Results 41 to 50 of about 3,029 (158)

Polymorphisms in the human tropoelastin gene modify in vitro self-assembly and mechanical properties of elastin-like polypeptides. [PDF]

PLoS ONE, 2012
Elastin is a major structural component of elastic fibres that provide properties of stretch and recoil to tissues such as arteries, lung and skin. Remarkably, after initial deposition of elastin there is normally no subsequent turnover of this protein ...
David He, Ming Miao, Eva E Sitarz, Lisa D Muiznieks, Sean Reichheld, Richard J Stahl, Fred W Keeley, John Parkinson   +7 more
doaj   +1 more source

Temperature-dependent morphology of hybrid nanoflowers from elastin-like polypeptides

APL Materials, 2014
We report a method for creating hybrid organic-inorganic “nanoflowers” using calcium or copper ions as the inorganic component and a recombinantly expressed elastin-like polypeptide (ELP) as the organic component.
Koushik Ghosh, Eva Rose M. Balog, Prakash Sista, Darrick J. Williams, Daniel Kelly, Jennifer S. Martinez, Reginaldo C. Rocha   +6 more
doaj   +1 more source

Engineered bacterial host for genetic encoding of physiologically stable protein nitration

Frontiers in Molecular Biosciences, 2022
Across scales, many biological phenomena, such as protein folding or bioadhesion and cohesion, rely on synergistic effects of different amino acid side chains at multiple positions in the protein sequence. These are often fine-tuned by post-translational
Nikolaj G. Koch, Nikolaj G. Koch, Tobias Baumann, Jessica H. Nickling, Anna Dziegielewski, Nediljko Budisa, Nediljko Budisa   +6 more
doaj   +1 more source

The application of elastin-like peptides in cancer, tissue engineering and ocular disease

OpenNano, 2023
Elastin-like polypeptides (ELPs) are macromolecular biopolymers composed of natural amino acids. ELPs can be prepared by genetic engineering so that their amino acid sequences and molecular weight can be precisely manipulated.
Dabo Jiang, Yang Yang, Xiuyi Yang, Boxuan Wang, Wenxuan Fan, Yuchen Liu, Xiaofei Xin, Lifang Yin   +7 more
doaj   +1 more source

Intracellular construction of topology-controlled polypeptide nanostructures with diverse biological functions

Nature Communications, 2017
The intracellular topology of a nanostructure plays a major role in its interactions with the cell and accordingly, its biological applications. Here, the authors design peptides that intracellularly polymerize into elastin-like polypeptides and assemble
Li-Li Li, Sheng-Lin Qiao, Wei-Jiao Liu, Yang Ma, Dong Wan, Jie Pan, Hao Wang   +6 more
doaj   +1 more source

Induction of protein body formation in plant leaves by elastin-like polypeptide fusions

BMC Biology, 2009
Background Elastin-like polypeptides are synthetic biopolymers composed of a repeating pentapeptide 'VPGXG' sequence that are valuable for the simple non-chromatographic purification of recombinant proteins.
Joensuu Jussi J, Conley Andrew J, Menassa Rima, Brandle Jim E   +3 more
doaj   +1 more source

Aggregation-Dispersion Chromatography: Application of Elastin-like Polypeptides

Separations
Protein purification is a crucial step for various downstream applications like drug development, antibody preparation, and structure determination. The constant pursuit is for methods that are more efficient and cost-effective.
Han Bin Shin, Young Kee Chae
doaj   +1 more source

pH-Responsive Elastin-Like Polypeptide Designer Condensates

ACS Applied Materials & Interfaces, 2023
Biomolecular condensates are macromolecular complexes formed by liquid-liquid phase separation. They regulate key biological functions by reversibly compartmentalizing molecules in cells, in a stimulus-dependent manner. Designing stimuli-responsive synthetic condensates is crucial for engineering compartmentalized synthetic cells that are able to mimic
Robbert J. de Haas, Ketan A. Ganar, Siddharth Deshpande, Renko de Vries   +3 more
openaire   +2 more sources

Extrusion Bioprinting for Wound Healing: Innovations in Functionalized Bioinks and Bioprinting Technology

Advanced Materials Technologies, EarlyView.
Extrusion‐based bioprinting (EBB) has emerged as a versatile biofabrication platform capable of precisely depositing bioinks composed of biomaterials, cells, and bioactive agents to generate patient‐specific, biomimetic skin constructs. This paper presents a state‐of‐the‐art and forward‐looking overview of EBB for wound healing, encompassing printing ...
Hien‐Phuong Le, Anh Tuan Trong Tran, Ammara Waheed, Tran Thanh Tung, Dusan Losic   +4 more
wiley   +1 more source

Secreted production of an elastin-like polypeptide by Pichia pastoris [PDF]

Applied Microbiology and Biotechnology, 2009
Elastin-like polypeptides (ELPs) are biocompatible designer polypeptides with inverse temperature transition behavior in solution. They have a wide variety of possible applications and a potential medical importance. Currently, production of ELPs is done at lab scale in Escherichia coli shake flask cultures. With a view to future large scale production,
Schipperus, R., Teeuwen, R.L.M., Werten, M.W.T., Eggink, G., de Wolf, F.A.   +4 more
openaire   +3 more sources