A Novel Protein Purification Approach Using Elastin-Like Polypeptides (ELP) With His-Tag Assistance [PDF]
Bio-ProtocolProtein purification is essential for drug development, antibody production, and structural biology. We propose a cost-effective chromatography method using elastin-like polypeptide (ELP) as an aggregating core.
Young Chae, Han Shin
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Thermally-Responsive Loading and Release of Elastin-Like Polypeptides from Contact Lenses [PDF]
Pharmaceutics, 2019 Contact lenses are widely prescribed for vision correction, and as such they are an attractive platform for drug delivery to the anterior segment of the eye.
Wan Wang +4 more
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Evaluation of Elastin-Like Polypeptides for Tumor Targeted Delivery of Doxorubicin to Glioblastoma [PDF]
Molecules, 2019 To increase treatment efficiency for glioblastoma, we have developed a system to selectively deliver chemotherapeutic doxorubicin (Dox) to Glioblastoma (GBM) tumors.
Sonja Dragojevic +2 more
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Biomimetic Elastin-Like Polypeptides as Materials for the Activation of Mechanophoric Catalysts
Organic Materials, 2020 Elastin-like polypeptides (ELPs) are well known for their elastic and thermoresponsive behaviors. Their elasticity originates from the formation of a β-spiral which is the consequence of stacking type-II β-turns, formed from individual VPGVG pentapeptide
Sebastian Funtan +3 more
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Applications of elastin-like polypeptides in tissue engineering [PDF]
Advanced Drug Delivery Reviews, 2010 Elastin-like polypeptides (ELPs) have found utility in tissue engineering applications, not only because they are biocompatible, biodegradable, and non-immunogenic, but also because their amino acid sequence and molecular weight can be precisely controlled at the genetic or synthetic level, affording exquisite control over final protein functionality ...
Lori Setton +2 more
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Intracellular Delivery of Rapamycin From FKBP Elastin-Like Polypeptides Is Consistent With Macropinocytosis [PDF]
Frontiers in Pharmacology, 2018 Rapamycin (Rapa) is a highly potent drug; however, its clinical potential is limited by poor solubility, bioavailability, and cytotoxicity. To improve Rapa delivery, our team has fused the cognate protein receptor for Rapa, FKBP12, to high molecular ...
Santosh Peddi +4 more
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Generation of a Monoclonal Antibody to Detect Elastin-like Polypeptides. [PDF]
Biomacromolecules, 2019 The identification and use of antibodies dominate the biologic, clinical diagnostic, and therapeutic landscapes. In particular, antibodies have become essential tools in a variety of protein analytical experiments and to study the disposition of biologic therapeutics.
Kouhi A +6 more
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Isolation of nucleic acids using liquid–liquid phase separation of pH-sensitive elastin-like polypeptides [PDF]
Scientific ReportsExtraction of nucleic acids (NAs) is critical for many methods in molecular biology and bioanalytical chemistry. NA extraction has been extensively studied and optimized for a wide range of applications and its importance to society has significantly ...
Telmo Díez Pérez +6 more
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Elastin-like polypeptides as models of intrinsically disordered proteins. [PDF]
FEBS Lett, 2015 Elastin‐like polypeptides (ELPs) are a class of stimuli‐responsive biopolymers inspired by the intrinsically disordered domains of tropoelastin that are composed of repeats of the VPGXG pentapeptide motif, where X is a “guest residue”. They undergo a reversible, thermally triggered lower critical solution temperature (LCST) phase transition, which has ...
Roberts S, Dzuricky M, Chilkoti A.
europepmc +4 more sources
Self-assembly/disassembly hysteresis of nanoparticles composed of marginally soluble, short elastin-like polypeptides [PDF]
Journal of Nanobiotechnology, 2018 Background Elastin-like polypeptides (ELPs) are a fascinating biomaterial that has undergone copious development for a variety of therapeutic applications including as a nanoscale drug delivery vehicle.
Markian S. Bahniuk +3 more
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