Results 41 to 50 of about 3,027 (160)

Engineered bacterial host for genetic encoding of physiologically stable protein nitration

Frontiers in Molecular Biosciences, 2022
Across scales, many biological phenomena, such as protein folding or bioadhesion and cohesion, rely on synergistic effects of different amino acid side chains at multiple positions in the protein sequence. These are often fine-tuned by post-translational
Nikolaj G. Koch, Nikolaj G. Koch, Tobias Baumann, Jessica H. Nickling, Anna Dziegielewski, Nediljko Budisa, Nediljko Budisa   +6 more
doaj   +1 more source

The application of elastin-like peptides in cancer, tissue engineering and ocular disease

OpenNano, 2023
Elastin-like polypeptides (ELPs) are macromolecular biopolymers composed of natural amino acids. ELPs can be prepared by genetic engineering so that their amino acid sequences and molecular weight can be precisely manipulated.
Dabo Jiang, Yang Yang, Xiuyi Yang, Boxuan Wang, Wenxuan Fan, Yuchen Liu, Xiaofei Xin, Lifang Yin   +7 more
doaj   +1 more source

Intracellular construction of topology-controlled polypeptide nanostructures with diverse biological functions

Nature Communications, 2017
The intracellular topology of a nanostructure plays a major role in its interactions with the cell and accordingly, its biological applications. Here, the authors design peptides that intracellularly polymerize into elastin-like polypeptides and assemble
Li-Li Li, Sheng-Lin Qiao, Wei-Jiao Liu, Yang Ma, Dong Wan, Jie Pan, Hao Wang   +6 more
doaj   +1 more source

Secreted production of an elastin-like polypeptide by Pichia pastoris [PDF]

Applied Microbiology and Biotechnology, 2009
Elastin-like polypeptides (ELPs) are biocompatible designer polypeptides with inverse temperature transition behavior in solution. They have a wide variety of possible applications and a potential medical importance. Currently, production of ELPs is done at lab scale in Escherichia coli shake flask cultures. With a view to future large scale production,
Schipperus, R., Teeuwen, R.L.M., Werten, M.W.T., Eggink, G., de Wolf, F.A.   +4 more
openaire   +3 more sources

Induction of protein body formation in plant leaves by elastin-like polypeptide fusions

BMC Biology, 2009
Background Elastin-like polypeptides are synthetic biopolymers composed of a repeating pentapeptide 'VPGXG' sequence that are valuable for the simple non-chromatographic purification of recombinant proteins.
Joensuu Jussi J, Conley Andrew J, Menassa Rima, Brandle Jim E   +3 more
doaj   +1 more source

Aggregation-Dispersion Chromatography: Application of Elastin-like Polypeptides

Separations
Protein purification is a crucial step for various downstream applications like drug development, antibody preparation, and structure determination. The constant pursuit is for methods that are more efficient and cost-effective.
Han Bin Shin, Young Kee Chae
doaj   +1 more source

Effect of detergents on the thermal behavior of elastin‐like polypeptides [PDF]

Biopolymers, 2012
AbstractElastin‐like polypeptide (ELP) fusions have been designed to allow large‐scale, nonchromatographic purification of many soluble proteins by using the inverse transition cycling (ITC) method; however, the sensitivity of the aqueous lower critical solubility phase transition temperature (Tt) of ELPs to the addition of cosolutes, including ...
Arjun, Thapa, Wei, Han, Robin H, Simons, Ashutosh, Chilkoti, Eva Y, Chi, Gabriel P, López   +5 more
openaire   +2 more sources

Cell Adhesion by Design: Engineering Tissue Culture Scaffolds With Adhesion Cues

Advanced Materials Interfaces, EarlyView.
ABSTRACT In scaffold‐based tissue engineering, the matrix should provide adequate adhesion cues for cell attachment, spreading, and function. Given the multitude of adhesion receptors and the diversity of scaffolds, there are many approaches to render scaffolds adhesive, even though they are not all equivalent.
Dalia Dranseike, Dhananjay V. Deshmukh, Mark W. Tibbitt, Céline Labouesse   +3 more
wiley   +1 more source

Critical factors for the bulk adhesion of engineered elastomeric proteins [PDF]

Royal Society Open Science, 2018
Many protein-based materials, such as soy and mussel adhesive proteins, have been the subject of scientific and commercial interest. Recently, a variety of protein adhesives have been isolated from diverse sources such as insects, frogs and squid ring ...
M. Jane Brennan, Sydney E. Hollingshead, Jonathan J. Wilker, Julie C. Liu   +3 more
doaj   +1 more source

Neurovascular coupling in bone regeneration: Mechanisms, advanced biomaterials and challenges

BMEMat, EarlyView.
This figure illustrates various material strategies for neurovascularized bone regeneration, including electroactive scaffolds, ion‐loaded materials, drug delivery systems, surface modifications, cells/cell products, growth factors, and peptides. These approaches aim to synergistically promote the regeneration of neural, vascular, and bone tissues ...
Yixin Ma, Nan Jiang, Liyuan Chen, He Zhang, Xinjia Cai, Karan Gulati, Danqing He, Zhigang Cai, Yan Liu   +8 more
wiley   +1 more source