2014
We report a method for creating hybrid organic-inorganic “nanoflowers” using calcium or copper ions as the inorganic component and a recombinantly expressed elastin-like polypeptide (ELP) as the organic component.
Koushik Ghosh, Eva Rose M. Balog, Prakash Sista, Darrick J. Williams, Daniel Kelly, Jennifer S. Martinez, Reginaldo C. Rocha   +6 more
doaj   +1 more source